The concentrations of the prostaglandins E and F, 13 14-dihydro-15-0x0-prostaglandin F and thromboxane B2 in tissues obtained from women with and without pre-eclampsia

1979 ◽  
Vol 3 (4) ◽  
pp. 223-234 ◽  
Author(s):  
J.S. Robinson ◽  
C.W.G. Redman ◽  
L. Clover ◽  
M.D. Mitchell
Keyword(s):  
Thromboxane B2 ◽  
Prostaglandin F ◽  
Prostaglandins E

PRODUCTION OF THROMBOXANE B2 BY INTRA-UTERINE TISSUES FROM PREGNANT GOATS IN VITRO

1978 ◽  
Vol 78 (1) ◽  
pp. 159-160 ◽  
Author(s):  
M. D. MITCHELL ◽  
A. P. F. FLINT ◽  
E. J. KINGSTON ◽  
G. D. THORBURN ◽  
J. S. ROBINSON
Keyword(s):  
Thromboxane B2 ◽  
Basic Data ◽  
University Of Oxford ◽  
Obstetrics And Gynaecology ◽  
Nuffield Department ◽  
John Radcliffe Hospital ◽  
Prostaglandin F ◽  
Prostaglandins E

Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington, Oxford, OX3 9DU (Received 9 February 1978) It has been shown that prostaglandins play an important role in the mechanism of parturition in many species, including the goat (Currie & Thorburn, 1977; Thorburn, Challis & Robinson, 1977). Recently we have demonstrated that intra-uterine tissues from pregnant goats, when superfused in vitro, produce prostaglandins E and F (PGE, PGF) and 13,14-dihydro-15-oxo-prostaglandin F at various rates (Mitchell, Flint, Robinson & Thorburn, 1978). The exciting discoveries of two potent prostaglandin-like compounds, thromboxane A2 (TXA2; Hamberg, Svensson & Samuelsson, 1975) and prostacyclin (PGI2; Moncada, Gryglewski, Bunting & Vane, 1976), have radically altered our thinking on prostaglandins and basic data are urgently required concerning these compounds. Since prostaglandin endoperoxides are the immediate precursors of both prostaglandins and TXA2 (and PGI2) and since TXA2 has been shown to cause contraction of a number

PROSTAGLANDIN E, THROMBOXANE B2 AND 6-OXO-PROSTAGLANDIN F1α IN AMNIOTIC FLUID AND MATERNAL PLASMA OF RHESUS MONKEYS (MACACA MULATTA) DURING THE LATTER THIRD OF GESTATION

1979 ◽  
Vol 81 (3) ◽  
pp. 345-349 ◽  
Author(s):  
J. S. ROBINSON ◽  
R. NATALE ◽  
L. CLOVER ◽  
M. D. MITCHELL
Keyword(s):  
Rhesus Monkey ◽  
Amniotic Fluid ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Maternal Plasma ◽  
Thromboxane B2 ◽  
Prostaglandin E ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
Serial Samples

The concentrations of prostaglandin E (PGE), thromboxane B2 (TXB2) and 6-oxo-prostaglandin F1α (6-oxo-PGF1α) were measured by radioimmunoassay in serial samples of amniotic fluid and maternal peripheral plasma in the latter third of pregnancy in rhesus monkeys (Macaca mulatta). The samples were collected under ketamine-induced anaesthesia. The concentration of PGE was undetectable in amniotic fluid until a few days before delivery when a large increase was observed in three of the five animals. There were small increases of TXB2 and 6-oxo-PGF1α in amniotic fluid before delivery. In maternal plasma the concentrations of PGE, TXB2 and 6-oxo-PGF1α were generally higher and more variable than in amniotic fluid and did not increase with advancing gestation. It is suggested that increased production of primary prostaglandins occurs before, and is involved in, the onset of parturition in the rhesus monkey.

Utilization of a single antiserum for the direct radioimmunoassay of prostaglandins E and F in semen and prostaglandin F in amniotic fluid

1974 ◽  
Vol 7 (5) ◽  
pp. 433-442 ◽  
Author(s):  
Alan H. Clarke ◽  
Raymond M.Y. Ing ◽  
Warren R. Jones ◽  
Derek Llewellyn-Jones ◽  
Donald A. Shutt
Keyword(s):  
Amniotic Fluid ◽  
Prostaglandin F ◽  
Prostaglandins E

CONCENTRATIONS OF 6-OXO-PROSTAGLANDIN F1α AND THROMBOXANE B2 IN THE TRACHEAL FLUID OF FOETAL SHEEP

1978 ◽  
Vol 79 (3) ◽  
pp. 403-404
Author(s):  
M. D. MITCHELL ◽  
J. S. ROBINSON
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
University Of Oxford ◽  
Obstetrics And Gynaecology ◽  
Nuffield Department ◽  
John Radcliffe Hospital ◽  
Prostaglandin F ◽  
Tracheal Insufflation ◽  
Prostaglandins E

Nuffield Department of Obstetrics and Gynaecology, University of Oxford, John Radcliffe Hospital, Headington, Oxford, OX3 9DU (Received 21 July 1978) Prostaglandins of the E and F series have been shown to relax or contract respectively guinea-pig trachea and recently it has been demonstrated that the prostaglandin endoperoxides PGG2 and PGH2 (immediate precursors of prostaglandins E and F) have far greater potency in contracting tracheal smooth muscle than prostaglandin F (Hamberg, Svensson, Hedqvist, Strandberg & Samuelsson, 1976). Furthermore, both thromboxane A2 and prostacyclin can also elicit contraction of the trachea with thromboxane A2 being considerably more active (Omini, Moncada & Vane, 1977; Svensson, Strandberg, Tuvemo & Hamberg, 1977). Administration of thromboxane A2 has been shown to raise the tracheal insufflation pressure in guinea-pigs (Svensson et al. 1977) and indeed its stable metabolite thromboxane B2 is released in relatively large amounts from sensitized guinea-pig lungs when challenged with antigen (Dawson, Boot, Cockerill,

Inhibition of the n-formylmethionyl-leucyl-phenylalanine induced respiratory burst in human neutrophils by adrenergic agonists and prostaglandins of the E series

1981 ◽  
Vol 59 (9) ◽  
pp. 915-920 ◽  
Author(s):  
Kenneth Wong ◽  
Karen Freund
Keyword(s):  
Adenylate Cyclase ◽  
Respiratory Burst ◽  
Human Neutrophils ◽  
Superoxide Anions ◽  
Adrenergic Agonists ◽  
Active Inhibition ◽  
Prostaglandin F ◽  
Membrane Bound ◽  
Prostaglandins E

Exogenous prostaglandins E1 and E2 and L-isoproterenol potently inhibited the production of superoxide anions by human neutrophils activated in vitro by n-formylmethionyl-leucyl-phenylalanine (FMLP). An estimated ID50 of 50 nM was found for all three agents while L-epinephrine and prostaglandin F2α were 10 and 100 fold, respectively, less active. Inhibition occurred whether these agents were added before, together with, or after the addition of the tripeptide to cell suspensions. Cells treated with dibutyryl adenosine 3′,5′-monophosphate also expressed reduced rates of superoxide synthesis thus suggesting that the hormonal inhibitors acted indirectly by stimulating membrane bound adenylate cyclase.

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