Characterization of resting and phorbol ester or concanavalin A activated bovine lymph node cells with leukocyte specific monoclonal antibodies

1994 ◽  
Vol 40 (1) ◽  
pp. 49-61 ◽  
Author(s):  
David J. Hurley ◽  
Richard A. Wilson ◽  
Cynthia L. Baldwin ◽  
Jing-Yi Liu ◽  
Andrea M. Mastro
Keyword(s):  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Phorbol Ester ◽  
Concanavalin A ◽  
Lymph Node Cells

scholarly journals Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wenbo Jiang ◽  
Julius Wong ◽  
Hyon-Xhi Tan ◽  
Hannah G. Kelly ◽  
Paul G. Whitney ◽  
...  
Keyword(s):  
Animal Model ◽  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Cross Reactivity ◽  
Tfh Cells ◽  
Follicular Helper ◽  
Lymph Node Cells ◽  
Human Viruses ◽  
Humoral Responses ◽  
Murine Monoclonal Antibodies

AbstractThe ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre‐clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret‐reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2.

scholarly journals Specific immunotherapy with tumour‐draining lymph node cells cultured with both anti‐CD3 and anti‐CD28 monoclonal antibodies

Immunology ◽  
1996 ◽  
Vol 87 (3) ◽  
pp. 447-453 ◽  
Author(s):  
M. HARADA ◽  
T. OKAMOTO ◽  
K. OMOTO ◽  
K. TAMADA ◽  
M. TAKENOYAMA ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Lymph Node ◽  
Specific Immunotherapy ◽  
Lymph Node Cells ◽  
Draining Lymph Node ◽  
Cells Cultured

scholarly journals T-cell-specific murine Ia antigens: serology of I-J and I-E subregion specificities.

1978 ◽  
Vol 148 (3) ◽  
pp. 692-703 ◽  
Author(s):  
C E Hayes ◽  
F H Bach
Keyword(s):  
Lymph Node ◽  
T Cell ◽  
Concanavalin A ◽  
Lymphocyte Activation ◽  
Antigenic Determinants ◽  
Spleen Cells ◽  
Cell Reactivity ◽  
Functional Studies ◽  
Lymph Node Cells ◽  
T Cell Subpopulations

(B10 X B10.D2)F1 mice were immunized with B10.A(5R) concanavalin A-stimulated thymocyte blasts. The genetic disparity between donor and recipient was restricted to the I-J and I-E subregions of the murine major histocompatibility (H-2) complex. A high-titered, T-cell-specific anti I-JkEk serum was obtained. The antiserum lysed 27-30% of haplotype k, q, or s lymph node cells, 5.3 +/- 2% of haplotype k spleen cells, and did not lyse thymocytes. Nylon wool-passed lymph node or spleen cells (H-2k) showed considerable reactivity with anti-I-JkEk serum (35-40% lysis); anti-Thy1.2 plus complement-treated spleen cells did not react (less than 5% lysis). I-Ek antibody was detected by B10.A(3R) lymph node cell reactivity (20% lysis), whereas reaction with H-2k lymph node cells after B10.A(3R) absorption demonstrated IJk antibody (12% lysis). Lymphocyte activation with alloantigen or mitogen led to increased anti-I-JkEk serum reactivity. These results, showing antibody production to at least two T-cell Ia antigenic determinants by concanavalin A thmocyte blast immunization, suggest that a group of I-region-encoded T-cell specificities may not have been detected using conventional immunization protocols because they would not have comprised a major antigenic component of the immunizing cell population. The existence of multiple Ia antigenic determinants unique to T lymphocytes would have important implications for serological and functional studies of T-cell subpopulations.

scholarly journals Characterization of Lyt-2-, L3T4- class I-specific cytolytic clones in C3H-gld/gld mice. Implications for functions of accessory molecules and programmed development.

1987 ◽  
Vol 166 (4) ◽  
pp. 1026-1040 ◽  
Author(s):  
K Yui ◽  
Y Hashimoto ◽  
S Wadsworth ◽  
M I Greene
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Gene Activation ◽  
Class I ◽  
Target Cells ◽  
Antigen Specificity ◽  
Lymph Node Cells ◽  
Accessory Molecules ◽  
Alpha Beta

We report the first demonstration of Thy-1+, Lyt-2-, L3T4- MHC-specific CTL clones derived from the Lyt-2-, L3T4- subset of lymph node cells of C3H-gld/gld mice. These clones express alpha/beta heterodimeric TCRs on the cell surface and specifically recognize class I molecules on target cells. Lyt-2 and L3T4 molecules are therefore not essential for the induction, recognition, and killing of antigen-specific CTL. In addition, these studies suggest that antigen specificity development for class I structures may occur before Lyt-2 gene activation in the differentiation of T cells.

scholarly journals The capacity of mitogen-responsive T cells to home to their tissue of origin, analyzed by means of chromosome markers

1976 ◽  
Vol 143 (3) ◽  
pp. 660-671 ◽  
Author(s):  
MJ Doenhoff ◽  
AJS Davies
Keyword(s):  
T Cells ◽  
Lymph Node ◽  
Concanavalin A ◽  
Spleen Cells ◽  
Con A ◽  
Syngeneic Mouse ◽  
Lymph Node Cells ◽  
Mitogen Responsiveness ◽  
Tissue Of Origin ◽  
Syngeneic Recipient

Lance and Taub (1) showed that when radioactively labeled lymphocytes were injected into a syngeneic mouse and the lymph node cells of this animal transferred to a second syngeneic recipient, the proportion of radioactivity found in the lymph node relative to the amount present in the spleen of the secondary recipient had increased markedly. The interpretation of this result was that some lymphocytes have the capacity to home to their organ of origin. The purpose of the experiments described here was to test the homing copacity of T cells by a method that did not involve radioactive labeling. It has been shown elsewhere that some or all mouse T cells are stimulated to divide in culture by the mitogens phytohemagglutinin (PHA) and concanavalin A (Con A) (2). We therefore elected to inject karyotypically distinct lymphocytes into syngeneic recipients and to follow their subsequent distribution by culture of lymph node and spleen cells of the recipient with PHA or Con A. In this manner the homing capacities of spleen and lymph node T cells could be determined, and furthermore, the effects of labeling with chromium-51 ((51)Cr) could be assayed with respect to the persistence of mitogen responsiveness in the injected cells.

Enumeration and Characterization of Bovine Blood, Spleen and Lymph Node Cells Containing Immunoglobulins

1980 ◽  
Vol 61 (1) ◽  
pp. 100-106 ◽  
Author(s):  
Robert Rothlein ◽  
Donald W. Johnson ◽  
Charles C. Muscoplat
Keyword(s):  
Lymph Node ◽  
Bovine Blood ◽  
Lymph Node Cells
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