ABSTRACTUpon invasion of Lewis rat macrophages,Toxoplasmarapidly induces programmed cell death (pyroptosis), which preventsToxoplasmareplication, possibly explaining the resistance of the Lewis rat toToxoplasma. Using a chemical mutagenesis screen, we identifiedToxoplasmamutants that no longer induced pyroptosis. Whole-genome sequencing led to the identification of threeToxoplasmaparasitophorous vacuole-localized dense granule proteins, GRA35, GRA42, and GRA43, that are individually required for induction of Lewis rat macrophage pyroptosis. Macrophage infection with Δgra35, Δgra42, and Δgra43parasites led to greatly reduced cell death rates and enhanced parasite replication. Lewis rat macrophages infected with parasites containing a single, double, or triple deletion of these GRAs showed similar levels of cell viability, suggesting that the three GRAs function in the same pathway. Deletion ofGRA42orGRA43resulted in GRA35 (and other GRAs) being retained inside the parasitophorous vacuole instead of being localized to the parasitophorous vacuole membrane. Despite having greatly enhanced replication in Lewis rat macrophagesin vitro, Δgra35, Δgra42, and Δgra43parasites did not establish a chronic infection in Lewis rats.Toxoplasmadid not induce F344 rat macrophage pyroptosis, but F344 rats infected with Δgra35, Δgra42, and Δgra43parasites had reduced cyst numbers. Thus, these GRAs determined parasitein vivofitness in F344 rats. Overall, our data suggest that these threeToxoplasmadense granule proteins play a critical role in establishing a chronic infectionin vivo, independently of their role in mediating macrophage pyroptosis, likely due to their importance in regulating protein localization to the parasitophorous vacuole membrane.IMPORTANCEInflammasomes are major components of the innate immune system and are responsible for detecting various microbial and environmental danger signals. Upon invasion of Lewis rat macrophages, the parasite rapidly activates the NLRP1 inflammasome, resulting in pyroptosis and elimination of the parasite’s replication niche. The work reported here revealed thatToxoplasmaGRA35, GRA42, and GRA43 are required for induction of Lewis rat macrophage pyroptosis. GRA42 and GRA43 mediate the correct localization of other GRAs, including GRA35, to the parasitophorous vacuole membrane. These three GRAs were also found to be important for parasitein vivofitness in aToxoplasma-susceptible rat strain, independently of their role in NLRP1 inflammasome activation, suggesting that they perform other important functions. Thus, this study identified three GRAs that mediate the induction of Lewis rat macrophage pyroptosis and are required for pathogenesis of the parasite.