Cell and tissue-specific expression of a heterologous gene under control of the myelin basic protein gene promoter in transgenic mice

1992 ◽  
Vol 65 (2) ◽  
pp. 217-221 ◽  
Author(s):  
Robin Miskimins ◽  
Loren Knapp ◽  
Michael J. Dewey ◽  
Xiaoping Zhang
Keyword(s):  
Transgenic Mice ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Protein Gene ◽  
Gene Promoter ◽  
Heterologous Gene ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Myelin Basic Protein Gene

Expression of the Myelin Basic Protein Gene in Transgenic Mice Expressing Human Neurotropic Virus, JCV, Early Protein

Virology ◽  
1994 ◽  
Vol 202 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Susan Haas ◽  
Nasreen S. Haque ◽  
Alan H. Beggs ◽  
Kamel Khalili ◽  
Robert L. Knobler ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Protein Gene ◽  
Neurotropic Virus ◽  
Early Protein ◽  
Myelin Basic Protein Gene

Cloning and characterization of the rat myelin basic protein gene promoter

Gene ◽  
2003 ◽  
Vol 313 ◽  
pp. 161-167 ◽  
Author(s):  
Qiou Wei ◽  
W.Keith Miskimins ◽  
Robin Miskimins
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Protein Gene ◽  
Gene Promoter ◽  
Myelin Basic Protein Gene

scholarly journals Myelin basic protein gene contains separate enhancers for oligodendrocyte and Schwann cell expression.

1992 ◽  
Vol 119 (3) ◽  
pp. 605-616 ◽  
Author(s):  
A Gow ◽  
V L Friedrich ◽  
R A Lazzarini
Keyword(s):  
Myelin Basic Protein ◽  
Schwann Cells ◽  
Basic Protein ◽  
Protein Gene ◽  
Core Promoter ◽  
Cell Types ◽  
Specific Expression ◽  
Endogenous Gene ◽  
Myelin Basic Protein Gene ◽  
Dna Fragment

The DNA sequence between position +36 and -1907 of the murine myelin basic protein gene contains the enhancer and promoter elements necessary for abundant and cell specific expression in transgenic mice. Surprisingly, the pattern of expression promoted by this DNA fragment is a subset of that exhibited by the endogenous myelin basic protein (MBP) gene. Fusion genes prepared with this promoter/enhancer and a Lac Z reporter gene are expressed only in oligodendrocytes and not in Schwann cells, whereas the endogenous MBP gene is expressed in both cell types. The level of transgene expression measured by nuclear run-on experiments is very substantial and rivals that of the endogenous MBP gene. Furthermore, this 1.9-kb DNA fragment directs transcription on the same (or very similar) developmental schedule as the endogenous gene. These results indicate that the MBP promoter/enhancer sequences are at least tripartite: a core promoter, the oligodendrocyte enhancer elements, and a third component that either expands the specificity of the oligodendrocyte enhancer to include Schwann cells or acts independently to specifically stimulate transcription in Schwann cells.

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