Cloning and characterization of the ribosomal RNA gene repeat from Ostertagia ostertagi

AbstractA molecular study was undertaken to clarify the identity of the photobiont in colourmorphs of the lichen, Peltigera membranacea. Two strains of cyanobacteria, identified as Nosroc sp. by morphology, were cultivated from each of two lichen specimens. Prokaryotic (16S) ribosomal RNA gene fragments were amplified by the polymerase chain reaction (PCR) from DNA extracted from the isolated strains and the lichens, and sequenced directly. Sequences were 98 1% identical between lichen specimens, TDI#AR94 and TDI#AR95, and highly similar to sequences published, or generated in this study from a type culture, for Nostoc. The 16S ribosomal RNA gene sequences (‘ 16S rDNA’) of all four lichen-derived cyanobacteria appeared the same, even though the lichen specimens from which they originated had different sequences. The 16S rDXA from strains 9A and 9B were different from that of specimen TDI#AR94, the thallus from which they were isolated, and instead were the same as that of strains 10A and 10B, and their source, specimen TDI#AR95. When primers selective for the strain 9A sequence were used, however, a small amount of PCR product corresponding to the 16S rDNA of strain 9A was obtained from lichen TDI#AR94. The results confirm that the photobionts of P. membranacea belong to Nostoc, and suggest that genetic differences in the photobiont may be a factor in the occurrence of colourmorphs among cyanolichens.

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Characterization of the Trypanosoma brucei 5S ribosomal RNA gene and transcript: the 5S rRNA is a spliced-leader-independent species

Gene ◽

10.1016/0378-1119(85)90165-9 ◽

1985 ◽

Vol 35 (1-2) ◽

pp. 131-141 ◽

Cited By ~ 26

Author(s):

Michael J. Lenardo ◽

David M. Dorfman ◽

Lekkala V. Reddy ◽

John E. Donelson

Keyword(s):

Trypanosoma Brucei ◽

Ribosomal Rna ◽

5S Rrna ◽

Ribosomal Rna Gene ◽

5S Ribosomal Rna ◽

Spliced Leader ◽

Independent Species

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(284) Characterization of the Genus Corylopsis

HortScience ◽

10.21273/hortsci.40.4.1051d ◽

2005 ◽

Vol 40 (4) ◽

pp. 1051D-1051

Author(s):

Mark S. Roh ◽

Eunju Cheong ◽

Ik-Young Choi ◽

Eun-Ha Yoo ◽

Jae-Young Kim ◽

...

Keyword(s):

Ribosomal Rna ◽

Rapd Markers ◽

Morphological Characteristics ◽

Single Nucleotide ◽

Ribosomal Rna Gene ◽

Snp Data ◽

Intraspecific Variations ◽

Subspecies Level

We investigated the interspecific relationships and intraspecific variations in the genus Corylopsis using RAPD and single nucleotide polymorphism (SNP) in the internal transcribed spacer1(ITS1), 5.8S ribosomal RNA gene specific in C. glabrescens Franch. & Sav. Differences in species identification between morphological characteristics and RAPD result were noticed in some accessions. All C. glabrescens, C. coreana Uyeki, and C. glabrescens f. gotoana (Makino) T. Yamanaka accessions clustered in one major group. However, they could be divided into five subgroups that are not related to the geographical origins. For example, C. coreana accessions from Korea were clustered with C. glabrescens from Japan. Although grouping based on SNP data does not agree with that by RAPD markers, it revealed the limitation in identification and classification of the genus due to high intraspecific variations in SNP. At SNP positions 464, 465, 466, 467, and 496, most accessions of C. glabrescens and C. coreana have C, G, A, A, and T, respectively. In both analyses, C. glabrescens, C. coreana, and C. glabrescens f. gotoana Japan are closely related and this suggests that nomenclature for these three species should be discussed. Although ITS1 5.8S ribosomal RNA gene can not be used for identification at a subspecies level of C. glabrescens, it proved to be useful to differentiate C. sinensisfrom C. sinensis var. calvescens. It is suggested to use C. glabrescens f. gotoana or C. glabrescens f. coreana rather than using C. coreana.

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