scholarly journals (284) Characterization of the Genus Corylopsis

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1051D-1051
Author(s):  
Mark S. Roh ◽  
Eunju Cheong ◽  
Ik-Young Choi ◽  
Eun-Ha Yoo ◽  
Jae-Young Kim ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Rapd Markers ◽  
Morphological Characteristics ◽  
Single Nucleotide ◽  
Ribosomal Rna Gene ◽  
Snp Data ◽  
Intraspecific Variations ◽  
Subspecies Level

We investigated the interspecific relationships and intraspecific variations in the genus Corylopsis using RAPD and single nucleotide polymorphism (SNP) in the internal transcribed spacer1(ITS1), 5.8S ribosomal RNA gene specific in C. glabrescens Franch. & Sav. Differences in species identification between morphological characteristics and RAPD result were noticed in some accessions. All C. glabrescens, C. coreana Uyeki, and C. glabrescens f. gotoana (Makino) T. Yamanaka accessions clustered in one major group. However, they could be divided into five subgroups that are not related to the geographical origins. For example, C. coreana accessions from Korea were clustered with C. glabrescens from Japan. Although grouping based on SNP data does not agree with that by RAPD markers, it revealed the limitation in identification and classification of the genus due to high intraspecific variations in SNP. At SNP positions 464, 465, 466, 467, and 496, most accessions of C. glabrescens and C. coreana have C, G, A, A, and T, respectively. In both analyses, C. glabrescens, C. coreana, and C. glabrescens f. gotoana Japan are closely related and this suggests that nomenclature for these three species should be discussed. Although ITS1 5.8S ribosomal RNA gene can not be used for identification at a subspecies level of C. glabrescens, it proved to be useful to differentiate C. sinensisfrom C. sinensis var. calvescens. It is suggested to use C. glabrescens f. gotoana or C. glabrescens f. coreana rather than using C. coreana.

Cloning and characterization of the ribosomal RNA gene repeat from Ostertagia ostertagi

1991 ◽  
Vol 45 (2) ◽  
pp. 275-280 ◽  
Author(s):  
John B. Dame ◽  
Charles A. Yowell ◽  
Charles H. Courtney ◽  
W.Gary Lindgren
Keyword(s):  
Ribosomal Rna ◽  
Ostertagia Ostertagi ◽  
Ribosomal Rna Gene

I-rDNA and C16S: Identification and Classification of Ribosomal RNA Gene Fragments

2013 ◽  
pp. 1-12
Author(s):  
Sharmila Mande ◽  
Tarini Shankar Ghosh ◽  
Mohammed Monzoorul Haque
Keyword(s):  
Ribosomal Rna ◽  
Ribosomal Rna Gene

scholarly journals Characterization of Five New Earthworm Mitogenomes (Oligochaeta: Lumbricidae): Mitochondrial Phylogeny of Lumbricidae

Diversity ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 580
Author(s):  
Hongyi Liu ◽  
Yufeng Zhang ◽  
Wei Xu ◽  
Yu Fang ◽  
Honghua Ruan
Keyword(s):  
Phylogenetic Trees ◽  
Mitochondrial Gene ◽  
Morphological Characteristics ◽  
Molecular Techniques ◽  
Phylogenetic Studies ◽  
Octolasion Tyrtaeum ◽  
Eisenia Nordenskioldi ◽  
Novel Strategy

Identification based on conventional morphological characteristics is typically difficult and time-consuming. The development of molecular techniques provides a novel strategy that relies on specific mitochondrial gene fragments to conduct authentication. For this study, five newly sequenced partial mitogenomes of earthworms (Bimastos parvus, Dendrobaena octaedra, Eisenia andrei, Eisenia nordenskioldi, and Octolasion tyrtaeum) with lengths ranging from 14,977 to 15,715 were presented. Each mitogenome possessed a putative control region that resided between tRNA-Arg and tRNA-His. All of the PCGs were under negative selection according to the value of Ka/Ks. The phylogenetic trees supported the classification of Eisenia and Lumbricus; however, the trees based on cox1 did not. Through various comparisons, it was determined that cox1 fragments might be more suitable for molecular identification. These results lay the foundation for further phylogenetic studies on Lumbricidae.

Cloning and Characterization of Ribosomal RNA Gene of Physarum polycephalum1

1981 ◽  
Vol 89 (6) ◽  
pp. 1693-1698 ◽  
Author(s):  
Yoshiyuki SAKAKI ◽  
Michitoshi NAKAMURA ◽  
Hisayuki NOMIYAMA ◽  
Yasuyuki TAKAGI
Keyword(s):  
Ribosomal Rna ◽  
Ribosomal Rna Gene

scholarly journals Multi-Gene Phylogeny and Taxonomy of Hydnellum (Bankeraceae, Basidiomycota) from China

2021 ◽  
Vol 7 (10) ◽  
pp. 818
Author(s):  
Yan-Hong Mu ◽  
Jia-Rui Yu ◽  
Ting Cao ◽  
Xiang-Hua Wang ◽  
Hai-Sheng Yuan
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Small Subunit ◽  
Phylogenetic Position ◽  
Ribosomal Rna Gene ◽  
Morphological Studies

The genus Hydnellum is an important group of stipitate hydnaceous fungi which can form ectomycorrhiza with many species of woody plants. In recent decades, the frequency and number of basidiocarps observed in China have been declining significantly. So far, however, we know little about the species diversity of Hydnellum in China. In this study, we conducted molecular phylogenetic analyses based on sections of multiple loci, including the large subunit of nuclear ribosomal RNA gene (nLSU), the internal transcribed spacer regions (ITS), the small subunit of nuclear ribosomal RNA gene (SSU) and the second-largest subunit of RNA polymerase II gene (RPB2), as well as morphological studies, of collected samples of Hydnellum from China. We also inferred Maximum Likelihood and Bayesian phylogenies for the order Thelephorales from the dataset of the combined nLSU and ITS. This study has revealed the phylogenetic position of Hydnellum in the order Thelephorales, and phylogenetically confirmed ten major clades in Thelephorales; Twenty-nine taxa are proposed, described or reported, including 10 new subgenera (Hydnellum subgenus Hydnellum, subg. Caesispinosum, subg. Croceum, subg. Inflatum, subg. Rhizomorphum, subg. Scabrosum, subg. Spongiosum, subg. Subindufibulatum, subg. Violaceum and subg. Zonatum), 11 new species (Hydnellum atrorubrum, H. atrospinosum, H. bomiense, H. brunneorubrum, H. fibulatum, H. granulosum, H. inflatum, H. rubidofuscum, H. squamulosum, H. sulcatum and H. yunnanense), 3 newly recorded species (H. caeruleum, H. peckii and H. spongiosipes) and 5 notable specimens (Hydnellum sp 1, H. sp 2, H. sp 3, H. sp 4 and H. sp 5). A classification system based on the morphological characteristics (especially the hyphal structure types) and molecular analyses is proposed to accommodate most species in Hydnellum. The distinguishing characters of the subgenera and the new species with their closely related taxa are discussed. A key to the species of Hydnellum from China is provided.

I-rDNA and C16S: Identification and Classification of Ribosomal RNA Gene Fragments

2015 ◽  
pp. 316-327
Author(s):  
Sharmila Mande ◽  
Tarini Shankar Ghosh ◽  
Mohammed Monzoorul Haque
Keyword(s):  
Ribosomal Rna ◽  
Ribosomal Rna Gene
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