The structural genes for nitric oxide reductase from Pseudomonas aeruginosa

1995 ◽  
Vol 1261 (2) ◽  
pp. 279-284 ◽  
Author(s):  
Hiroyuki Arai ◽  
Yasuo Igarashi ◽  
Tohru Kodama
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Nitric Oxide Reductase ◽  
Structural Genes

scholarly journals Pseudomonas aeruginosa overexpression system of nitric oxide reductase for in vivo and in vitro mutational analyses

2018 ◽  
Vol 1859 (5) ◽  
pp. 333-341 ◽  
Author(s):  
Raika Yamagiwa ◽  
Takuya Kurahashi ◽  
Mariko Takeda ◽  
Mayuho Adachi ◽  
Hiro Nakamura ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Nitric Oxide Reductase ◽  
Overexpression System

The Requirement of RpoN (Sigma Factor ς54) in Denitrification by Pseudomonas stutzeri Is Indirect and Restricted to the Reduction of Nitrite and Nitric Oxide

1998 ◽  
Vol 64 (8) ◽  
pp. 3092-3095 ◽  
Author(s):  
Elisabeth Härtig ◽  
Walter G. Zumft
Keyword(s):  
Nitric Oxide ◽  
Nitrite Reductase ◽  
Sigma Factor ◽  
Pseudomonas Stutzeri ◽  
Nitrous Oxide Reductase ◽  
Null Mutant ◽  
Nitric Oxide Reductase ◽  
Structural Genes ◽  
Wild Type ◽  
Respiratory Nitrate Reductase

ABSTRACT The rpoN region of Pseudomonas stutzeri was cloned, and an rpoN null mutant was constructed. RpoN was not essential for denitrification in this bacterium but affected the expression levels and enzymatic activities of cytochromecd 1 nitrite reductase and nitric oxide reductase, whereas those of respiratory nitrate reductase and nitrous oxide reductase were comparable to wild-type levels. Since the transcription of the structural genes nirS andnorCB, coding for nitrite reductase and the nitric oxide reductase complex, respectively, proceeded unabated, our data indicate a posttranslational process for the two key enzymes of denitrification depending on RpoN.

scholarly journals An integrated network analysis reveals that nitric oxide reductase prevents metabolic cycling of nitric oxide by Pseudomonas aeruginosa

2017 ◽  
Vol 41 ◽  
pp. 67-81 ◽  
Author(s):  
Jonathan L. Robinson ◽  
Jacob M. Jaslove ◽  
Allison M. Murawski ◽  
Christopher H. Fazen ◽  
Mark P. Brynildsen
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Network Analysis ◽  
Nitric Oxide Reductase ◽  
Integrated Network

Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages

2007 ◽  
Vol 355 (2) ◽  
pp. 587-591 ◽  
Author(s):  
Kohei Kakishima ◽  
Akiko Shiratsuchi ◽  
Azuma Taoka ◽  
Yoshinobu Nakanishi ◽  
Yoshihiro Fukumori
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Activated Macrophages ◽  
Nitric Oxide Reductase

scholarly journals 3P120 Purification and crystallization of membrane bound nitric oxide reductase from Pseudomonas aeruginosa

2005 ◽  
Vol 45 (supplement) ◽  
pp. S233
Author(s):  
T. Hino ◽  
H. Kumita ◽  
Y. Fukumori ◽  
Y. Shiro
Keyword(s):  
Nitric Oxide ◽  
Pseudomonas Aeruginosa ◽  
Nitric Oxide Reductase ◽  
Membrane Bound

scholarly journals Cephalosporin nitric oxide-donor prodrug DEA-C3D disperses biofilms formed by clinical cystic fibrosis isolates of Pseudomonas aeruginosa

2019 ◽  
Vol 75 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Odel Soren ◽  
Ardeshir Rineh ◽  
Diogo G Silva ◽  
Yuming Cai ◽  
Robert P Howlin ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Clinical Isolates ◽  
Nitric Oxide Donor ◽  
Confocal Laser ◽  
Broth Microdilution Method

Abstract Objectives The cephalosporin nitric oxide (NO)-donor prodrug DEA-C3D (‘DiEthylAmin-Cephalosporin-3′-Diazeniumdiolate’) has been shown to initiate the dispersal of biofilms formed by the Pseudomonas aeruginosa laboratory strain PAO1. In this study, we investigated whether DEA-C3D disperses biofilms formed by clinical cystic fibrosis (CF) isolates of P. aeruginosa and its effect in combination with two antipseudomonal antibiotics, tobramycin and colistin, in vitro. Methods β-Lactamase-triggered release of NO from DEA-C3D was confirmed using a gas-phase chemiluminescence detector. MICs for P. aeruginosa clinical isolates were determined using the broth microdilution method. A crystal violet staining technique and confocal laser scanning microscopy were used to evaluate the effects of DEA-C3D on P. aeruginosa biofilms alone and in combination with tobramycin and colistin. Results DEA-C3D was confirmed to selectively release NO in response to contact with bacterial β-lactamase. Despite lacking direct, cephalosporin/β-lactam-based antibacterial activity, DEA-C3D was able to disperse biofilms formed by three P. aeruginosa clinical isolates. Confocal microscopy revealed that DEA-C3D in combination with tobramycin produces similar reductions in biofilm to DEA-C3D alone, whereas the combination with colistin causes near complete eradication of P. aeruginosa biofilms in vitro. Conclusions DEA-C3D is effective in dispersing biofilms formed by multiple clinical isolates of P. aeruginosa and could hold promise as a new adjunctive therapy to patients with CF.

scholarly journals Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin

FEBS Journal ◽  
2006 ◽  
Vol 274 (3) ◽  
pp. 677-686 ◽  
Author(s):  
João B. Vicente ◽  
Francesca M. Scandurra ◽  
João V. Rodrigues ◽  
Maurizio Brunori ◽  
Paolo Sarti ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Escherichia Coli ◽  
Electron Transfer ◽  
Nitric Oxide Reductase ◽  
Kinetics Of
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