Properties and purification of the catalytic subunit of cyclic AMP-dependent protein kinase of adipose tissue

1982 ◽  
Vol 721 (4) ◽  
pp. 434-440 ◽  
Author(s):  
Peter Strålfors ◽  
Per Belfrage
Keyword(s):  
Adipose Tissue ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Dependent Protein

scholarly journals Studies of rat adipose-tissue microsomal glycerol phosphate acyltransferase

1984 ◽  
Vol 224 (1) ◽  
pp. 101-108 ◽  
Author(s):  
G A Nimmo ◽  
H G Nimmo
Keyword(s):  
Adipose Tissue ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Microsomal Protein ◽  
Catalytic Subunit ◽  
Glycerol Phosphate ◽  
Dependent Protein Kinase ◽  
Phosphorylated Proteins ◽  
Dependent Protein ◽  
Rat Adipose Tissue

Incubation of rat adipose-tissue microsomal fractions with iodoacetate caused an inactivation of glycerol phosphate acyltransferase that could be prevented by the presence of palmitoyl-CoA. A microsomal protein of subunit Mr 54 000 was found to react with radioactively labelled iodoacetate in the absence, but not in the presence, of palmitoyl-CoA. It is suggested that this protein is a component of glycerol phosphate acyltransferase. Incubation of rat adipose-tissue microsomal fractions with the catalytic subunit of cyclic AMP-dependent protein kinase, ATP and Mg2+ caused an inactivation of glycerol phosphate acyltransferase whose magnitude depended on the conditions used for assay of the acyltransferase. Rat adipose tissue microsomal proteins were phosphorylated by using protein kinase and [gamma-32P]ATP. One of the phosphorylated proteins was very similar, but not identical, in mobility to the Mr-54 000 protein labelled by iodoacetate. In contrast with a previous report [Sooranna & Saggerson (1976) FEBS Lett. 64, 36-39], no changes could be detected in the activity of glycerol phosphate acyltransferase in adipocytes treated with adrenaline. Adipocytes were labelled with [32P]Pi and treated with adrenaline, but no 32P was incorporated into the Mr-54000 protein labelled by iodoacetate. The results suggest that the activity of adipose-tissue microsomal glycerol phosphate acyltransferase is not directly controlled by phosphorylation.

scholarly journals A relative of the catalytic subunit of cyclic AMP-dependent protein kinase in Aplysia spermatozoa.

1990 ◽  
Vol 10 (12) ◽  
pp. 6775-6780 ◽  
Author(s):  
S Beushausen ◽  
H Bayley
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Cyclic Nucleotide ◽  
Aplysia Californica ◽  
Catalytic Subunit ◽  
Spermatogenic Cells ◽  
Dependent Protein Kinase ◽  
Dependent Protein ◽  
Mature Spermatozoa ◽  
Kinase Subfamily

Transcripts encoding CAPL-B, an apparent member of the cyclic-nucleotide-regulated kinase subfamily in Aplysia californica, are found exclusively in the ovotestis and are concentrated in meiotic and postmeiotic spermatogenic cells. The CAPL-B polypeptide is present in mature spermatozoa, suggesting that the kinase plays a part in regulating events associated with fertilization.

scholarly journals The inhibitor protein of the cyclic AMP-dependent protein kinase-catalytic subunit interaction. Composition of multiple complexes

1988 ◽  
Vol 256 (3) ◽  
pp. 785-789 ◽  
Author(s):  
S M Van Patten ◽  
A Hotz ◽  
V Kinzel ◽  
D A Walsh
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Catalytic Subunit ◽  
Inhibitor Protein ◽  
Multiple Forms ◽  
Subunit Interaction ◽  
Dependent Protein Kinase ◽  
Bovine Heart ◽  
Dependent Protein ◽  
Multiple Species

It has been previously demonstrated that the combination of pure preparations of the inhibitor protein of the cyclic AMP-dependent protein kinase and the catalytic subunit of this enzyme resulted in the formation of multiple complexes [Van Patten, Fletcher & Walsh (1986) J. Biol. Chem. 261, 5514-5523]. In the present study it is demonstrated that these multiple species occur because the bovine heart protein kinase preparation contains multiple forms of catalytic subunit [Kinzel, Hotz, König, Gagelmann, Pyerin, Reed, Köbler, Hofmann, Obst, Gensheimer, Goldblatt & Shaltiel (1987) Arch. Biochem. Biophys. 253, 341-349].

Catalytic Subunit of Cyclic AMP-Dependent Protein Kinase

1999 ◽  
Vol 82 (2-3) ◽  
pp. 133-141 ◽  
Author(s):  
Susan S Taylor ◽  
Elzbieta Radzio-Andzelm ◽  
Madhusudan ◽  
Xiaodong Cheng ◽  
Lynn Ten Eyck ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cyclic Amp ◽  
Catalytic Subunit ◽  
Dependent Protein Kinase ◽  
Dependent Protein
Sign in / Sign up

Export Citation Format

Share Document