Effects of insulin-like growth factor-I and transforming growth factor-β on MG-63 human osteosarcoma cells

1992 ◽  
Vol 17 ◽  
pp. 201
Author(s):  
A. Pirskanen ◽  
P.H. Mäenpää
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Insulin Like Growth Factor ◽  
Osteosarcoma Cells ◽  
Factor I ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Growth Factor I

scholarly journals Insulin-like growth factor I activates insulin receptor substrate 1 and Ras in human osteosarcoma cells.

1999 ◽  
Vol 46 (1) ◽  
pp. 117-123 ◽  
Author(s):  
W Lopaczynski ◽  
C Terry
Keyword(s):  
Growth Factor ◽  
Cell Line ◽  
Osteosarcoma Cell ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Human Osteosarcoma ◽  
Nuclear Extracts ◽  
Human Osteosarcoma Cells ◽  
Igf I ◽  
Growth Factor I

Insulin-like growth factor I (IGF-I) stimulates multiplication of the human osteosarcoma cell line, MG-63, by acting through IGF-I receptor. We have characterized IGF-I stimulated phosphorylation of IRS-1, activation of Ras cycle and phosphorylation of c-Jun in this cell line. Serum starved MG-63 cells were (1) IGF-I stimulated and lysates were immunoprecipitated with polyclonal IRS-1 antibody or (2) metabolically labeled with [32P]orthophosphoric acid and then cells were treated with IGF-I. Cell lysates were immunoprecipitated with p21Ras antibody (Y13-259) and bound nucleotides were analysed by thin-layer chromatography. We demonstrated tyrosine phosphorylation of IRS-1/2 immunoprecipitated from MG-63 cells stimulated with IGF-I. We also showed an increased level of GTP in p21Ras immunoprecipitates from IGF-I treated cells. Nuclear extracts prepared from 32P-labeled cells before and after addition of IGF-I were immunoprecipitated with c-Jun antibody. After electrophoresis and autoradiography, phosphorylation of the c-Jun band was seen to be IGF-I independent. Phosphoamino acid analysis of the c-Jun band showed that phosphoserine was the major species.

scholarly journals Expression of insulin-like growth factor-I and transforming growth factor-β in hypokalemic nephropathy in the rat

2001 ◽  
Vol 59 (1) ◽  
pp. 96-105 ◽  
Author(s):  
Tanny Tsao ◽  
Janet Fawcett ◽  
Fernando C. Fervenza ◽  
Fay W. Hsu ◽  
Phillip Huie ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I
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