Enhancement of antitumor activity of branchd β-1,3-glucans by chemical modification

The avarone/avarol quinone/hydroquinone couple shows considerable antitumor activity. In this work, covalent modification of ?-lactoglobulin by avarone and its derivatives as well as by the synthetic steroidal quinone 2,5(10)-estradiene- 1,4,17-trione and its derivatives were studied. The techniques for studying chemical modification of ?-lactoglobulin by quinones were: UV/Vis spectrophotometry, SDS PAGE and isoelectrofocusing. SDS PAGE results suggest that polymerization of the protein occurs. It could be seen that the protein of 18 kD gives the bands of 20 kD, 36 kD, 40 kD, 45 kD, 64 kD and 128 kD depending on modification agent. The shift of the pI of the protein (5.4) upon modification toward lower values (from pI 5.0 to 5.3) indicated that lysine amino groups are the principal site of the reaction of ?-lactoglobulin with the quinones.

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Chemical modification and antitumor activity of a d-manno-d-glucan from Microellobosporia grisea

Carbohydrate Research ◽

10.1016/0008-6215(83)88148-8 ◽

1983 ◽

Vol 115 ◽

pp. 199-208 ◽

Cited By ~ 18

Author(s):

Kazuhiro Inoue ◽

Keiji Kawamoto ◽

Hiroto Nakajima ◽

Morihiro Kohno ◽

Shizuo Kadoya ◽

...

Keyword(s):

Antitumor Activity ◽

Chemical Modification

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Antitumor Activity and Chemical Modification of Polysaccharides fromNiohshimejiMushroom,Tricholma giganteum

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.60.30 ◽

1996 ◽

Vol 60 (1) ◽

pp. 30-33 ◽

Cited By ~ 39

Author(s):

Takashi Mizuno ◽

Poohong Ykohlui ◽

Tetsuya Kinoshita ◽

Cun Zhuang ◽

Hitoshi Ito ◽

...

Keyword(s):

Antitumor Activity ◽

Chemical Modification

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Chemical modification of β-lactoglobulin by quinines

Journal of the Serbian Chemical Society ◽

10.2298/jsc0305243n ◽

2003 ◽

Vol 68 (4-5) ◽

pp. 243-248 ◽

Cited By ~ 7

Author(s):

Irena Novakovic ◽

Zoran Vujcic ◽

Tatjana Bozic ◽

Natasa Bozic ◽

Nenad Milosavic ◽

...

Keyword(s):

Antitumor Activity ◽

Chemical Modification ◽

Model Compound ◽

Cow Milk ◽

Amino Groups ◽

Tert Butyl ◽

Sds Page ◽

Covalent Modifications ◽

Β Lactoglobulin

The avarone/avarol quinone/hydroquinone couple, as well as their derivatives show considerable antitumor activity. In this work, covalent modifications of ?-lactoglobulin, isolated from cow milk, by avarone, its model compound 2-tert-butyl-1,4-benzoquinone, and several of their alkylthio derivatives were studied. The techniques applied for assaying the modifications were UV/VIS spectrophotometry, SDS PAGE and isoelectrofocusing. The results of the SDS PAGE suggest that polymerisation of the protein occurs. The shift of the pI of the protein upon modification toward lower values indicates that lysine amino groups are the principal site of the reaction of ?-lactoglobulin with the quinines.

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