Inhibitory effect of anaesthesia with 2-phenoxyethanol as compared to MS222 on glucose release in isolated hepatocytes from rainbow trout (Salmo gairdneri)

LAN analyses appear to have diagnostic value in fish pathobiology and studies were undertaken to determine optima for substrate concentration, pH, reaction time, temperature, and buffer ions. Citrate ion did not inhibit LAN at anticoagulant levels, but cyanide, pyrophosphate, and EDTA had an inhibitory effect. Storage of samples at —10 and 1 C resulted in small but significant reductions of LAN activity, while at room temperature enzyme activity was rapidly lost. LAN activity was distributed among liver fractions as follows: microsomes, 12%; mitochondria, 9%; cellular sap, 37%; other, 50%. Three isozymes of LAN were found. Blood plasma contained significant amounts of LAN activity which was significantly higher in cold- than in warm-acclimated fish. However, these LAN levels were comparable when their activity was extrapolated to body temperatures.

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Temperature dependence of lipogenesis in isolated hepatocytes from rainbow trout (Salmo gairdneri)

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(86)90325-1 ◽

1986 ◽

Vol 83 (1) ◽

pp. 13-22 ◽

Cited By ~ 1

Author(s):

B. Voss ◽

H.D. Jankowsky ◽

P. Weddigen

Keyword(s):

Rainbow Trout ◽

Temperature Dependence ◽

Isolated Hepatocytes ◽

Salmo Gairdneri

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Aflatoxin B1 metabolism and DNA binding in isolated hepatocytes from rainbow trout (Salmo gairdneri)

Carcinogenesis ◽

10.1093/carcin/3.5.511 ◽

1982 ◽

Vol 3 (5) ◽

pp. 511-518 ◽

Cited By ~ 33

Author(s):

George S. Bailey ◽

Matthew J. Taylor ◽

Daniel P. Selivonchick

Keyword(s):

Rainbow Trout ◽

Dna Binding ◽

Aflatoxin B1 ◽

Isolated Hepatocytes ◽

Salmo Gairdneri

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The effect of high-protein and high-carbohydrate diets on [125I]iodoinsulin binding in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri)

British Journal Of Nutrition ◽

10.1079/bjn19830080 ◽

1983 ◽

Vol 50 (1) ◽

pp. 129-139 ◽

Cited By ~ 31

Author(s):

Richard F. Ablett ◽

Matthew J. Taylor ◽

Daniel P. Selivonchick

Keyword(s):

Skeletal Muscle ◽

Rainbow Trout ◽

Plasma Membranes ◽

Dissociation Constants ◽

Specific Binding ◽

Isolated Hepatocytes ◽

High Protein ◽

Salmo Gairdneri ◽

Binding Studies ◽

High Carbohydrate

1. [125I]iodoinsulin-binding studies in the presence of a concentration range of bovine insulin were conducted to establish specific insulin-binding levels in skeletal muscle plasma membranes and isolated hepatocytes of rainbow trout (Salmo gairdneri) reared on control, high-protein or high-carbohydrate diets.2. Negative co-operativity was observed and receptor concentrations and apparent dissociation constants established for each preparation.3. No differences of specific binding attributed to diet were detected in skeletal muscle plasma membrane preparations; however, the receptor concentration of isolated hepatocytes from high-carbohydrate-reared trout was increased. This contrasted to comparable mammalian studies.

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