Absence of a nuclear androgen receptor in isolated germinal cells of rat testis

1977 ◽  
Vol 9 (2) ◽  
pp. 159-167 ◽  
Author(s):  
J.A. Grootegoed ◽  
M.J. Peters ◽  
E. Mulder ◽  
F.F.G. Rommerts ◽  
H.J. Van Der Molen
Keyword(s):  
Androgen Receptor ◽  
Rat Testis ◽  
Germinal Cells

Temperature Effect on the Permeability of Plasma Membranes of Advanced Germinal Cells of the Rat Testis

1974 ◽  
Vol 52 (7) ◽  
pp. 586-593 ◽  
Author(s):  
Leo P. K. Lee
Keyword(s):  
Temperature Effect ◽  
Incubation Medium ◽  
Plasma Membranes ◽  
Rat Testis ◽  
Cellular Degeneration ◽  
Adult Rat ◽  
Germinal Cells

Advanced germinal cells (spermatocytes and spermatids) prepared from adult rat testes, but not other cells, released their cellular contents into the incubation medium when they were incubated at 37° at a physiological pH. Evidence was presented that the release of cellular contents was due to changes in the permeability of the plasma membranes of these cells. The hypothesis is advanced that the changes in the permeability of the plasma membranes of the advanced testicular germinal cells of the rat may be intimately associated with the cellular degeneration and cessation of spermatogenesis which occur in cryptorchid testes.

CONVERSION OF PROGESTERONE TO ANDROGENS BY NON-FLAGELLATE GERMINAL CELLS ISOLATED FROM SEMINIFEROUS TUBULES OF RAT TESTIS

1974 ◽  
Vol 60 (2) ◽  
pp. 269-NP ◽  
Author(s):  
H. J. GALENA ◽  
C. TERNER
Keyword(s):  
Significant Contribution ◽  
Isotonic Saline ◽  
Interstitial Cells ◽  
Seminiferous Tubules ◽  
Distilled Water ◽  
Rat Testis ◽  
Germinal Cells

SUMMARY A method is described for the isolation of non-flagellate germinal cells of the testis. The interstitial cells were removed by submersion of teased seminiferous tubules in distilled water. The interstitial cells exposed to water burst while the germinal cells inside the tubules remained intact. The tubules were then homogenized in isotonic saline and the non-flagellate germinal cells (spermatocytes and young spermatids) were isolated by centrifugation and filtration through a layer of Sephadex G-25 gel. On incubation with progesterone these cells produced 17α-hydroxyprogesterone, androstenedione, and testosterone. The rate of conversion of progesterone to testosterone in vitro was 0·20 μg/h/109 germinal cells. These results suggest that the non-flagellate germinal cells of the testis may make a significant contribution to the production of androgens.

scholarly journals The Effect of Cryptorchidism on the Quantitative Histology, Histochemistry and Hydrolytic Enzyme Activity of the Rat Testis

1978 ◽  
Vol 31 (1) ◽  
pp. 53 ◽  
Author(s):  
AW Blackshaw ◽  
PF Massey
Keyword(s):  
Enzyme Activity ◽  
Meiotic Prophase ◽  
Hydrolytic Enzyme ◽  
Seminiferous Tubules ◽  
Quantitative Histology ◽  
Rat Testis ◽  
Germinal Cells ◽  
Enzyme Patterns

Cryptorchidism of the mature rat testis led to degeneration of the seminiferous tubules and changes in enzyme patterns and activities. Spermatogenic stages 1--4, containing pachytene primary spermatocytes in late meiotic prophase, and stage 5, containing recently formed round spermatids, were damaged by 48 h. Within 96 h stages showed a loss of germinal cells into the lumen and this was almost complete by 192 h.

scholarly journals Regulation of androgen receptor mRNA and protein in the rat testis by testosterone

1991 ◽  
Vol 40 (1-3) ◽  
pp. 343-347 ◽  
Author(s):  
Leen J. Blok ◽  
John M.S. Bartlett ◽  
Joan Bolt-de Vries ◽  
Axel P.N. Themmen ◽  
Albert O. Brinkmann ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Rat Testis ◽  
Receptor Mrna

Androgen receptor in nuclei of rat testis

Steroids ◽  
1975 ◽  
Vol 25 (5) ◽  
pp. 569-586 ◽  
Author(s):  
Albert A. Smith ◽  
William S. McLean ◽  
Vidar Hansson ◽  
Shihadeh N. Nayfeh ◽  
Frank S. French
Keyword(s):  
Androgen Receptor ◽  
Rat Testis

scholarly journals GLYCOGEN IN NON-GERMINAL CELLS OF RAT TESTIS FOLLOWING GAMMA-IRRADIATION

Reproduction ◽  
1974 ◽  
Vol 41 (1) ◽  
pp. 185-188 ◽  
Author(s):  
G. S. GUPTA ◽  
S. R. BAWA
Keyword(s):  
Gamma Irradiation ◽  
Rat Testis ◽  
Germinal Cells

Studies on the Structure and Formation during Spermatogenesis of the Sulfoglycerogalactolipid of Rat Testis

1974 ◽  
Vol 52 (8) ◽  
pp. 689-697 ◽  
Author(s):  
M. J. Kornblatt ◽  
A. Knapp ◽  
M. Levine ◽  
H. Schachter ◽  
R. K. Murray
Keyword(s):  
Structural Studies ◽  
Peak Activity ◽  
Spermatogenic Cells ◽  
Rat Testis ◽  
Alkyl Groups ◽  
Immature Rats ◽  
Hypophysectomized Rats ◽  
Germinal Cells

Structural studies on the major glycolipid of rat testis have confirmed our earlier identification of this compound as a 1-O-alkyl-2-O-acyl-glyceryl-monogalactoside sulfate. The acyl and alkyl groups are predominantly palmitoyl and palmityl groups, respectively. The minor glycolipid found in the same tissue has been identified as the non-sulfated form of this glycolipid. Examination of the levels of the sulfoglycerogalactolipid in the testes of immature rats, hypophysectomized rats, and normal and sterile mice indicates that the majority of the compound is located in the germinal cells of the testis and that the primary spermatocytes are the earliest spermatogenic cells to contain high levels of the lipid. Galactolipid sulfotransferase also shows peak activity in the primary spermatocytes of rat testis.

Further characterization of the androgen receptor in rat testis

1976 ◽  
Vol 4 (4) ◽  
pp. 239-255 ◽  
Author(s):  
William S. McLean ◽  
Albert A. Smith ◽  
Vidar Hansson ◽  
Oddvar Naess ◽  
Shihadeh N. Nayfeh ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Rat Testis
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