Effect of several biogenic amines on in vitro progesterone secretion by the bovine corpus luteum

Abstract. While the regulation of progesterone secretion from the corpus luteum by LH has been convincingly demonstrated, the secretory patterns in the absence of any pituitary LH inputs are yet unclear. Consequently, we investigated the progesterone secretion by an in vitro perifusion system to characterize spontaneous progesterone release from the isolated bovine corpus luteum. Slices (120 mg) of midluteal corpora lutea were placed in perifusion chambers and continuously perifused by Medium-199 for 160-320 min. Progesterone was determined by radioimmunoassay in the effluent fractions collected at 2-min intervals. The spontaneous progesterone release from all bovine corpora lutea was pulsatile. Pulses were observed at mean (±sem) intervals of 17.7±1.5 min with amplitudes of 6.7±0.5 ng and release rates of 29.5±2.4 ng · ml−1 · (2 min)−1 (N=5). Addition of 6.7 nmol/l hCG to the perifusion medium appeared to increase the pulse amplitudes and release rates (195±25% over unstimulated conditions), but did not change the pulse frequencies (N=3). Perifusions with calcium-free medium containing 50 μmol/l verapamil and 20 mmol/l EGTA tended to suppress the pulse frequencies and amplitudes of this spontaneous progesterone release, whereas addition of hCG reversed this decrease again (N=3). When prostaglandin activity was inhibited by 100 μmol/l indomethacin added to the perifusion medium, both pulse frequencies and amplitudes of this progesterone release were enhanced (N=4). During perifusions with 100 μmol/l of the anti-progesterone ZK 96.734, both the pulse frequencies and amplitudes increased (N=4). These studies demonstrate an episodic progesterone release from the bovine corpus luteum perifused in vitro. The spontaneous progesterone secretion is independent of, but facilitated by gonadotropin secretion. This pulsatile progesterone secretion from the midluteal corpus luteum and its regulation by intraovarian factors may reflect an autonomous pulse generating mechanism.

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Mechanism of action of noradrenaline on secretion of progesterone and oxytocin by the bovine corpus luteum in vitro

Acta Veterinaria Hungarica ◽

10.1556/004.49.2001.1.6 ◽

2001 ◽

Vol 49 (1) ◽

pp. 39-51 ◽

Cited By ~ 12

Author(s):

Grażyna Miszkiel ◽

J. Kotwica

Keyword(s):

Corpus Luteum ◽

Mechanism Of Action ◽

Hydroxysteroid Dehydrogenase ◽

Luteal Cells ◽

Progesterone Secretion ◽

Bovine Corpus Luteum ◽

Progesterone Synthesis ◽

Luteal Tissue ◽

Prostaglandin F

The present studies were conducted: (1) to determine which β-adrenoceptor subtypes are involved in progesterone and oxytocin (OT) secretion, (2) to examine whether noradrenaline (NA) acts directly on the cytochrome P-450scc and 3β-hydroxysteroid dehydrogenase (3β-HSD), and (3) to study the effect of prostaglandin F2α, (PGF2α) on NA-stimulated steroidogenesis in luteal cells. The effect of NA on progesterone secretion from luteal slices of heifers on days 8–12 of the oestrous cycle was blocked by both atenolol (β1-antagonist) and ICI 118.551 hydrochloride (β2-antagonist). OT secretion was blocked only after treatment with ICI 118.551 hydrochloride (P < 0.05). Dobutamine (10−4−10−6), a selective β1 agonist and salbutamol (10−4−10−6), a selective β2 agonist, both increased progesterone production (P < 0.01) with an efficiency comparable to that produced by NA (P < 0.01). The increase of OT content in luteal slices was observed only after treatment with salbutamol at the dose of 10−5M (P < 0.01). Dobutamine had no effect on OT production at any dose. A stimulatory effect of NA on cytochrome P-450scc activity (P < 0.05) was demonstrated using 25-hydroxycholesterol as substrate. 3β-HSD activity also increased following NA (P < 0.01) or pregnenolone (P < 0.05) and in tissue treated with pregnenolone together with NA (P < 0.01). PGF decreased progesterone synthesis (P < 0.05) and 3β-HSD activity (P < 0.01) in tissue treated with NA. We conclude that NA stimulates progesterone secretion by luteal β1- and β2-adrenoceptors, while OT secretion is probably mediated only via the β2-receptor. NA also increases cytochrome P-450scc and 3β-HSD activity. PGF inhibits the luteotropic effect of NA on the luteal tissue.

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Biosynthesis of Progesterone, Sterols, and Squalene from Acetate-1-14C and Mevalonate-2-14C by the Bovine Corpus Luteum in Vitro

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)97410-7 ◽

1965 ◽

Vol 240 (5) ◽

pp. 1957-1961

Author(s):

Hermione R. Hellig ◽

Kenneth Savard

Keyword(s):

Corpus Luteum ◽

Bovine Corpus Luteum

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In Vitro Production of Angiotropic Factor by Bovine Corpus Luteum: Partial Characterization of Activities that are Chemotactic and Mitogenic for Endometrial Cells

Advances in Experimental Medicine and Biology - Regulation of Ovarian and Testicular Function ◽

10.1007/978-1-4684-5395-9_42 ◽

1987 ◽

pp. 683-688 ◽

Cited By ~ 8

Author(s):

D. A. Redmer ◽

J. D. Kirsch ◽

A. T. Grazul

Keyword(s):

Corpus Luteum ◽

Partial Characterization ◽

In Vitro Production ◽

Endometrial Cells ◽

Bovine Corpus Luteum ◽

Vitro Production

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Control of Steroidogenesis in Small and Large Bovine Luteal. Cells

Australian Journal of Biological Sciences ◽

10.1071/bi9870331 ◽

1987 ◽

Vol 40 (3) ◽

pp. 331 ◽

Cited By ~ 45

Author(s):

William Hansel ◽

Hector W Alila ◽

Joseph P Dowd ◽

Xiangzhong Yang

Keyword(s):

Protein Kinase C ◽

Protein Kinase ◽

Corpus Luteum ◽

Luteal Cell ◽

Lipoxygenase Pathway ◽

Luteal Cells ◽

Kinase C ◽

Bovine Corpus Luteum ◽

Progesterone Synthesis

Evidence was cited to show that: (1) prostacyclin (PGI2) plays a luteotrophic role in the bovine corpus luteum and that products of the lipoxygenase pathway of arachidonic acid metabolism, especially 5-hydroxyeicosatetraenoic acid play luteolytic roles; (2) oxytocin of luteal cell origin plays a role in development, and possibly in regression, of the bovine corpus luteum; and (3) luteal cells arise from two sources; the characteristic small luteal cells at all stages of the o~strous cycle and pregnancy are of theca cell origin; the large cells are of granulosa cell origin early in the cycle, but a population of theca-derived large cells appears later in the cycle. Results of in vitro studies with total dispersed cells and essentially pure preparations of large and small luteal cells indicate that : (1) the recently described Ca2+ -polyphosphoinositol-protein kinase C second messenger system is involved in progesterone synthesis in the bovine corpus luteum; (2) activation of protein kinase C is stimulatory to progesterone synthesis in the small luteal cells; (3) activation of protein kinase C has no effect on progesterone synthesis in the large luteal cells; and (4) protein kinase C exerts its luteotrophic effect in total cell preparations, in part at least, by stimulating the production of prostacyclin. The protein kinase C system may cause down regulation of LH receptors in the large cells.

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Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

Reproduction ◽

10.1530/rep-15-0085 ◽

2015 ◽

Vol 150 (3) ◽

pp. 217-225 ◽

Cited By ~ 35

Author(s):

Koumei Shirasuna ◽

Haruka Matsumoto ◽

Shuichi Matsuyama ◽

Koji Kimura ◽

Heinrich Bollwein ◽

...

Keyword(s):

Corpus Luteum ◽

Neutrophil Migration ◽

Peripheral Circulation ◽

Interferon Tau ◽

Bovine Corpus Luteum ◽

Activated Neutrophils ◽

Endocrine Factor ◽

Uterine Environment ◽

And Function

When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF2α. Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P4) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P4 concentrations during the maternal recognition period in cows.

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