scholarly journals Mechanism of action of noradrenaline on secretion of progesterone and oxytocin by the bovine corpus luteum in vitro

2001 ◽  
Vol 49 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Grażyna Miszkiel ◽  
J. Kotwica
Keyword(s):  
Corpus Luteum ◽  
Mechanism Of Action ◽  
Hydroxysteroid Dehydrogenase ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis ◽  
Luteal Tissue ◽  
Prostaglandin F

The present studies were conducted: (1) to determine which β-adrenoceptor subtypes are involved in progesterone and oxytocin (OT) secretion, (2) to examine whether noradrenaline (NA) acts directly on the cytochrome P-450scc and 3β-hydroxysteroid dehydrogenase (3β-HSD), and (3) to study the effect of prostaglandin F2α, (PGF2α) on NA-stimulated steroidogenesis in luteal cells. The effect of NA on progesterone secretion from luteal slices of heifers on days 8–12 of the oestrous cycle was blocked by both atenolol (β1-antagonist) and ICI 118.551 hydrochloride (β2-antagonist). OT secretion was blocked only after treatment with ICI 118.551 hydrochloride (P < 0.05). Dobutamine (10−4−10−6), a selective β1 agonist and salbutamol (10−4−10−6), a selective β2 agonist, both increased progesterone production (P < 0.01) with an efficiency comparable to that produced by NA (P < 0.01). The increase of OT content in luteal slices was observed only after treatment with salbutamol at the dose of 10−5M (P < 0.01). Dobutamine had no effect on OT production at any dose. A stimulatory effect of NA on cytochrome P-450scc activity (P < 0.05) was demonstrated using 25-hydroxycholesterol as substrate. 3β-HSD activity also increased following NA (P < 0.01) or pregnenolone (P < 0.05) and in tissue treated with pregnenolone together with NA (P < 0.01). PGF decreased progesterone synthesis (P < 0.05) and 3β-HSD activity (P < 0.01) in tissue treated with NA. We conclude that NA stimulates progesterone secretion by luteal β1- and β2-adrenoceptors, while OT secretion is probably mediated only via the β2-receptor. NA also increases cytochrome P-450scc and 3β-HSD activity. PGF inhibits the luteotropic effect of NA on the luteal tissue.

scholarly journals Control of Steroidogenesis in Small and Large Bovine Luteal. Cells

1987 ◽  
Vol 40 (3) ◽  
pp. 331 ◽  
Author(s):  
William Hansel ◽  
Hector W Alila ◽  
Joseph P Dowd ◽  
Xiangzhong Yang
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Lipoxygenase Pathway ◽  
Luteal Cells ◽  
Kinase C ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis

Evidence was cited to show that: (1) prostacyclin (PGI2) plays a luteotrophic role in the bovine corpus luteum and that products of the lipoxygenase pathway of arachidonic acid metabolism, especially 5-hydroxyeicosatetraenoic acid play luteolytic roles; (2) oxytocin of luteal cell origin plays a role in development, and possibly in regression, of the bovine corpus luteum; and (3) luteal cells arise from two sources; the characteristic small luteal cells at all stages of the o~strous cycle and pregnancy are of theca cell origin; the large cells are of granulosa cell origin early in the cycle, but a population of theca-derived large cells appears later in the cycle. Results of in vitro studies with total dispersed cells and essentially pure preparations of large and small luteal cells indicate that : (1) the recently described Ca2+ -polyphosphoinositol-protein kinase C second messenger system is involved in progesterone synthesis in the bovine corpus luteum; (2) activation of protein kinase C is stimulatory to progesterone synthesis in the small luteal cells; (3) activation of protein kinase C has no effect on progesterone synthesis in the large luteal cells; and (4) protein kinase C exerts its luteotrophic effect in total cell preparations, in part at least, by stimulating the production of prostacyclin. The protein kinase C system may cause down regulation of LH receptors in the large cells.

Involvement of high-density lipoprotein in stimulatory effect of hormones supporting function of the bovine corpus luteum.

2003 ◽  
Vol 51 (1) ◽  
pp. 111-120 ◽  
Author(s):  
D. Skarżyński ◽  
J. Młynarczuk ◽  
J. Kotwica
Keyword(s):  
Luteinising Hormone ◽  
Density Lipoprotein ◽  
High Density ◽  
High Density Lipoproteins ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis ◽  
Deficient Medium ◽  
Intracellular Pathway

The hypothesis that epinephrine (noradrenaline, NA) enhances utilisation of high-density lipoproteins (HDL) by bovine luteal cells and that this process involves phospholipase (PL) C and protein kinase (PK) C intracellular pathway was tested. Luteal cells from days 2-4, 5-10 or 11-17 of the oestrous cycle were pre-incubated for 20h. Subsequently DMEM/Ham's F-12 medium was replaced by fresh medium and the cells were treated for 6 h as follows: In Experiment I with HDL (5-75μg cholesterol per ml), NA, isoprenaline (ISO) or luteinising hormone (LH). In Experiment II cells were incubated for further 24h in deficient medium (without FCS) and next treated as in Experiment I. In Experiment III cells were stimulated with NA, ISO or LH alone and together with HDL. In Experiment IV cells were treated with PLC inhibitor (U-73122) or with PKC inhibitor (staurosporine) or stimulator (phorbol 12-myristrate 13-acetate) and with either NA, insulin or LH. Only luteal cells from days 5-10 of the cycle responded on HDL and β-mimetics (P<0.05). LH stimulated progesterone secretion from the luteal cells during all stages of the cycle (P<0.001). Cells incubated in deficient medium and supplemented with HDL secreted as much progesterone as those stimulated by LH in all stages of the cycle. Beta-mimetics were unable to enhance the stimulatory effect of HDL. Blockade of PLC had no influence on progesterone secretion from cells treated with either NA or LH, but this did impair the stimulatory effect of insulin (P<0.05). Similarly, blockade of PKC by staurosporine impaired (P<0.05) the effect of insulin only but not that observed after LH or NA treatment. We suggest that: (a) noradrenergic stimulation does not enhance utilisation of cholesterol from HDL for progesterone secretion; (b) the fasting of luteal cells seems to activate enzymes responsible for the progesterone synthesis; (c) effect of NA on progesterone secretion from luteal cells does not involve the PLC-PKC pathway.

Influence of luteinizing hormone and prostaglandin F2α on progesterone secretion in superfused bovine luteal tissue slices

1987 ◽  
Vol 116 (3) ◽  
pp. 405-412 ◽  
Author(s):  
Martina Hoedemaker ◽  
Kirsten Grunert ◽  
D. H. A. Maas ◽  
E. Grunert
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Secretion ◽  
Prostaglandin F2α ◽  
Progesterone Concentration ◽  
Tissue Slices ◽  
Progesterone Secretion ◽  
Bovine Corpus Luteum ◽  
Luteal Tissue ◽  
Prostaglandin F ◽  
Higher Doses

Abstract. Tissue slices from bovine corpus luteum from Days 12 or 13 of the oestrous cycle were super-fused for 8 h, and the progesterone secretion under the influence of prostaglandin F2α (PGF2α) and/or LH was measured. PGF2α at concentrations of 0.28 to 2800 nmol/l medium did not affect the basal progesterone secretion, whereas higher doses (7000 to 28 000 nmol/l) induced a slight increase in hormone secretion. LH, 3.4 nmol/l, caused an increase in the progesterone concentration in superfusates which exceeded the control levels (P < 0.01). This luteotropic effect of LH was not influenced by simultaneous addition of 28 to 2800 nmol/l PGF2α. PGF2α, 2800 nmol/l, did not inhibit progesterone secretion, when administered together with 0.034 to 34 nmol LH/l. Pre-superfusion with 2800 nmol/l PGF2α had no effect on the LH-stimulated increase in progesterone secretion. It is concluded that in cattle, a direct cellular effect of PGF2α, antagonizing the luteotropic function of LH, may be of less importance than other possible direct and indirect PGF2α actions.

The effect of oxytocin on progesterone secretion, phosphoinositide hydrolysis and intracellular mobilisation of Ca 2+ in porcine luteal cells

2009 ◽  
Vol 57 (1) ◽  
pp. 115-125 ◽  
Author(s):  
Anita Franczak ◽  
Beata Kurowicka ◽  
Magdalena Kowalik ◽  
Renata Ciereszko ◽  
Genowefa Kotwica
Keyword(s):  
Signal Transduction ◽  
Corpus Luteum ◽  
Inositol Phosphates ◽  
Oestrous Cycle ◽  
Steroid Secretion ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Pi Hydrolysis ◽  
Porcine Luteal Cells

Oxytocin (OT) is involved in the regulation of steroid secretion by the corpus luteum (CL) in pigs, but OT signal transduction in the porcine CL has not been identified. In this study, the effects of OT on in vitro progesterone (P 4 ) secretion, phosphoinositide (PI) hydrolysis and intracellular mobilisation of Ca 2+ ([Ca 2+ ] i ) were investigated in porcine luteal cells during the early (days 3–5), mid-(days 8–10) and late luteal phases (days 12–14) of the oestrous cycle. Basal concentrations of P 4 and accumulation of inositol phosphates (IPs) were higher (P < 0.05) on days 3–5 and 8–10 of the oestrous cycle than on days 12–14. Basal [Ca 2+ ] i mobilisation did not differ among studied periods of the oestrous cycle. Oxytocin (10 −7 M) enhanced P4 secretion and PI hydrolysis (P < 0.05) by luteal cells harvested on days 8–10 of the oestrous cycle. Moreover, OT started to increase mobilisation of [Ca 2+ ] i at the 15th (days 3–5 and 8–10) or 30th second (days 12–14) in porcine luteal cells. It was concluded that in pigs OT acts as a regulator of steroidogenesis, stimulating P 4 secretion in mature CL. This OT action may be mediated by changes in PI hydrolysis and [Ca 2+ ] i mobilisation.

Control of progesterone biosynthesis in the bovine corpus luteum: effects of luteinizing hormone and of reduced nicotinamide–adenine dinucleotide phosphate in vitro

1968 ◽  
Vol 46 (9) ◽  
pp. 1137-1145 ◽  
Author(s):  
David T. Armstrong ◽  
Donald L. Black
Keyword(s):  
Luteinizing Hormone ◽  
Corpus Luteum ◽  
Nicotinamide Adenine Dinucleotide ◽  
Specific Activity ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Stimulatory Action ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis ◽  
Reduced Nicotinamide Adenine Dinucleotide

The effects of luteinizing hormone (LH) and of reduced nicotinamide–adenine dinucleotide phosphate (NADPH) upon in vitro progesterone synthesis by bovine corpus luteum slices incubated for varying periods of time have been examined. The increased net synthesis of progesterone caused by LH was accompanied by markedly increased incorporation of acetate-1-14C into progesterone and decreased incorporation of acetate-1-14C into cholesterol. In most experiments, especially at longer incubation times, exogenous NADPH decreased incorporation of acetate-1-14C into progesterone. The effect of NADPH on incorporation of acetate-1-14C into cholesterol was variable, increasing cholesterol specific activity in some experiments and decreasing it in others. At all time intervals in all experiments, the specific activity of progesterone synthesized was greater than that of the cholesterol remaining in the tissue at the end of the interval. With increasing duration of incubations, specific activity of the progesterone synthesized became increasingly greater than that of cholesterol remaining at the end of the incubation period. These findings suggest that newly synthesized cholesterol is utilized for progesterone formation without coming into equilibrium with a large portion of the intracellular cholesterol pool. Experiments with radioactive cholesterol in vitro indicated that a significant amount of conversion of this precursor occurred in the incubation medium by enzymes which had leaked out of the tissue, and this conversion was strikingly stimulated by exogenous NADPH but not by LH. Rates of progesterone synthesis were greatest in shortest incubations, declining as incubation periods were extended. Highly significant correlations were observed between the increments, caused by LH, in net synthesis (mass) of progesterone, incorporation of acetate-1-14C into progesterone, and lactic acid production. Inhibition by puromycin of the stimulatory action of LH upon progesterone synthesis failed to prevent the stimulatory action of this gonadotropin upon glycolysis. These findings indicate that the stimulation of glycolysis is not a consequence of stimulated progesterone synthesis but may be related to the stimulatory action of LH upon 3′,5′-AMP production.

Luteotrophic factors in hyperstimulated pseudopregnant rabbit: II – High sensitivity to hCG of luteal tissue and small luteal cells

1997 ◽  
Vol 154 (2) ◽  
pp. 259-265 ◽  
Author(s):  
R K Arioua ◽  
A Benhaïm ◽  
C Féral ◽  
P Leymarie
Keyword(s):  
High Sensitivity ◽  
Chorionic Gonadotrophin ◽  
Small Cells ◽  
Corpora Lutea ◽  
Aromatase Activity ◽  
Large Contribution ◽  
Luteal Cells ◽  
Progesterone Secretion ◽  
Luteal Tissue

Abstract Previous studies on rabbit corpus luteum (CL) led to the conclusion that the luteotrophic complex, in rabbit, may include LH as well as oestradiol for normal luteal function. However, the requirement for LH is controversial. We have recently demonstrated the existence of a human chorionic gonadotrophin (hCG)-stimulated aromatase activity in cultured corpora lutea from a hyperstimulated pseudopregnant rabbit model, which develops a large number of corpora lutea, with only a few or no follicles in the ovaries. The present study was undertaken to investigate the in vitro responsiveness to hCG, dibutyryl cAMP (dbcAMP) and oestradiol of those corpora lutea. Pseudopregnancy (PP) was induced in rabbits by i.m. injection of 200 IU equine chorionic gonadotrophin daily for 2 days followed on day 4 by i.m. injection of 200 IU hCG (day 0 of PP). Luteal tissue and small and large luteal cells obtained at days 5 and 9 of PP were cultured for 24 h or during several days respectively with or without hCG, dbcAMP or oestradiol. Basal progesterone secretion was 3·6- and 22-fold higher in large cells compared with small ones at day 5 and 9 of PP respectively. When stimulated by small doses of hCG, luteal tissue responded by a 5-fold increase in progesterone secretion. Small cells produced four times higher amounts of progesterone than controls in the presence of 1 mIU/ml hCG and more than ten times in the presence of 0·1 IU/ml hCG, whereas large cells were insensitive to hCG stimulation. dbcAMP mimicked the effect of hCG on progesterone secretion by luteal tissue and luteal cells and oestradiol stimulated basal progesterone secretion in both small and large luteal cells. Given the large contribution of non stimulated large cells to luteal progesterone production and the remarkably high sensitivity of luteal tissue to gonadotrophin in vitro it seems that interactions between the two types of cells might occur during LH stimulation. Our results suggest that LH could participate in the luteotrophic complex at least in part through the stimulation of endogenous oestradiol production by luteal cells. Journal of Endocrinology (1997) 154, 259–265

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