Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants

Thomas Oelgeschläger; Robert Geiger; Thomas Rüter; Jürgen Alves; Anja Fliess; Alfred Pingoud

doi:10.1016/0378-1119(90)90201-2

Probing the function of individual amino acid residues in the DNA binding site of the EcoRI restriction endonuclease by analysing the toxicity of genetically engineered mutants

Gene ◽

10.1016/0378-1119(90)90201-2 ◽

1990 ◽

Vol 89 (1) ◽

pp. 19-27 ◽

Cited By ~ 10

Author(s):

Thomas Oelgeschläger ◽

Robert Geiger ◽

Thomas Rüter ◽

Jürgen Alves ◽

Anja Fliess ◽

...

Keyword(s):

Amino Acid ◽

Dna Binding ◽

Binding Site ◽

Restriction Endonuclease ◽

Genetically Engineered ◽

Individual Amino Acid ◽

Amino Acid Residues ◽

Dna Binding Site ◽

Ecori Restriction

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Identifying amino acid residues that contribute to the cellular-DNA binding site on retroviral integrase

Virology ◽

10.1016/j.virol.2009.04.014 ◽

2009 ◽

Vol 389 (1-2) ◽

pp. 141-148 ◽

Cited By ~ 8

Author(s):

Matthew G. Nowak ◽

Malgorzata Sudol ◽

Noelle E. Lee ◽

Wesley M. Konsavage ◽

Michael Katzman

Keyword(s):

Amino Acid ◽

Dna Binding ◽

Binding Site ◽

Amino Acid Residues ◽

Dna Binding Site ◽

Cellular Dna ◽

Retroviral Integrase

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Genetic engineering of EcoRI mutants with altered amino acid residues in the DNA binding site: physicochemical investigations give evidence for an altered monomer/dimer equilibrium for the Gln144Lys145 and Gln144Lys145Lys200 mutants

Biochemistry ◽

10.1021/bi00432a046 ◽

1989 ◽

Vol 28 (6) ◽

pp. 2667-2677 ◽

Cited By ~ 24

Author(s):

Robert Geiger ◽

Thomas Rueter ◽

Juergen Alves ◽

Anja Fliess ◽

Heiner Wolfes ◽

...

Keyword(s):

Amino Acid ◽

Dna Binding ◽

Genetic Engineering ◽

Binding Site ◽

Amino Acid Residues ◽

Dna Binding Site

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UV-catalyzed cross-linking of Escherichia coli uracil-DNA glycosylase to DNA. Identification of amino acid residues in the single-stranded DNA binding site.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)31883-5 ◽

1994 ◽

Vol 269 (34) ◽

pp. 21870-21879

Author(s):

S.E. Bennett ◽

O.N. Jensen ◽

D.F. Barofsky ◽

D.W. Mosbaugh

Keyword(s):

Escherichia Coli ◽

Amino Acid ◽

Dna Binding ◽

Binding Site ◽

Dna Glycosylase ◽

Cross Linking ◽

Amino Acid Residues ◽

Dna Identification ◽

Uracil Dna Glycosylase ◽

Dna Binding Site

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Changing the hydrogen-bonding potential in the DNA binding site of EcoRI by site-directed mutagenesis drastically reduces the enzymic activity, not, however, the preference of this restriction endonuclease for cleavage within the site -GAATTC-

Biochemistry ◽

10.1021/bi00432a047 ◽

1989 ◽

Vol 28 (6) ◽

pp. 2678-2684 ◽

Cited By ~ 57

Author(s):

Jurgen Alves ◽

Thomas Rueter ◽

Robert Geiger ◽

Anja Fliess ◽

Guenter Maass ◽

...

Keyword(s):

Hydrogen Bonding ◽

Dna Binding ◽

Binding Site ◽

Restriction Endonuclease ◽

Enzymic Activity ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Dna Binding Site ◽

Hydrogen Bonding Potential

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Mutational Analysis of the Function of Gln115 in the EcoRI Restriction Endonuclease, a Critical Amino Acid for Recognition of the Inner Thymidine Residue in the Sequence -GAATTC- and for Coupling Specific DNA Binding to Catalysis

Journal of Molecular Biology ◽

10.1006/jmbi.1993.1019 ◽

1993 ◽

Vol 229 (1) ◽

pp. 221-234 ◽

Cited By ~ 22

Author(s):

Albert Jeltsch ◽

Jürgen Alves ◽

Thomas Oelgeschläger ◽

Heiner Wolfes ◽

Günter Maass ◽

...

Keyword(s):

Amino Acid ◽

Dna Binding ◽

Restriction Endonuclease ◽

Mutational Analysis ◽

Critical Amino Acid ◽

Ecori Restriction

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Crosslinking the EcoRV restriction endonuclease across the DNA-binding site reveals transient intermediates and conformational changes of the enzyme during DNA binding and catalytic turnover

The EMBO Journal ◽

10.1093/emboj/17.22.6757 ◽

1998 ◽

Vol 17 (22) ◽

pp. 6757-6766 ◽

Cited By ~ 13

Author(s):

C. Schulze

Keyword(s):

Dna Binding ◽

Binding Site ◽

Restriction Endonuclease ◽

Conformational Changes ◽

Dna Binding Site ◽

Transient Intermediates

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Determination of the Neurospora crassa CYS 3 sulfur regulatory protein consensus DNA-binding site: amino-acid substitutions in the CYS3 bZIP domain that alter DNA-binding specificity

Current Genetics ◽

10.1007/s002940050136 ◽

1996 ◽

Vol 30 (4) ◽

pp. 298-304 ◽

Cited By ~ 19

Author(s):

Qunhui Li ◽

G. A. Marzluf

Keyword(s):

Amino Acid ◽

Dna Binding ◽

Neurospora Crassa ◽

Binding Site ◽

Regulatory Protein ◽

Amino Acid Substitutions ◽

Dna Binding Specificity ◽

Dna Binding Site ◽

Bzip Domain

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Faculty Opinions recommendation of DNA binding site sequence directs glucocorticoid receptor structure and activity.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1160087.623811 ◽

2009 ◽

Author(s):

Robert Sapolsky

Keyword(s):

Dna Binding ◽

Glucocorticoid Receptor ◽

Binding Site ◽

Receptor Structure ◽

Dna Binding Site ◽

Structure And Activity

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Faculty Opinions recommendation of Simulations of DNA topoisomerase 1B bound to supercoiled DNA reveal changes in the flexibility pattern of the enzyme and a secondary protein-DNA binding site.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718505331.793500084 ◽

2014 ◽

Author(s):

Anthony Maxwell

Keyword(s):

Dna Binding ◽

Binding Site ◽

Dna Topoisomerase ◽

Supercoiled Dna ◽

Dna Binding Site

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DNA Binding Drugs Targeting the Regulatory DNA Binding Site of the ETS Domain Family Transcription Factor Associated With Human Breast Cancer

10.21236/ada381309 ◽

1999 ◽

Author(s):

Yong-Dong Wang

Keyword(s):

Breast Cancer ◽

Transcription Factor ◽

Dna Binding ◽

Binding Site ◽

Human Breast Cancer ◽

Human Breast ◽

Domain Family ◽

Dna Binding Site ◽

Ets Domain ◽

Regulatory Dna

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