Cloning and characterization of the gene encoding an extracellular alkaline serine protease from Vibrio metschnikovii strain RH530

Gene ◽  
1995 ◽  
Vol 152 (1) ◽  
pp. 59-63 ◽  
Author(s):  
Yong Tae Kwon ◽  
Jin Oh Kim ◽  
Sun Young Moon ◽  
Young Dong Yoo ◽  
Hyune Mo Rho
Keyword(s):  
Serine Protease ◽  
Gene Encoding ◽  
Alkaline Serine Protease ◽  
Vibrio Metschnikovii

scholarly journals Purification and Biochemical Characterization of Alkaline Serine Protease from Caesalpinia bonducella

2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500
Author(s):  
Hidayatullah Khan ◽  
Irshad Ali ◽  
Arif-ullah Khan ◽  
Mushtaq Ahmed ◽  
Zamarud Shah ◽  
...  
Keyword(s):  
Serine Protease ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Sodium Dodecyl ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
Alkaline Serine Protease ◽  
Protease Enzyme ◽  
Caesalpinia Bonducella

A high molecular weight serine protease has been purified to electrophoretic homogeneity from the seeds of Caesalpinia bonducella Flem. (Caesalpiniaceae) by the combination of size exclusion and ion exchange chromatography. About 524 fold purification was achieved with an overall recovery of 6.8%. The specific activity was found to be 86 U/mg/min at pH 8.0. The calculated Km and Vmax were 1.66 mg/mL and 496.68 units/min per mg of protein, respectively. The molecular mass was estimated to be about 63 kDa by sodium dodecyl sulfate PAGE. The enzyme showed optimum activity at pH 8.0 and exhibited its highest activity at 40°C. The enzyme was strongly inhibited by 2mM phenylmethylsulfonyl fluoride (PMSF), suggesting the presence of a serine residue at the active site. PMSF showed a pure competitive type of inhibition with the serine protease enzyme. It was observed that enzyme activity was enhanced in the presence of dications and was active against a variety of modified substrates and natural proteins.

Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1

2009 ◽  
Vol 36 (7) ◽  
pp. 939-948 ◽  
Author(s):  
Kemel Jellouli ◽  
Ali Bougatef ◽  
Laila Manni ◽  
Rym Agrebi ◽  
Rayda Siala ◽  
...  
Keyword(s):  
Serine Protease ◽  
Biochemical Characterization ◽  
Vibrio Metschnikovii ◽  
Extracellular Serine Protease
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