Determination of 7α-hydroxy-4-cholesten-3-one level in plasma using isotope-dilution mass spectrometry and monitoring its circadian rhythm in human as an index of bile acid biosynthesis

1994 ◽  
Vol 655 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Tadashi Yoshida ◽  
Akira Honda ◽  
Naomi Tanaka ◽  
Yasushi Matsuzaki ◽  
Junichi Shoda ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Circadian Rhythm ◽  
Bile Acid ◽  
Isotope Dilution ◽  
Isotope Dilution Mass Spectrometry ◽  
Acid Biosynthesis ◽  
Bile Acid Biosynthesis

scholarly journals Determination of Key Intermediates in Cholesterol and Bile Acid Biosynthesis by Stable Isotope Dilution Mass Spectrometry

2008 ◽  
Vol 3 ◽  
pp. ACI.S611 ◽  
Author(s):  
Tadashi Yoshida ◽  
Akira Honda ◽  
Hiroshi Miyazaki ◽  
Yasushi Matsuzaki
Keyword(s):  
Mass Spectrometry ◽  
Bile Acid ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
High Performance ◽  
Isotope Dilution Mass Spectrometry ◽  
Acid Biosynthesis ◽  
Bile Acid Biosynthesis ◽  
Stable Isotope Dilution

For more than a decade, we have developed stable isotope dilution mass spectrometry methods to quantify key intermediates in cholesterol and bile acid biosynthesis, mevalonate and oxysterols, respectively. The methods are more sensitive and reproducible than conventional radioisotope (RI), gas-chromatography (GC) or high-performance liquid chromatography (HPLC) methods, so that they are applicable not only to samples from experimental animals but also to small amounts of human specimens. In this paper, we review the development of stable isotope dilution mass spectrometry for quantifying mevalonate and oxysterols in biological materials, and demonstrate the usefulness of this technique.

Determination of cyanuric acid using exact matching isotope dilution mass spectrometry

2013 ◽  
Vol 36 (6) ◽  
pp. 1054-1060
Author(s):  
Guiwan Koh ◽  
Ee-mei Gui ◽  
Tang-Lin Teo ◽  
Tong-Kooi Lee
Keyword(s):  
Mass Spectrometry ◽  
Isotope Dilution ◽  
Cyanuric Acid ◽  
Isotope Dilution Mass Spectrometry ◽  
Exact Matching
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