Proton NMR analysis of plasma from renal failure patients: Evaluation of sample preparation and spectral-editing methods

The stereoisomers of 2-methyltetrahydropyran-4-ol have been separated and identified by carbon-13 and proton nmr analysis of the trideuteriomethyl-2,6,6-trideuterio analogue. Stereoisomeric product ratios of reduction of 2-methyltetrahydropyran-4-one (1) by NaBH4, KBH4, L-Selectride, K-Selectride, and LiBH(nBu)3 have been determined and compared with reductions of 3-methylcyclohexanone. Product ratios in the reduction of the two substrates by the borohydride reducing agents are similar but are quite different in the reduction by the Selectride reducing agents, 1 being reduced by Selectride to give 73% equatorialalcohol. Two possible mechanisms of reduction of 1 are proposed, involving intramolecular assistance by the cyclic ether oxygen.

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NMR analysis of budding yeast metabolomics: a rapid method for sample preparation

Molecular BioSystems ◽

10.1039/c4mb00452c ◽

2015 ◽

Vol 11 (2) ◽

pp. 379-383 ◽

Cited By ~ 14

Author(s):

C. Airoldi ◽

F. Tripodi ◽

C. Guzzi ◽

R. Nicastro ◽

P. Coccetti

Keyword(s):

Nmr Spectroscopy ◽

Sample Preparation ◽

Rapid Method ◽

Budding Yeast ◽

Yeast Cells ◽

Intracellular Metabolite ◽

Nmr Analysis ◽

H Nmr ◽

Metabolite Extraction

We present a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells analyzed by1H-NMR spectroscopy.

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Proton NMR analysis of plasma is a weak predictor of coronary artery disease

Nature Medicine ◽

10.1038/nm1432 ◽

2006 ◽

Vol 12 (6) ◽

pp. 705-710 ◽

Cited By ~ 133

Author(s):

Heide L Kirschenlohr ◽

Julian L Griffin ◽

Sarah C Clarke ◽

Ranyl Rhydwen ◽

Andrew A Grace ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Proton Nmr ◽

Nmr Analysis ◽

Artery Disease

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Carbon-13 and proton NMR analysis of isotopically labeled benzo[a]pyrenes

Journal of the American Chemical Society ◽

10.1021/ja00342a010 ◽

1983 ◽

Vol 105 (4) ◽

pp. 733-735 ◽

Cited By ~ 28

Author(s):

Clifford J. Unkefer ◽

Robert E. London ◽

Thomas W. Whaley ◽

Guido H. Daub

Keyword(s):

Proton Nmr ◽

Nmr Analysis

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Application for proton NMR spectral editing techniques for selective observation of nitrogen-hydrogen (N-H) protons in an actinomycin D complex with a tetranucleotide duplex

Journal of the American Chemical Society ◽

10.1021/ja00356a054 ◽

1983 ◽

Vol 105 (18) ◽

pp. 5945-5946 ◽

Cited By ~ 6

Author(s):

David G. Reid ◽

David M. Doddrell ◽

Keith R. Fox ◽

Stephen A. Salisbury ◽

Dudley H. Williams

Keyword(s):

Actinomycin D ◽

Proton Nmr ◽

Spectral Editing

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A Quantitative NMR Method for Silyllithium Analysis

Synlett ◽

10.1055/s-0036-1590902 ◽

2017 ◽

Vol 28 (18) ◽

pp. 2449-2452

Author(s):

Scott Sieburth ◽

Yingjian Bo

Keyword(s):

Proton Nmr ◽

Relative Quantification ◽

Nmr Analysis ◽

Quantitative Nmr ◽

Simple Method ◽

Nmr Method

A rapid and extremely simple method for silyl anion analysis is presented. The progress of silyllithium reagent preparation can be determined by quenching an aliquot with neat chloro(trimethyl)silane, evaporation, dilution with CDCl3, and direct proton NMR analysis. This procedure is fast, simple, and allows for identification and relative quantification of the starting reagent, intermediates, and the silyllithium product.

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