GROWTH CONE BEHAVIOR IN THE PRESENCE OF SOLUBLE CHONDROITIN SULFATE PROTEOGLYCAN (CSPG), COMPARED TO BEHAVIOR ON CSPG BOUND TO LAMININ OR FIBRONECTIN

1996 ◽  
Vol 14 (3) ◽  
pp. 331-349 ◽  
Author(s):  
Diane M. Snow ◽  
Eric M. Brown ◽  
Paul C. Letourneau
Keyword(s):  
Chondroitin Sulfate ◽  
Growth Cone ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan

Actin filament bundles are required for microtubule reorientation during growth cone turning to avoid an inhibitory guidance cue

1996 ◽  
Vol 109 (8) ◽  
pp. 2031-2040 ◽  
Author(s):  
J.F. Challacombe ◽  
D.M. Snow ◽  
P.C. Letourneau
Keyword(s):  
Chondroitin Sulfate ◽  
Actin Filament ◽  
Growth Cone ◽  
Actin Filaments ◽  
Cytochalasin B ◽  
Growth Cones ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Filament Bundles ◽  
Microtubule Reorientation

The extracellular matrix through which growth cones navigate contains molecules, such as chondroitin sulfate proteoglycan, that can inhibit growth cone advance and induce branching and turning. Growth cone turning is accompanied by rearrangement of the cytoskeleton. To identify changes in the organization of actin filaments and microtubules that occur as growth cones turn, we used time-lapse phase contrast videomicroscopy to observe embryonic chick dorsal root ganglion neuronal growth cones at a substratum border between fibronectin and chondroitin sulfate proteoglycan, in the presence and absence of cytochalasin B. Growth cones were fixed and immunocytochemically labeled to identify actin filaments and dynamic and stable microtubules. Our results suggest that microtubules are rearranged within growth cones to accomplish turning to avoid chondroitin sulfate proteoglycan. Compared to growth cones migrating on fibronectin, turning growth cones were more narrow, and they contained dynamic microtubules that were closer to the leading edge and were more bundled. Cytochalasin B-treated growth cones sidestepped laterally along the border instead of turning, and in sidestepping growth cones, microtubules were not bundled and aligned. We conclude that actin filament bundles are required for microtubule reorientation and growth cone turning to avoid chondroitin sulfate proteoglycan.

scholarly journals Biosynthesis of Chondroitin Sulfate Proteoglycan

1972 ◽  
Vol 247 (12) ◽  
pp. 3838-3847
Author(s):  
John R. Baker ◽  
Lennart Rodén ◽  
Allen C. Stoolmiller
Keyword(s):  
Chondroitin Sulfate ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan

scholarly journals Brevican, a Chondroitin Sulfate Proteoglycan of Rat Brain, Occurs as Secreted and Cell Surface Glycosylphosphatidylinositol-anchored Isoforms

1995 ◽  
Vol 270 (45) ◽  
pp. 27206-27212 ◽  
Author(s):  
Constanze I. Seidenbecher ◽  
Karin Richter ◽  
Uwe Rauch ◽  
Reinhard Fässler ◽  
Craig C. Garner ◽  
...  
Keyword(s):  
Cell Surface ◽  
Chondroitin Sulfate ◽  
Rat Brain ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan
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