IgG-subclass-specific antibody reactivity to respiratory syncytial virus polypeptides investigated by Western blot

1990 ◽  
Vol 141 (3) ◽  
pp. 343-353 ◽  
Author(s):  
M Jankowski ◽  
A Hornsleth ◽  
P.G Olsen
Keyword(s):  
Respiratory Syncytial Virus ◽  
Western Blot ◽  
Specific Antibody ◽  
Igg Subclass ◽  
Antibody Reactivity ◽  
Syncytial Virus

Safety and Immunogenicity of a Respiratory Syncytial Virus Fusion (F) Protein Nanoparticle Vaccine in Healthy Third-Trimester Pregnant Women and Their Infants

2019 ◽  
Vol 220 (11) ◽  
pp. 1802-1815 ◽  
Author(s):  
Flor M Muňoz ◽  
Geeta K Swamy ◽  
Somia P Hickman ◽  
Sapeckshita Agrawal ◽  
Pedro A Piedra ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Pregnant Women ◽  
Specific Antibody ◽  
Post Partum ◽  
Study Groups ◽  
Third Trimester ◽  
F Protein ◽  
Before And After ◽  
Syncytial Virus ◽  
Tract Disease

Abstract Background Respiratory syncytial virus (RSV) is the leading cause of infant lower respiratory tract disease and hospitalization worldwide. Methods Safety and immunogenicity of RSV fusion (F) protein nanoparticle vaccine or placebo were evaluated in 50 healthy third-trimester pregnant women. Assessments included vaccine tolerability and safety in women and infants, and RSV-specific antibody measures in women before and after vaccination, at delivery and post partum. Results The vaccine was well tolerated; no meaningful differences in pregnancy or infant outcomes were observed between study groups. RSV-specific antibody levels increased significantly among vaccine recipients, including responses competitive with well-described monoclonal antibodies specific for multiple RSV neutralizing epitopes. No significant antibody increase was seen among placebo recipients, although a shallow upward trend across the RSV season was noted. Transplacental antibody transfer was 90%–120% across assays for infants of vaccinated women. Women with an interval of ≥30 days between vaccination and delivery demonstrated higher placental antibody transfer rates than women with an interval <30 days. Half-lives of RSV-specific antibodies in infants approximated 40 days. There was no evidence of severe RSV disease in infants of vaccinated mothers. Conclusions Data from this phase 2 study support a maternal immunization strategy to protect infants from RSV disease. Clinical Trials Registration NCT02247726.

scholarly journals Shedding course of bovine respiratory syncytial virus and bovine parainfluenza 3 virus in calves vaccinated intranasally

2013 ◽  
Vol 57 (4) ◽  
pp. 479-483 ◽  
Author(s):  
Wojciech Socha ◽  
Jerzy Rola ◽  
Dariusz Bednarek ◽  
Renata Urban-Chmiel ◽  
Jan F. Żmudziński
Keyword(s):  
Respiratory Syncytial Virus ◽  
Specific Antibody ◽  
Bovine Respiratory Syncytial Virus ◽  
Parainfluenza Virus ◽  
Rt Pcr ◽  
Nasal Swabs ◽  
Before And After ◽  
Antibody Titres ◽  
Syncytial Virus ◽  
Bovine Parainfluenza Virus

Abstract Shedding time of bovine respiratory syncytial virus (BRSV) and bovine parainfluenza virus 3 (BPIV3) in calves vaccinated intranasally with modified live Rispoval RS-PI3 vaccine was determined. Blood and nasal swabs were collected on selected days before and after vaccination. Antibodies against BRSV and BPIV3 were tested by Respiratory ELISA Pentakit and the viral RNA was detected by RT-PCR. Twenty eight days after administration of the vaccine, a marked increase of specific antibody titres to BRSV and BPIV3 was detected in vaccinated calves. All animals were RT-PCR positive both for BRSV and BPIV3. Both viruses were excreted with nasal discharges within 8 d after vaccination but the course of shedding in individual calves was variable.

Immunologic Response of Adenoidal Lymphocytes to Respiratory Syncytial Virus

1992 ◽  
Vol 101 (10) ◽  
pp. 848-853 ◽  
Author(s):  
Nobuo Soh ◽  
David Nadal ◽  
Joel M. Bernstein ◽  
Erika Schläpfer ◽  
Pearay L. Ogra
Keyword(s):  
Respiratory Syncytial Virus ◽  
Specific Antibody ◽  
Lymphocyte Culture ◽  
Pokeweed Mitogen ◽  
Antibody Activity ◽  
Antibody Synthesis ◽  
Viral Pathogens ◽  
Specific Igg ◽  
Syncytial Virus

Adenoidal lymphocytes obtained from 43 subjects with serum antibodies to respiratory syncytial virus (RSV) were established in culture in vitro and analyzed for immunoglobulin (Ig) and RSV-specific antibody synthesis. Spontaneous synthesis of Ig was consistently observed in culture supernatants. The ratios of IgA to IgG and IgM to IgG in adenoidal lymphocyte culture supernatant were higher than in serum. In cell cultures stimulated with RSV or pokeweed mitogen, RSV antibody activity was detected in 25 of 43 (58.1%) for IgG, 5 of 43 (11.6%) for IgA, and 4 of 43 (9.3%) for IgM. Also, RSV-specific IgG was detected in some supernatants from unstimulated cultures. In seven cases the cultures of autologous tonsillar lymphocytes were also investigated. Autologous organs exhibited similar polyclonal Ig production and RSV-specific antibody synthesis. These observations demonstrate that both adenoids and palatine tonsils are continuously engaged in synthesis of local antibodies to viral pathogens available to the nasopharynx and respiratory mucosa.

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