The effects of human calcitonin on the cytoplasmic spreading of rat osteoclasts

Bone ◽  
1986 ◽  
Vol 7 (4) ◽  
pp. 308-308
Author(s):  
J.A. Darby ◽  
J.C. Chambers ◽  
T.J. Chambers
Keyword(s):  
1973 ◽  
Vol 71 (4_Suppl) ◽  
pp. S161 ◽  
Author(s):  
R.-D. Hesch ◽  
J. S. Woodhead ◽  
M. Hüfner ◽  
F. M. Dietrich
Keyword(s):  

Nitric Oxide ◽  
2020 ◽  
Vol 104-105 ◽  
pp. 11-19
Author(s):  
Huixian Ye ◽  
Hailing Li ◽  
Zhonghong Gao

1985 ◽  
Vol 85 (1) ◽  
pp. 33-48 ◽  
Author(s):  
Hiroyuki YAMAMOTO ◽  
Masanobu OZAKI ◽  
Shiroh KISHIOKA ◽  
Yoshiyuki IGUCHI ◽  
Sadako TAMURA

2008 ◽  
Vol 19 (8) ◽  
pp. 1596-1603 ◽  
Author(s):  
I. Neundorf ◽  
R. Rennert ◽  
J. Franke ◽  
I. Közle ◽  
R. Bergmann

2004 ◽  
Vol 382 (3) ◽  
pp. 945-956 ◽  
Author(s):  
Rachel TRÉHIN ◽  
Hanne M. NIELSEN ◽  
Heinz-Georg JAHNKE ◽  
Ulrike KRAUSS ◽  
Annette G. BECK-SICKINGER ◽  
...  

We assessed the metabolic degradation kinetics and cleavage patterns of some selected CPP (cell-penetrating peptides) after incubation with confluent epithelial models. Synthesis of N-terminal CF [5(6)-carboxyfluorescein]-labelled CPP, namely hCT (human calcitonin)-derived sequences, Tat(47–57) and penetratin(43–58), was through Fmoc (fluoren-9-ylmethoxycarbonyl) chemistry. Metabolic degradation kinetics of the tested CPP in contact with three cell-cultured epithelial models, MDCK (Madin–Darby canine kidney), Calu-3 and TR146, was evaluated by reversed-phase HPLC. Identification of the resulting metabolites of CF-hCT(9–32) was through reversed-phase HPLC fractionation and peak allocation by MALDI–TOF-MS (matrix-assisted laser-desorption ionization–time-of-flight mass spectrometry) or direct MALDI–TOF-MS of incubates. Levels of proteolytic activity varied highly between the investigated epithelial models and the CPP. The Calu-3 model exhibited the highest proteolytic activity. The patterns of metabolic cleavage of hCT(9–32) were similar in all three models. Initial cleavage of this peptide occurred at the N-terminal domain, possibly by endopeptidase activity yielding both the N- and the C-terminal counterparts. Further metabolic degradation was by aminopeptidase, endopeptidase and/or carboxypeptidase activities. In conclusion, when in contact with epithelial models, the studied CPP were subject to efficient metabolism, a prerequisite of cargo release on the one hand, but with potential for premature cleavage and loss of the cargo as well on the other. The results, particularly on hCT(9–32), may be used as a template to suggest structural modifications towards improved CPP performance.


1989 ◽  
Vol 256 (2) ◽  
pp. E331-E335 ◽  
Author(s):  
T. Chiba ◽  
A. Yamaguchi ◽  
T. Yamatani ◽  
A. Nakamura ◽  
T. Morishita ◽  
...  

From this study, we predicted that the human calcitonin gene-related peptide (hCGRP) fragment hCGRP-(8-37) would be a selective antagonist for CGRP receptors but an agonist for calcitonin (CT) receptors. In rat liver plasma membrane, where CGRP receptors predominate and CT appears to act through these receptors, hCGRP-(8-37) dose dependently displaced 125I-[Tyr0]rat CGRP binding. However, hCGRP-(8-37) had no effect on adenylate cyclase activity in liver plasma membrane. Furthermore, hCGRP-(8-37) inhibited adenylate cyclase activation induced not only by hCGRP but also by hCT. On the other hand, in LLC-PK1 cells, where calcitonin receptors are abundant and CGRP appears to act via these receptors, the bindings of 125I-[Tyr0]rat CGRP and 125I-hCT were both inhibited by hCGRP-(8-37). In contrast to liver membranes, interaction of hCGRP-(8-37) with these receptors led to stimulation of adenosine 3',5'-cyclic monophosphate (cAMP) production in LLC-PK1 cells, and moreover, this fragment did not inhibit the increased production of cAMP induced not only by hCT but also by hCGRP. Thus hCGRP-(8-37) appears to be a useful tool for determining whether the action of CGRP as well as that of CT is mediated via specific CGRP receptors or CT receptors.


2005 ◽  
Vol 12 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Kiyoshi Ogawa ◽  
Shigenori Nishimura ◽  
Masamitsu Doi ◽  
Hiroyuki Takashima ◽  
Yoshinori Nishi ◽  
...  

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