Isolation and characterization of a metalloprotease associated with chicken epiphyseal cartilage matrix vesicles

Bone ◽  
1986 ◽  
Vol 7 (2) ◽  
pp. 137-143 ◽  
Author(s):  
N. Katsura ◽  
K. Yamada
Keyword(s):  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Epiphyseal Cartilage ◽  
Isolation And Characterization

Effects of EDTA, Hyaluronidase and Collagenase on Epiphyseal Cartilage Matrix

1968 ◽  
Vol 26 ◽  
pp. 56-57
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter
Keyword(s):  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Dense Matrix ◽  
Type Iv ◽  
Bovine Testicular Hyaluronidase ◽  
The Matrix ◽  
Testicular Hyaluronidase

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.

scholarly journals Isolation and characterization of a 35,000 molecular weight subunit fetal cartilage matrix protein.

1983 ◽  
Vol 258 (1) ◽  
pp. 655-661 ◽  
Author(s):  
H U Choi ◽  
L H Tang ◽  
T L Johnson ◽  
S Pal ◽  
L C Rosenberg ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Matrix Protein ◽  
Cartilage Matrix ◽  
Isolation And Characterization

scholarly journals Characterization of the nucleational core complex responsible for mineral induction by growth plate cartilage matrix vesicles.

1993 ◽  
Vol 268 (33) ◽  
pp. 25084-25094
Author(s):  
L N Wu ◽  
T Yoshimori ◽  
B R Genge ◽  
G R Sauer ◽  
T Kirsch ◽  
...  
Keyword(s):  
Growth Plate ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Core Complex ◽  
Growth Plate Cartilage

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document