scholarly journals Quantitative Ratiometric Imaging of FRET-Biosensors in Living Cells

2013 ◽  
pp. 593-609 ◽  
Author(s):  
Désirée Spiering ◽  
Jose Javier Bravo-Cordero ◽  
Yasmin Moshfegh ◽  
Veronika Miskolci ◽  
Louis Hodgson
Keyword(s):  
Living Cells ◽  
Fret Biosensors ◽  
Ratiometric Imaging

Two-photon fluorescent Zn2+ probe for ratiometric imaging and biosensing of Zn2+ in living cells and larval zebrafish

2020 ◽  
Vol 148 ◽  
pp. 111666 ◽  
Author(s):  
Wanying Li ◽  
Zhichao Liu ◽  
Bingqing Fang ◽  
Ming Jin ◽  
Yang Tian
Keyword(s):  
Living Cells ◽  
Two Photon ◽  
Larval Zebrafish ◽  
Ratiometric Imaging

scholarly journals FRET Microscopy in Yeast

Biosensors ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 122 ◽  
Author(s):  
Skruzny ◽  
Pohl ◽  
Abella
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fluorescent Proteins ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Bioactive Molecules ◽  
Biophysical Processes ◽  
Microscopy Method ◽  
Fret Biosensors

Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts.

scholarly journals DNAzyme‐Mediated Genetically Encoded Sensors for Ratiometric Imaging of Metal Ions in Living Cells

2019 ◽  
Vol 132 (5) ◽  
pp. 1907-1912
Author(s):  
Mengyi Xiong ◽  
Zhenglin Yang ◽  
Ryan J. Lake ◽  
Junjie Li ◽  
Shanni Hong ◽  
...  
Keyword(s):  
Metal Ions ◽  
Living Cells ◽  
Ratiometric Imaging ◽  
Genetically Encoded Sensors

Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

2017 ◽  
Vol 8 (37) ◽  
pp. 5795-5802 ◽  
Author(s):  
Hong Wang ◽  
Peisheng Zhang ◽  
Yongxiang Hong ◽  
Bin Zhao ◽  
Pinggui Yi ◽  
...  
Keyword(s):  
Membrane Permeability ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Polymer Dots ◽  
Ratiometric Imaging ◽  
Fluorescent Polymer

FRET-based fluorescent polymer dots (FPD) with good membrane permeability have been developed for ratiometric imaging of lysosomal HClO in living cells.

A water-soluble two-photon fluorescence chemosensor for ratiometric imaging of mitochondrial viscosity in living cells

2016 ◽  
Vol 4 (35) ◽  
pp. 5907-5912 ◽  
Author(s):  
Meng Zhao ◽  
Yingzhong Zhu ◽  
Jian Su ◽  
Qian Geng ◽  
Xiaohe Tian ◽  
...  
Keyword(s):  
Living Cells ◽  
Water Soluble ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We report a novel water-soluble ratiometric TPEF chemosensor EIN that is specifically responsive and singularly sensitive to mitochondria viscosity in living cells.

Development of FRET Biosensors to Detect Kinase Activity in Living Cells

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Jennifer Okáľová ◽  
Jeremy Burton ◽  
Neil J. Grimsey
Keyword(s):  
Kinase Activity ◽  
Living Cells ◽  
Fret Biosensors

A Fluorescent Probe for Ratiometric Imaging of SO2 Derivatives in Mitochondria of Living Cells

2016 ◽  
Vol 55 (6) ◽  
pp. 1477-1483 ◽  
Author(s):  
Haidong Li ◽  
Qichao Yao ◽  
Jiangli Fan ◽  
Chong Hu ◽  
Feng Xu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Ratiometric Imaging

scholarly journals Engineering of a zero cross-talk fluorescent polymer nanoprobe for self-referenced ratiometric imaging of lysosomal hypochlorous acid in living cells

2020 ◽  
Vol 4 (3) ◽  
pp. 862-868 ◽  
Author(s):  
Hongqing Wei ◽  
Rongjin Zeng ◽  
Shenglan Wang ◽  
Chong-Hua Zhang ◽  
Shu Chen ◽  
...  
Keyword(s):  
Cross Talk ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Live Cells ◽  
Ratiometric Imaging ◽  
Fluorescent Polymer

A zero cross-talk fluorescent polymer nanoprobe with a self-referenced ratiometric effect was rationally designed and synthesized for lysosomal hypochlorous acid (HClO) imaging in live cells.

A nucleus targetable fluorescent probe for ratiometric imaging of endogenous NO in living cells and zebrafishes

The Analyst ◽  
2021 ◽  
Author(s):  
Liang Zhao ◽  
Zhenlong Huang ◽  
Daqing Ma ◽  
Yu Yan ◽  
Xinfu Zhang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Live Cells ◽  
Real Time Monitoring ◽  
Immune Systems ◽  
Ratiometric Imaging ◽  
Messenger Molecule

Nitric oxide (NO) is an important cellular messenger molecule in the cardiovascular, nervous and immune systems. Real-time monitoring of NO activity in specific organelles of live cells is important to...

Sign in / Sign up

Export Citation Format

Share Document