Sanger Dideoxy Sequencing of DNA

ABSTRACT Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

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Problem-solving test: Dideoxy sequencing

Biochemistry and Molecular Biology Education ◽

10.1002/bmb.20387 ◽

2010 ◽

Vol 38 (2) ◽

pp. 117-119 ◽

Cited By ~ 1

Author(s):

József Szeberényi

Keyword(s):

Problem Solving ◽

Dideoxy Sequencing

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Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1

DNA Sequencing Protocols ◽

10.1385/0-89603-248-5:83 ◽

2003 ◽

pp. 83-90

Author(s):

Alan T. Bankier

Keyword(s):

Dna Polymerase ◽

Klenow Fragment ◽

Dideoxy Sequencing

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An efficient synthetic primer for the M13 cloning dideoxy sequencing system

Bioscience Reports ◽

10.1007/bf01114830 ◽

1982 ◽

Vol 2 (9) ◽

pp. 687-696 ◽

Cited By ~ 10

Author(s):

Peter C. K. Lau ◽

John H. Spencer

Keyword(s):

Dideoxy Sequencing

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Dideoxy sequencing

The Dictionary of Genomics, Transcriptomics and Proteomics ◽

10.1002/9783527678679.dg03036 ◽

2015 ◽

pp. 1-1

Keyword(s):

Dideoxy Sequencing

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Sequencing of large double-stranded DNA using the dideoxy sequencing technique

Bioscience Reports ◽

10.1007/bf01114897 ◽

1982 ◽

Vol 2 (11) ◽

pp. 907-912 ◽

Cited By ~ 19

Author(s):

G. F. Hong

Keyword(s):

Sequence Analysis ◽

Direct Sequencing ◽

Dna Molecules ◽

Coding Region ◽

Base Pairs ◽

Dideoxy Sequencing ◽

Double Stranded Dna ◽

Sequencing Technique ◽

Eukaryotic Dna

The dideoxy sequencing technique has been applied to the direct sequencing of large double-stranded DNA molecules with a small single-stranded primer. For instance, the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure proves useful in the sequence analysis of a large number of different mutations in a particular region and in the analysis of eukaryotic DNA cloned in plasmids, phages, and cosmids.

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