REGULATION OF GENE EXPRESSION IN TRANSGENIC TOMATO PLANTS BY ANTISENSE RNA AND RIPENING-SPECIFIC PROMOTERS

Author(s):  
D. GRIERSON ◽  
C.J.S. SMITH ◽  
C.F. WATSON ◽  
P.C. MORRIS ◽  
J.E. GRAY ◽  
...  
2013 ◽  
Vol 48 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Mihail Kantor ◽  
Radu Sestras ◽  
Kamal Chowdhury

The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.


2001 ◽  
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pp. 427-433 ◽  
Author(s):  
Sergei F. Krasnyanski ◽  
Jagdeep Sandhu ◽  
Leslie L. Domier ◽  
Dennis E. Buetow ◽  
Schuyler S. Korban

2014 ◽  
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pp. 14-17 ◽  
Author(s):  
Ye. N. Baranova ◽  
E. N. Akanov ◽  
A. A. Gulevich ◽  
L. V. Kurenina ◽  
S. A. Danilova ◽  
...  

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Vol 218 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Michael W. Lassner ◽  
Joseph M. Palys ◽  
John I. Yoder

2019 ◽  
Vol 135 ◽  
pp. 77-86 ◽  
Author(s):  
Mourad Baghour ◽  
Francisco Javier Gálvez ◽  
M. Elena Sánchez ◽  
M. Nieves Aranda ◽  
Kees Venema ◽  
...  

1991 ◽  
Vol 3 (11) ◽  
pp. 1187 ◽  
Author(s):  
Harry J. Klee ◽  
Maria B. Hayford ◽  
Keith A. Kretzmer ◽  
Gerard F. Barry ◽  
Ganesh M. Kishore

2009 ◽  
Vol 35 (4) ◽  
pp. 223-226 ◽  
Author(s):  
E. K. Serenko ◽  
V. N. Ovchinnikova ◽  
L. V. Kurenina ◽  
E. N. Baranova ◽  
A. A. Gulevich ◽  
...  

1992 ◽  
Vol 20 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Caius M. T. Rommens ◽  
George N. Rudenko ◽  
Paul P. Dijkwel ◽  
Mark J. J. van Haaren ◽  
Pieter B. F. Ouwerkerk ◽  
...  

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