Genomics of Odor Receptors in Zebrafish

In biological olfactory systems, odor receptors receive odor molecules by recognizing the molecular information. Humans can sense the odor by the signal from these activated receptors. The combination of the activated receptors is called “odor code,” and the odor codes are expressed as an “odor cluster map” of glomeruli on the olfactory bulb surface. The odor code is essential information for qualitative and quantitative analyses of odor sensation. In this chapter, development of odor sensors based on the odor code concept and an attempt to extract the parameters for odor coding from molecular informatics are described. Application of the obtained odor code for odor reproduction is also presented.

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Complex and non-redundant signals from individual odor receptors that underlie chemotaxis behavior in Drosophila melanogaster larvae

Biology Open ◽

10.1242/bio.20148573 ◽

2014 ◽

Vol 3 (10) ◽

pp. 947-957 ◽

Cited By ~ 3

Author(s):

J. S. Grewal ◽

C. Nguyen ◽

R. Robles ◽

C. Cho ◽

K. Kir ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Individual Odor ◽

Odor Receptors

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The feasibility study of novel odor biosensor using dissociate neuronal culture expressing ion channel built-in odor receptors

Neuroscience Research ◽

10.1016/j.neures.2010.07.199 ◽

2010 ◽

Vol 68 ◽

pp. e98

Author(s):

Norio Tanada ◽

Takeshi Sakurai ◽

Hidefumi Mitsuno ◽

Bakkum Douglas ◽

Ryohei Kanzaki ◽

...

Keyword(s):

Ion Channel ◽

Feasibility Study ◽

Neuronal Culture ◽

Odor Receptors

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Robust olfactory responses in the absence of odorant binding proteins

eLife ◽

10.7554/elife.51040 ◽

2019 ◽

Vol 8 ◽

Cited By ~ 17

Author(s):

Shuke Xiao ◽

Jennifer S Sun ◽

John R Carlson

Keyword(s):

Behavioral Response ◽

Binding Proteins ◽

Temporal Structure ◽

Olfactory Response ◽

Functional Type ◽

Odorant Binding Proteins ◽

Olfactory Responses ◽

Basiconic Sensilla ◽

Odorant Binding ◽

Odor Receptors

Odorant binding proteins (Obps) are expressed at extremely high levels in the antennae of insects, and have long been believed essential for carrying hydrophobic odorants to odor receptors. Previously we found that when one functional type of olfactory sensillum in Drosophila was depleted of its sole abundant Obp, it retained a robust olfactory response (Larter et al., 2016). Here we have deleted all the Obp genes that are abundantly expressed in the antennal basiconic sensilla. All of six tested sensillum types responded robustly to odors of widely diverse chemical or temporal structure. One mutant gave a greater physiological and behavioral response to an odorant that affects oviposition. Our results support a model in which many sensilla can respond to odorants in the absence of Obps, and many Obps are not essential for olfactory response, but that some Obps can modulate olfactory physiology and the behavior that it drives.

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A noseful of odor receptors

Trends in Neurosciences ◽

10.1016/0166-2236(91)90135-h ◽

1991 ◽

Vol 14 (7) ◽

pp. 270-272 ◽

Cited By ~ 55

Author(s):

Stuart Firestein

Keyword(s):

Odor Receptors

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The feasibility study of novel odor biosensor using dissociate neuronal culture expressing ion channel built-in odor receptors

Neuroscience Research ◽

10.1016/j.neures.2011.07.1340 ◽

2011 ◽

Vol 71 ◽

pp. e307

Author(s):

Norio Tanada ◽

Takeshi Sakurai ◽

Hidefumi Mitsuno ◽

Douglous Bakkum ◽

Ryohei Kanzaki ◽

...

Keyword(s):

Ion Channel ◽

Feasibility Study ◽

Neuronal Culture ◽

Odor Receptors

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Fishing for Genes: How the Largest Gene Family in the Mammalian Genome was Found (and Why Idiosyncrasy in Exploration Matters)

Perspectives on Science ◽

10.1162/posc_a_00375 ◽

2021 ◽

Vol 29 (4) ◽

pp. 359-387

Author(s):

Ann-Sophie Barwich

Keyword(s):

Gene Family ◽

Oral History ◽

Nobel Prize ◽

Multigene Family ◽

Experimental System ◽

Mammalian Genome ◽

Fundamental Question ◽

Individual Researcher ◽

The Individual ◽

Odor Receptors

Abstract In 1991, Linda Buck and Richard Axel identified the multigene family expressing odor receptors. Their discovery transformed research on olfaction overnight, and Buck and Axel were awarded the 2004 Nobel Prize in Physiology or Medicine. Behind this success lies another, less visible study about the methodological ingenuity of Buck. This hidden tale holds the key to answering a fundamental question in discovery analysis: What makes specific discovery tools fit their tasks? Why do some strategies turn out to be more fruitful than others? The fit of a method with an experimental system often establishes the success of a discovery. However, the underlying reasoning of discovery is hard to codify. These difficulties point toward an element of discovery analysis routinely sidelined as a mere biographical element in the philosophical analysis of science: the individual discoverer’s role. I argue that the individual researcher is not a replaceable epistemic element in discovery analysis. This article draws on contemporary oral history, including interviews with Buck and other actors key to developments in late 1980s olfaction.

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