QUANTITATIVE CHARACTERIZATION OF PROTEIN CONFORMATION IN SOLUTION BY VACUUM ULTRAVIOLET CIRCULAR DICHROISM

1981 ◽  
pp. 31-43 ◽  
Author(s):  
S. BRAHMS ◽  
J. BRAHMS
Keyword(s):  
Circular Dichroism ◽  
Protein Conformation ◽  
Vacuum Ultraviolet ◽  
Quantitative Characterization ◽  
Circular Dichroïsm

Dissociation and characterization of pilin isolated from Pseudomonas aeruginosa strains PAK and PAO

1982 ◽  
Vol 60 (9) ◽  
pp. 867-872 ◽  
Author(s):  
Tania H. Watts ◽  
Cyril M. Kay ◽  
William Paranchych
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Circular Dichroism ◽  
Protein Conformation ◽  
Axial Ratio ◽  
Sedimentation Equilibrium ◽  
Low Protein ◽  
Octyl Glucoside ◽  
Stokes Radius ◽  
Circular Dichroïsm

Pili isolated from Pseudomonas aeruginosa strains PAK and PAO have been dissociated into subunits using the detergent octyl glucoside. Circular dichroism studies indicated that no change in protein conformation occurs as a result of this treatment. Ultracentrifugation measurements showed that both pilins have a Stokes radius of 21 Å (1 Å = 0.1 nm) corresponding to an axial ratio of between 3:1 and 5:1, when approximated as prolate or oblate ellipsoids. Sedimentation equilibrium measurements show that even at low protein concentrations the pilin–detergent complex exists as a mixture of monomers and dimers.

scholarly journals Quantitative characterization of nanoscale polycrystalline magnets with electron magnetic circular dichroism

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Shunsuke Muto ◽  
Ján Rusz ◽  
Kazuyoshi Tatsumi ◽  
Roman Adam ◽  
Shigeo Arai ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Magnetic Circular Dichroism ◽  
Quantitative Characterization ◽  
Circular Dichroïsm

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

Conformation of membrane-bound proteins revealed by vacuum-ultraviolet circular-dichroism and linear-dichroism spectroscopy

2016 ◽  
Vol 84 (3) ◽  
pp. 349-359 ◽  
Author(s):  
Koichi Matsuo ◽  
Yasuyuki Maki ◽  
Hirofumi Namatame ◽  
Masaki Taniguchi ◽  
Kunihiko Gekko
Keyword(s):  
Circular Dichroism ◽  
Vacuum Ultraviolet ◽  
Linear Dichroism ◽  
Membrane Bound ◽  
Circular Dichroïsm
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