SkinDot: A modified full-skin transplantation technique

A neural crest transplantation technique is described for fish. As in other classes ofvertebrates, two pathways of neural crest migration can be distinguished: a lateroventral pathway between somites and ectoderm, and a medioventral pathway between somites and neural tube/notochord. In this paper evidence is presented for a neural crest origin of spinal ganglion cells and pigment cells, and indication for such an origin is obtained for sympathetic and enteric ganglion cells and for cells that are probably homologues to adrenomedullary and paraganglion cells in the future kidney area. The destiny of neural crest cells near the developing lateral-line sense organs is discussed. When grafted into the yolk, neural crest cells or neural tube cells appear to differentiate into ‘periblast cells’; this suggests a highly activating influence of the yolk. Many neural crest cells are found around the urinary ducts and, when grafted below the notochord, even within the urinary duct epithelium. These neural crest cells do not invade the gut epithelium, even when grafted adjacent to the developing gut. Consequently enteroendocrine cells in fish are not likely to have a trunkor rhombencephalic neural crest origin. Another possible origin of these cells will be proposed.

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Meniscal allograft transplantation in rabbit

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201520140417 ◽

2015 ◽

Vol 87 (4) ◽

pp. 2205-2216

Author(s):

LIANA M. VILELA ◽

RICARDO J. DEL CARLO ◽

EDSON V. Melo FILHO ◽

LUKIYA S.C. FAVARATO ◽

TATIANA S. DUARTE ◽

...

Keyword(s):

Collagen Type I ◽

Collagen Fibers ◽

The Body ◽

Type I ◽

Meniscal Allograft Transplantation ◽

Allograft Transplantation ◽

Meniscal Allograft ◽

Preservation Method ◽

Transplantation Technique ◽

Partial Fixation

This study evaluated the technique for meniscal allograft transplantation using allografts preserved in glycerin 98% in rabbits. Euthanasia was performed at 70 days to compare the transplanted (TM1 to TM16) versus the contralateral meniscus (OM1 to OM16). Sixteen menisci, 8 transplanted and 8 contralateral, were submitted to gross examination, histomorphometric analysis for identification and quantification of cellular type, and for quantification and distribution of collagen fibers. A revascularization study was conducted in all of the other samples. Lengths of the OM varied from 0.9 to 1.0 cm and two TM were smaller. All TM were completely attached to the synovial membrane, except for one case that presented partial fixation. Both, TM and OM had similar amounts of chondrocytes, fibroblasts and fibrocytes, and at the horns, chondrocytes were predominant. The collagen fibers in TM were well organized throughout the body, and disorganized at the horns. These fibers in OM were organized. The amounts of collagen type I and III, and the vascularization of the perimeniscal tissue and of the edge were similar in OM and TM. These results demonstrated graft integration and thus this transplantation technique and preservation method may be recommended.

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Posterior Segment Approach for Subretinal Transplantation or Injection in the Canine Model

Cell Transplantation ◽

10.3727/000000001783986710 ◽

2001 ◽

Vol 10 (3) ◽

pp. 317-327 ◽

Cited By ~ 6

Author(s):

Maria E. Verdugo ◽

Julie Alling ◽

Eliot S. Lazar ◽

Manuel Del Cerro ◽

Jharna Ray ◽

...

Keyword(s):

Light Microscopy ◽

Canine Model ◽

Posterior Segment ◽

Fundus Photography ◽

Subretinal Space ◽

Rpe Cells ◽

Injection Volume ◽

Clinical Complications ◽

Optimal Injection ◽

Transplantation Technique

A posterior segment approach for cell transplantation or injection into the subretinal space of the dog has been developed. Controlled penetration to the subretinal space was achieved using a 29-gauge injection cannula, either blunted or with a 30° sharpened bevel, and partially ensheathed with moveable plastic tubing. Depending on the injection volume used, the retina detached, and the fluid was reabsorbed within 1 – 3 weeks, although for smaller volumes the retina reattached within a matter of days. The optimal injection volume used was between 100 and 150 μl, or two injections of 55 μl each. By ophthalmoscopy following the surgery, it was possible to serially monitor the injection site and retinal bleb through fundus photography. Light microscopy demonstrates the distribution of stable, viable RPE cells in the subretinal space up to 6 months. The transplantation technique developed for the dog is atraumatic and free from any major surgical or clinical complications. It can be readily used to deliver cells or fluids to localized regions of the subretinal space.

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Murine Full-thickness Skin Transplantation

Journal of Visualized Experiments ◽

10.3791/55105 ◽

2017 ◽

Cited By ~ 7

Author(s):

Chih–Hsien Cheng ◽

Chen–Fang Lee ◽

Madeline Fryer ◽

Georg J. Furtmüller ◽

Byoungchol Oh ◽

...

Keyword(s):

Skin Transplantation ◽

Thickness Skin

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Autogenous Tooth Transplantation: A Clinical and Histological Investigation

British Journal of Orthodontics ◽

10.1179/bjo.1.2.27 ◽

1974 ◽

Vol 1 (2) ◽

pp. 27-33 ◽

Cited By ~ 17

Author(s):

B. B. J. Lovius ◽

J. D. Atherton ◽

T. H. M. Wynne ◽

L. D. Finch

Keyword(s):

Surgical Procedure ◽

Histological Investigation ◽

Tooth Transplantation ◽

Transplantation Technique ◽

Time Of Operation

The most successful autogenous tooth transplantation technique of which we have had experience in Liverpool is that described by Thonner (1969). Our results have been sufficiently encouraging to regard the technique as a normal orthodontic/surgical procedure and to be able to tell the patient that the chances of success are good. The technique consists of the removal of the tooth with the minimum of trauma and while the new socket is being prepared the tooth is kept in the patient's serum at 37°C. The tooth is not root filled at the time of operation.

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