Whole-mount in situ detection of microRNAs on Arabidopsis tissues using Zip Nucleic Acid probes

2013 ◽  
Vol 434 (1) ◽  
pp. 60-66 ◽  
Author(s):  
M. Begheldo ◽  
F.A. Ditengou ◽  
G. Cimoli ◽  
S. Trevisan ◽  
S. Quaggiotti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Whole Mount ◽  
In Situ Detection ◽  
Zip Nucleic Acid

Corrigendum to “Whole-mount in situ detection of microRNAs on Arabidopsis tissues using Zip Nucleic Acid probes” [Anal. Biochem. 434 (2013) 60–66]

2013 ◽  
Vol 437 (1) ◽  
pp. 39
Author(s):  
M. Begheldo ◽  
F.A. Ditengou ◽  
G. Cimoli ◽  
S. Trevisan ◽  
S. Quaggiotti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Whole Mount ◽  
In Situ Detection ◽  
Zip Nucleic Acid

scholarly journals Procedures for in situ monitoring of gene methylation in cancer prediction (Literature Review)

Akademos ◽  
2021 ◽  
pp. 89-95
Author(s):  
Victor Popescu ◽  
◽  
Mariana Jian ◽  
Tatiana Malcova ◽  
◽  
...  
Keyword(s):  
Literature Review ◽  
In Situ Monitoring ◽  
Gene Methylation ◽  
Cancer Prediction ◽  
Human Genes ◽  
Whole Mount ◽  
In Situ Detection

The purpose of this article is to synthesize data from the specialty literature on the best performing procedures for the in situ detection and monitoring of the phenomenon of gene methylation, to analyze the protocol for the realization of the whole mount MeFISH technology and to review the human genes that are characterized by methylation in cancer.

scholarly journals In Situ detection of apoptosis during embryogenesis with Annexin V: From whole mount to ultrastructure

Cytometry ◽  
1997 ◽  
Vol 29 (4) ◽  
pp. 313-320 ◽  
Author(s):  
Stefan M. van den Eijnde ◽  
Antonius J.M. Luijsterburg ◽  
Lenard Boshart ◽  
Chris I. De Zeeuw ◽  
Jan Hein van Dierendonck ◽  
...  
Keyword(s):  
Annexin V ◽  
Whole Mount ◽  
In Situ Detection

scholarly journals Nonspecific binding of nucleic acid probes to Paneth cells in the gastrointestinal tract with in situ hybridization.

1992 ◽  
Vol 40 (10) ◽  
pp. 1613-1618 ◽  
Author(s):  
K L Garrett ◽  
M D Grounds ◽  
M W Beilharz
Keyword(s):  
In Situ Hybridization ◽  
Gastrointestinal Tract ◽  
Nucleic Acid ◽  
Paneth Cells ◽  
Proteinase K ◽  
Hybridization Technique ◽  
Nonspecific Binding ◽  
Nucleic Acid Probes ◽  
Hybridization Data

Nonspecific binding of a number of unrelated nucleic acid probes to cells in the crypts of Lieberkuhn was observed in the small intestine of mice with the in situ hybridization technique. Hybridization signal was localized to cells which, by virtue of their histological position, represented Paneth cells. This signal could not be removed by RNAse, DNAse, or proteinase K treatment, and was not removed after high-stringency washing conditions. This report indicates that caution must be exercised in the interpretation of in situ hybridization data when looking for nucleic acid sequences in the gastrointestinal tract.

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