Comparison of Effective Organisms in Bioremediation Processes: Potential Use of Nucleic Acid Probes to Estimate Cyanide Degradation in Situ

2003 ◽  
pp. 21-28
Author(s):  
M. Barclay ◽  
A. Tabouret ◽  
J. C. Day ◽  
I. P. Thompson ◽  
C. J. Knowles ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Cyanide Degradation ◽  
Potential Use ◽  
Bioremediation Processes

scholarly journals Nonspecific binding of nucleic acid probes to Paneth cells in the gastrointestinal tract with in situ hybridization.

1992 ◽  
Vol 40 (10) ◽  
pp. 1613-1618 ◽  
Author(s):  
K L Garrett ◽  
M D Grounds ◽  
M W Beilharz
Keyword(s):  
In Situ Hybridization ◽  
Gastrointestinal Tract ◽  
Nucleic Acid ◽  
Paneth Cells ◽  
Proteinase K ◽  
Hybridization Technique ◽  
Nonspecific Binding ◽  
Nucleic Acid Probes ◽  
Hybridization Data

Nonspecific binding of a number of unrelated nucleic acid probes to cells in the crypts of Lieberkuhn was observed in the small intestine of mice with the in situ hybridization technique. Hybridization signal was localized to cells which, by virtue of their histological position, represented Paneth cells. This signal could not be removed by RNAse, DNAse, or proteinase K treatment, and was not removed after high-stringency washing conditions. This report indicates that caution must be exercised in the interpretation of in situ hybridization data when looking for nucleic acid sequences in the gastrointestinal tract.

Corrigendum to “Whole-mount in situ detection of microRNAs on Arabidopsis tissues using Zip Nucleic Acid probes” [Anal. Biochem. 434 (2013) 60–66]

2013 ◽  
Vol 437 (1) ◽  
pp. 39
Author(s):  
M. Begheldo ◽  
F.A. Ditengou ◽  
G. Cimoli ◽  
S. Trevisan ◽  
S. Quaggiotti ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Whole Mount ◽  
In Situ Detection ◽  
Zip Nucleic Acid
Sign in / Sign up

Export Citation Format

Share Document