Biocompatible Electrochemical Sensor Based on Platinum-Nickel Alloy Nanoparticles for In Situ Monitoring of Hydrogen Sulfide in Breast Cancer Cells

Hydrogen sulfide (H2S), an endogenous gasotransmitter, is produced in mammalian systems and is closely associated with pathological and physiological functions. Nevertheless, the complete conversion of H2S is still unpredictable owing to the limited number of sensors for accurate and quantitative detection of H2S in biological samples. In this study, we constructed a disposable electrochemical sensor based on PtNi alloy nanoparticles (PtNi NPs) for sensitive and specific in situ monitoring of H2S released by human breast cancer cells. PtNi alloy NPs with an average size of 5.6 nm were prepared by a simple hydrothermal approach. The conversion of different forms of sulfides (e.g., H2S, HS−, and S2−) under various physiological conditions hindered the direct detection of H2S in live cells. PtNi NPs catalyze the electrochemical oxidation of H2S in a neutral phosphate buffer (PB, pH 7.0). The PtNi-based sensing platform demonstrated a linear detection range of 0.013–1031 µM and the limit of detection was 0.004 µM (S/N = 3). Moreover, the PtNi sensor exhibited a sensitivity of 0.323 μA μM−1 cm−2. In addition, the stability, repeatability, reproducibility, and anti-interference ability of the PtNi sensor exhibited satisfactory results. The PtNi sensor was able to successfully quantify H2S in pond water, urine, and saliva samples. Finally, the biocompatible PtNi electrode was effectively employed for the real-time quantification of H2S released from breast cancer cells and mouse fibroblasts.

Long-term effects of a chemical decontamination procedure on the corrosion state of the heat exchanger tubes of steam generators

Our previous studies have revealed that a ”hybrid” structure of the amorphous and crystalline phases is formed in the outermost surface region of the austenitic stainless steel tubes of steam generators (SGs) as an undesired consequence of the industrial application of the AP-CITROX (AP: alkaline permanganate; CITROX: citric and oxalic acid) decontamination technology. The formation of this mobile oxide-layer increased the amount of the corrosion products in the primary circuit significantly, resulting in magnetite deposition on fuel assemblies. Owing to the fact that there is no investigation method available for the in-situ monitoring of the inner surfaces of heat exchanger tubes, a research project based on sampling as well as on ex-situ electrochemical and surface analytical measurements was elaborated. Within the frame of this project, comprehensive investigation of the general corrosion state and metallographic features of 36 stainless steel specimens, cut out from various locations of the 21 steam generators of the Paks NPP in the time period of 2000-2007 has been performed. The present work gives a brief overview on the general corrosion state of the heat exchanger tubes of SGs, concerning the long-term effects of the AP-CITROX procedure on the chemical composition and structure of the protective oxide-layer.

A FRET Approach to Detect Paraoxon among Organophosphate Pesticides Using a Fluorescent Biosensor

The development of faster, sensitive and real-time methods for detecting organophosphate (OP) pesticides is of utmost priority in the in situ monitoring of these widespread compounds. Research on enzyme-based biosensors is increasing, and a promising candidate as a bioreceptor is the thermostable enzyme esterase-2 from Alicyclobacillus acidocaldarius (EST2), with a lipase-like Ser–His–Asp catalytic triad with a high affinity for OPs. This study aimed to evaluate the applicability of Förster resonance energy transfer (FRET) as a sensitive and reliable method to quantify OPs at environmentally relevant concentrations. For this purpose, the previously developed IAEDANS-labelled EST2-S35C mutant was used, in which tryptophan and IAEDANS fluorophores are the donor and the acceptor, respectively. Fluorometric measurements showed linearity with increased EST2-S35C concentrations. No significant interference was observed in the FRET measurements due to changes in the pH of the medium or the addition of other organic components (glucose, ascorbic acid or yeast extract). Fluorescence quenching due to the presence of paraoxon was observed at concentrations as low as 2 nM, which are considered harmful for the ecosystem. These results pave the way for further experiments encompassing more complex matrices.

In situ monitoring of the electrical property of carbon nanotube thin film in floating catalyst chemical vapor deposition

In floating catalyst chemical vapor deposition (FCCVD), when a carbon nanotube (CNT) network film is produced by filter collection, the film thickness is adjusted by controlling the collection time. However, even with consistent synthesis parameters, the synthesis condition in FCCVD changes constantly depending on the carbon and catalyst adhesion to the inner wall of the reaction tube. Thus, the rate of synthesis changes, making it difficult to obtain the target film thickness repeatedly and stably. We propose a method of monitoring CNT film thickness and percolation threshold by the in situ measurement of the electrical impedance during the deposition. The time evolution of the measured impedance is reproducible by an equivalent electrical circuit simulation.

In Situ Monitoring of Corrosion under Insulation Using Electrochemical and Mass Loss Measurements

Corrosion under insulation (CUI) refers to the external corrosion of piping and vessels when they are encapsulated in thermal insulation. To date, very limited information (especially electrochemical data) is available for these “difficult-to-test” CUI conditions. This study was aimed at developing a novel electrochemical sensing method for in situ CUI monitoring and analysis. Pt-coated Ti wires were used to assemble a three-electrode electrochemical cell over a pipe surface covered by thermal insulation. The CUI behavior of X70 carbon steel (CS) and 304 stainless steel (SS) under various operating conditions was investigated using mass loss, linear polarization resistance (LPR), and electrochemical impedance spectroscopy (EIS) measurements. It was found that both the consecutive wet and dry cycles and cyclic temperatures accelerated the progression of CUI. LPR and EIS measurements revealed that the accelerated CUI by thermal cycling was due to the reduced polarization resistance and deteriorated corrosion film. Enhanced pitting corrosion was observed on all tested samples after thermal cycling conditions, especially for CS samples. The proposed electrochemical technique demonstrated the ability to obtain comparable corrosion rates to conventional mass loss data. In addition to its potential for in situ CUI monitoring, this design could be further applied to rank alloys, coatings, and inhibitors under more complex exposure conditions.

Microstructure Refinement on Crevice Corrosion of High-Speed Rail Steel U75V Visualized by an In Situ Monitoring System

Rail foot covered by a fastener will suffer from crevice corrosion, leading to thinning and localized attack of crevice interior posing a risk of failure. This work investigated crevice corrosion behavior of a typical pearlitic high-speed rail steel U75V, focusing for the first time on the effect of pearlitic microstructure refinement achieved by heat treatment with different cooling rates 2, 5, and 10°C/s. Under anodic polarization, localized dissolved spots presented on the as-received sample, where crevice corrosion mostly initiated from. For cooling rates 2 and 5°C/s, localized dissolved spots were also observed but crevice corrosion was mostly presented as general corrosion instead of from local spots, ascribed to enhanced tendency of uniform dissolution due to microstructure refinement and homogenization. For cooling rate 10°C/s, crevice corrosion expanded flocculently, ascribed to preferential dissolution of pearlitic nodules with entangled cementite due to over refinement. Crevice corrosion was obviously accelerated by microstructure refinement. Cooling rates 5 and 10°C/s led to the fastest and slowest expansion of the corroded area, respectively, while the corrosion depth was just the opposite based on the same amount of metal loss. This work provides important information regarding the effect of pearlitic microstructure refinement on crevice corrosion and introduces a facile method for in situ monitoring of crevice corrosion.

Monitoring Dynamic Recrystallisation in Bioresorbable Alloy Mg-1Zn-0.2Ca by Means of an In Situ Acoustic Emission Technique

The tensile behaviour of the biocompatible alloy Mg-1Zn-0.2Ca (in wt.%) in the fine-grained state, obtained by severe plastic deformation via multiaxial isothermal forging, has been investigated in a wide range of temperatures (20 ÷ 300) °C and strain rates (5 × 10−4 ÷ 2 × 10−2) s−1 with the measurements of acoustic emission (AE). The dependences of mechanical properties, including the yield stress, ultimate strength, ductility, and the strain-hardening rate, on the test temperature and strain rate, were obtained and discussed. It is shown for the first time that an acoustic emission method is an effective tool for in situ monitoring of the dynamic recrystallisation (DRX) process. The specific behaviour of the acoustic emission spectral density reflected by its median frequency as a function of strain at various temperatures can serve as an indicator of the DRX process’s completeness.