GC-FID-based quantification of the sum of the three forms of vitamin B3 from animal liver

2020 ◽  
Vol 601 ◽  
pp. 113778
Author(s):  
Michael Hämmerle ◽  
Minh Hien Le ◽  
Omid Hekmat
Keyword(s):  
Vitamin B3 ◽  
Animal Liver

scholarly journals Equilibrative Nucleoside Transporters Mediate the Import of Nicotinamide Riboside and Nicotinic Acid Riboside into Human Cells

2021 ◽  
Vol 22 (3) ◽  
pp. 1391
Author(s):  
Andrey Kropotov ◽  
Veronika Kulikova ◽  
Kirill Nerinovski ◽  
Alexander Yakimov ◽  
Maria Svetlova ◽  
...  
Keyword(s):  
Nicotinic Acid ◽  
Mammalian Cells ◽  
Experimental Models ◽  
The Body ◽  
Nucleoside Transporters ◽  
Hek293 Cells ◽  
Nucleoside Transporter ◽  
Vitamin B3 ◽  
Animal Cells ◽  
Nicotinamide Riboside

Nicotinamide riboside (NR), a new form of vitamin B3, is an effective precursor of nicotinamide adenine dinucleotide (NAD+) in human and animal cells. The introduction of NR into the body effectively increases the level of intracellular NAD+ and thereby restores physiological functions that are weakened or lost in experimental models of aging and various pathologies. Despite the active use of NR in applied biomedicine, the mechanism of its transport into mammalian cells is currently not understood. In this study, we used overexpression of proteins in HEK293 cells, and metabolite detection by NMR, to show that extracellular NR can be imported into cells by members of the equilibrative nucleoside transporter (ENT) family ENT1, ENT2, and ENT4. After being imported into cells, NR is readily metabolized resulting in Nam generation. Moreover, the same ENT-dependent mechanism can be used to import the deamidated form of NR, nicotinic acid riboside (NAR). However, NAR uptake into HEK293 cells required the stimulation of its active utilization in the cytosol such as phosphorylation by NR kinase. On the other hand, we did not detect any NR uptake mediated by the concentrative nucleoside transporters (CNT) CNT1, CNT2, or CNT3, while overexpression of CNT3, but not CNT1 or CNT2, moderately stimulated NAR utilization by HEK293 cells.

scholarly journals Determination of Vitamin B3 Vitamer (Nicotinamide) and Vitamin B6 Vitamers in Human Hair Using LC-MS/MS

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4487
Author(s):  
Sundus M. Sallabi ◽  
Aishah Alhmoudi ◽  
Manal Alshekaili ◽  
Iltaf Shah
Keyword(s):  
Human Hair ◽  
Vitamin B6 ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Reversed Phase ◽  
Water Soluble ◽  
Vitamin B3 ◽  
Hair Samples ◽  
Pyridoxal Hydrochloride ◽  
Vitamin B3 Deficiency

Water-soluble B vitamins participate in numerous crucial metabolic reactions and are critical for maintaining our health. Vitamin B deficiencies cause many different types of diseases, such as dementia, anaemia, cardiovascular disease, neural tube defects, Crohn’s disease, celiac disease, and HIV. Vitamin B3 deficiency is linked to pellagra and cancer, while niacin (or nicotinic acid) lowers low-density lipoprotein (LDL) and triglycerides in the blood and increases high-density lipoprotein (HDL). A highly sensitive and robust liquid chromatography–tandem mass spectroscopy (LC/MS-MS) method was developed to detect and quantify a vitamin B3 vitamer (nicotinamide) and vitamin B6 vitamers (pyridoxial 5′-phosphate (PLP), pyridoxal hydrochloride (PL), pyridoxamine dihydrochloride (PM), pridoxamine-5′-phosphate (PMP), and pyridoxine hydrochloride (PN)) in human hair samples of the UAE population. Forty students’ volunteers took part in the study and donated their hair samples. The analytes were extracted and then separated using a reversed-phase Poroshell EC-C18 column, eluted using two mobile phases, and quantified using LC/MS-MS system. The method was validated in human hair using parameters such as linearity, intra- and inter-day accuracy, and precision and recovery. The method was then used to detect vitamin B3 and B6 vitamers in the human hair samples. Of all the vitamin B3 and B6 vitamers tested, only nicotinamide was detected and quantified in human hair. Of the 40 samples analysed, 12 were in the range 100–200 pg/mg, 15 in the range 200–500 pg/mg, 9 in the range of 500–4000 pg/mg. The LC/MS-MS method is effective, sensitive, and robust for the detection of vitamin B3 and its vitamer nicotinamide in human hair samples. This developed hair test can be used in clinical examination to complement blood and urine tests for the long-term deficiency, detection, and quantification of nicotinamide.

Antifibrotic activity of galangin, a novel function evaluated in animal liver fibrosis model

2013 ◽  
Vol 36 (2) ◽  
pp. 288-295 ◽  
Author(s):  
Xinhui Wang ◽  
Guoqing Gong ◽  
Wenhui Yang ◽  
Yunzhan Li ◽  
Meiling Jiang ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Animal Liver

Investigations on acyl group carriers. XIII. A new method of synthesis of D-(+)-S-sulfopantetheine and its conversion into vitamin B3 coenzyme forms

1978 ◽  
Vol 12 (8) ◽  
pp. 1028-1031
Author(s):  
V. M. Kopelevich ◽  
A. V. Lysenkova ◽  
A. G. Moiseenok ◽  
V. I. Gunar
Keyword(s):  
New Method ◽  
Vitamin B3
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