Development of a sensitive sandwich-ELISA assay for reliable detection of fish residues in foods

Purpose: Neuregulins (NRG) are growth factors that bind to receptors of the erbB family, and are known to mediate a number of processes involved in diverse tissues. Neuregulin-1β is expressed in skeletal muscle and is activated by exercise. We hypothesized that NRG-1β might circulate in the bloodstream and increase as a consequence of physical activity. A study was conducted in healthy subjects to determine if NRG-1β is immunodetectable in human serum, and if so whether levels relate acutely or chronically to exercise. Methods: Nine healthy men underwent three bouts of exercise of varying degrees of intensity on a bicycle ergometer over a period of three weeks. Cardio-respiratory fitness was determined by measurement of maximal oxygen uptake (VO2max). Serum was sampled prior to and immediately after each session (up to 30 minutes post) and serum NRG-1ß was quantified utilizing an indirect sandwich ELISA assay developed in our lab. Results: Across subjects, mean serum NRG-1β levels ranged from 32 ng/mL to 473 ng/mL. Individual subjects showed relatively stable levels during the study period that did not change acutely after exercise. Serum NRG-1β demonstrated a positive correlation with VO2max (r2=0.49, p =.044). Conclusions: These preliminary observations suggest that at least in healthy men, serum NRG-1β is an indicator of cardio- respiratory fitness and does not change acutely with exercise.

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72 A new highly sensitive rapid sandwich ELISA assay for the determination of blood coagulation factor XIII in human plasma

Fibrinolysis and Proteolysis ◽

10.1016/s0268-9499(98)80101-x ◽

1998 ◽

Vol 12 ◽

pp. 26

Keyword(s):

Human Plasma ◽

Blood Coagulation ◽

Factor Xiii ◽

Sandwich Elisa ◽

Coagulation Factor ◽

Elisa Assay ◽

Coagulation Factor Xiii ◽

Highly Sensitive ◽

Sandwich Elisa Assay

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Monoclonal Antibodies to Sea Cucumber Polysaccharide and Their Use in a Sandwich ELISA Assay

Hybridoma ◽

10.1089/hyb.2011.0025 ◽

2011 ◽

Vol 30 (4) ◽

pp. 381-385 ◽

Cited By ~ 2

Author(s):

Junjun Wang ◽

Hu Zhao ◽

Ying Zheng ◽

Wei Liu ◽

Hua Zhou ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Sea Cucumber ◽

Sandwich Elisa ◽

Elisa Assay ◽

Sandwich Elisa Assay

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Sandwich ELISA Assay for the Quantitation of Palytoxin and Its Analogs in Natural Samples

Environmental Science & Technology ◽

10.1021/es304222t ◽

2013 ◽

Vol 47 (4) ◽

pp. 2034-2042 ◽

Cited By ~ 20

Author(s):

S. Boscolo ◽

M. Pelin ◽

M. De Bortoli ◽

G. Fontanive ◽

A. Barreras ◽

...

Keyword(s):

Sandwich Elisa ◽

Elisa Assay ◽

Sandwich Elisa Assay

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A Simple, Quick One-step ELISA Assay for the Determination of Complex Plasma Factor XIII (A2B2)

Thrombosis and Haemostasis ◽

10.1055/s-0037-1613798 ◽

2000 ◽

Vol 83 (02) ◽

pp. 268-273 ◽

Cited By ~ 60

Author(s):

Éva Katona ◽

Gizella Haramura ◽

Levente Kárpáti ◽

József Fachet ◽

László Muszbek

Keyword(s):

Factor Xiii ◽

Sandwich Elisa ◽

High Sensitivity ◽

Reference Interval ◽

Elisa Assay ◽

Plasma Factor ◽

Sandwich Elisa Assay ◽

One Step ◽

A Subunit

SummaryA new highly sensitive sandwich ELISA assay was developed for the determination of plasma factor XIII (FXIII). Plasma FXIII is a tetrameric complex of two types of subunits (A2B2). A biotinylated monoclonal capture-antibody against the B-subunit and a peroxidase-labelled monoclonal tag-antibody against the A-subunit were added to the plasma dilution and the amount of the complex attached to streptavidincoated microplate was quantitated by measuring peroxidase activity. Only the tetrameric plasma FXIII reacted in the assay, non-complexed A or B subunits showed no reaction. The assay is linear up-to 40 µg/L of FXIII in the assay mixture. It is a quick one-step assay which can be performed within two hours. At normal and low FXIII concentration within batch reproducibility was 2.0% and 3.3%, day to day variation was 5.5% and 8.7%, respectively. Its high sensitivity allows reliable measurement at FXIII concentrations below 1% of normal average. Plasma samples can be stored for the assay at –20° C for at least one month. Plasma levels of healthy individuals were normally distributed and no gender difference was observed. A reference interval of 14-28 mg/L (67-133%) was established.

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Development of a sandwich ELISA assay for measuring bovine soluble type II IL-1 receptor (IL1R2) concentration in serum and milk

Cytokine ◽

10.1016/j.cyto.2005.08.007 ◽

2005 ◽

Vol 32 (3-4) ◽

pp. 132-136 ◽

Cited By ~ 7

Author(s):

Katsuro Hagiwara ◽

Keiko Kitajima ◽

Hitoki Yamanaka ◽

Rikio Kirisawa ◽

Hiroshi Iwai

Keyword(s):

Sandwich Elisa ◽

Elisa Assay ◽

Type Ii ◽

Sandwich Elisa Assay

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Development of a Double Antibody Sandwich Elisa Assay for the Diagnosis of Angiostrongyliasis

Journal of Parasitology ◽

10.1645/ge-2590.1 ◽

2011 ◽

Vol 97 (4) ◽

pp. 721-724 ◽

Cited By ~ 5

Author(s):

Mu-Xin Chen ◽

Kun Wang ◽

Lin Ai ◽

Wen-Hao Yan ◽

Liang Peng ◽

...

Keyword(s):

Sandwich Elisa ◽

Elisa Assay ◽

Double Antibody ◽

Sandwich Elisa Assay

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Quantitative Detection of Pork Contamination in Cooked Meat Products by ELISA

Journal of AOAC International ◽

10.5740/jaoacint.17-0036 ◽

2018 ◽

Vol 101 (3) ◽

pp. 810-816 ◽

Cited By ~ 5

Author(s):

Cortlandt P Thienes ◽

Jongkit Masiri ◽

Lora A Benoit ◽

Brianda Barrios-Lopez ◽

Santosh A Samuel ◽

...

Keyword(s):

Validation Study ◽

Sandwich Elisa ◽

Meat Products ◽

Quantitative Detection ◽

Elisa Assay ◽

Analytical Sensitivity ◽

Sandwich Elisa Assay ◽

Analytical Range ◽

Cooked Meat ◽

Cooked Meat Products

Abstract Recent news of many cases of adulteration of meats with pork has bolstered the need for a way to detect and quantify the unwanted contamination of pork in other meats. To address this need, Microbiologique, Inc. has produced a sandwich ELISA assay that can rapidly quantify the presence of pork in cooked horse, beef, chicken, goat, and lamb meats. We carried out a validation study and showed that this assay has an analytical sensitivity of 0.00014 and 0.00040% (w/v) for cooked and autoclaved pork, respectively, and an analytical range of quantitation of 0.05–3.2% (w/v) in the absence of other meats. The assay can measure pork contamination down to 0.1% (w/w) in the presence of cooked horse, beef, chicken, goat, and lamb meats. The assay is quick and can be completed in 1 h and 10 min.

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