A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B1 in food samples

2006 ◽  
Vol 580 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ying Quan ◽  
Yan Zhang ◽  
Shuo Wang ◽  
Nanju Lee ◽  
Ivan R. Kennedy
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

Optimization and validation of enzyme-linked immunosorbent assay for the determination of endosulfan residues in food samples

2008 ◽  
Vol 43 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Yan Zhang ◽  
Jun W. Liu ◽  
Wen J. Zheng ◽  
Lei Wang ◽  
Hong Y. Zhang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

Rapid Determination of Fumonisin B1in Food Samples by Enzyme-Linked Immunosorbent Assay and Colloidal Gold Immunoassay

2006 ◽  
Vol 54 (7) ◽  
pp. 2491-2495 ◽  
Author(s):  
Shuo Wang ◽  
Ying Quan ◽  
Nanju Lee ◽  
Ivan R. Kennedy
Keyword(s):  
Immunosorbent Assay ◽  
Colloidal Gold ◽  
Rapid Determination ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Estimation of aflatoxins in peanut or maize by enzyme linked immunosorbent assay

2016 ◽  
Vol 11 (3) ◽  
pp. 628 ◽  
Author(s):  
Shruti Shukla
Keyword(s):  
Sample Preparation ◽  
Immunosorbent Assay ◽  
Video Clip ◽  
Test Sample ◽  
Microtiter Plate ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Assay Procedure ◽  
Exact Amount

<p>Aflatoxin are potentially toxic metabolites produced by microscopic fungi when grow on food. Hence, determining content of mycotoxins such as aflatoxin has significant pharmaceutical value. This study reports step-by-step visual demonstration of determination of aflatoxin contents in peanut and maize (corn)-based food samples through employing enzyme linked immunosorbent assay (ELISA). The method is based on direct competitive ELISA in a micro-well format which allows determination of exact amount of aflatoxin in food samples as parts per billion (ppb), and the results are calculated using Neogen’s Veratox data reduction software that converts the absorbance values into quantities. This visual experiment will certainly contribute knowledge regarding importance and applicability of the ELISA method for determining aflatoxin content in variety of food samples avoided of analytical issues such as sampling, laborious sample preparation, and critical selection of analytical methods.</p><p><strong>Video Clip</strong></p><p><a href="https://www.youtube.com/v/iWR1gt_u2jo">Test sample preparation:</a> 7 min 12 sec<br /><a href="https://www.youtube.com/v/VY_iwjpXFZI">Assay procedure:</a> 5 min 23 sec<br /><a href="https://www.youtube.com/v/mcdSp9ZRE7Q">Measurement of absorbance by Tecan ELISA microtiter plate reader:</a> 2 min 57 sec</p><p> </p>

Determination of hydrolysed fumonisin B1(HFB1) in corn by competitive direct enzyme‐linked immunosorbent assay

1996 ◽  
Vol 13 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Chris M. Maragos ◽  
Ronald D. Plattner ◽  
Steven D. Miklasz
Keyword(s):  
Immunosorbent Assay ◽  
Fumonisin B1 ◽  
Enzyme Linked Immunosorbent Assay

Biotin-avidin Mediated Competitive Enzyme-linked Immunosorbent Assay for Determination of Ketamine

2010 ◽  
Vol 38 (1) ◽  
pp. 117-120
Author(s):  
Wen-Di ZHANG ◽  
Ping SU ◽  
Yi YANG ◽  
Zhen-Quan GUO
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

scholarly journals Development and optimization of an indirect enzyme-linked immunosorbent assay for the determination of Hexoestrol

2006 ◽  
Vol 17 (3-4) ◽  
pp. 157-171 ◽  
Author(s):  
Liying Wang ◽  
Chuanlai Xu ◽  
Chifang Peng
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

Determination of plasma pantothenic acid by indirect enzyme linked immunosorbent assay

1990 ◽  
Vol 10 (4) ◽  
pp. 439-448 ◽  
Author(s):  
Won O. Song ◽  
Allen Smith ◽  
Carl Wittwer ◽  
Bonita Wyse ◽  
Gaurth Hansen
Keyword(s):  
Immunosorbent Assay ◽  
Pantothenic Acid ◽  
Enzyme Linked Immunosorbent Assay

Determination of the Folate Content in Cladodes of Nopal (Opuntia ficus indica) by Microbiological Assay UtilizingLactobacillus casei(ATCC 7469) and Enzyme-Linked Immunosorbent Assay

2010 ◽  
Vol 58 (10) ◽  
pp. 6472-6475 ◽  
Author(s):  
Tania Breshkovskaya Ortiz-Escobar ◽  
Maria Elena Valverde-González ◽  
Octavio Paredes-López
Keyword(s):  
Immunosorbent Assay ◽  
Microbiological Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Opuntia Ficus Indica
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