scholarly journals Estimation of aflatoxins in peanut or maize by enzyme linked immunosorbent assay

2016 ◽  
Vol 11 (3) ◽  
pp. 628 ◽  
Author(s):  
Shruti Shukla
Keyword(s):  
Sample Preparation ◽  
Immunosorbent Assay ◽  
Video Clip ◽  
Test Sample ◽  
Microtiter Plate ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Assay Procedure ◽  
Exact Amount

<p>Aflatoxin are potentially toxic metabolites produced by microscopic fungi when grow on food. Hence, determining content of mycotoxins such as aflatoxin has significant pharmaceutical value. This study reports step-by-step visual demonstration of determination of aflatoxin contents in peanut and maize (corn)-based food samples through employing enzyme linked immunosorbent assay (ELISA). The method is based on direct competitive ELISA in a micro-well format which allows determination of exact amount of aflatoxin in food samples as parts per billion (ppb), and the results are calculated using Neogen’s Veratox data reduction software that converts the absorbance values into quantities. This visual experiment will certainly contribute knowledge regarding importance and applicability of the ELISA method for determining aflatoxin content in variety of food samples avoided of analytical issues such as sampling, laborious sample preparation, and critical selection of analytical methods.</p><p><strong>Video Clip</strong></p><p><a href="https://www.youtube.com/v/iWR1gt_u2jo">Test sample preparation:</a> 7 min 12 sec<br /><a href="https://www.youtube.com/v/VY_iwjpXFZI">Assay procedure:</a> 5 min 23 sec<br /><a href="https://www.youtube.com/v/mcdSp9ZRE7Q">Measurement of absorbance by Tecan ELISA microtiter plate reader:</a> 2 min 57 sec</p><p> </p>

Determination of soya proteins in food using an enzyme-linked immunosorbent assay procedure

1981 ◽  
Vol 32 (2) ◽  
pp. 157-165 ◽  
Author(s):  
Christopher H. S. Hitchcock ◽  
Frank J. Bailey ◽  
Andrew A. Crimes ◽  
David A. G. Dean ◽  
Paul J. Davis
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Assay Procedure ◽  
Soya Proteins

Improved Enzyme-Linked Immunosorbent Assay for Determination of Soy Protein in Meat Products

1987 ◽  
Vol 70 (3) ◽  
pp. 582-587
Author(s):  
James H Rittenburg ◽  
Alexandra Adams ◽  
John Palmer ◽  
John C Allen
Keyword(s):  
Sample Preparation ◽  
Immunosorbent Assay ◽  
Soy Protein ◽  
Meat Products ◽  
Enzyme Linked Immunosorbent Assay ◽  
Liquid Form ◽  
Protein Levels ◽  
Wet Weight ◽  
Meat Samples

Abstract An Indirect, Competitive Enzyme-Linked Immunosorbent Assay (Elisa) Has Been Developed For Quantitation Of Soy Protein In Meat Products. The Methodology Allows Rapid Aqueous Extraction Of Meat Samples Into A Liquid Form Suitable For Assay. The Assay Is Highly Specific For Soy Protein And Is Designed To Measure Soy Protein Levels Between 1 And 10% Of The Wet Weight Of The Sample. Standardized, Stabilized Reagents For Carrying Out The Procedure Are Commercially Available In A Kit. The Analysis, Including Sample Preparation, Can Be Completed Within A Workday, And The Actual Immunoassay In Less Than 60 Min.

Enzyme-Linked Immunosorbent Assay of Ochratoxin A in Barley

1983 ◽  
Vol 66 (6) ◽  
pp. 1481-1484 ◽  
Author(s):  
Michael R A Morgan ◽  
Ruth Mcnerney ◽  
Henry W-S Chan
Keyword(s):  
Sample Preparation ◽  
Ochratoxin A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Dilution ◽  
Assay Sensitivity ◽  
Double Antibody ◽  
Minimal Sample ◽  
Ochratoxin Α

Abstract A noncompetitive, double antibody enzyme-linked immunosorbent assay for ochratoxin A using microtitration plates has been developed and applied to samples of barley. The anti-ochratoxin A antiserum, which is used at high dilution, does not cross-react significantly with ochratoxin B or ochratoxin α, Assay sensitivity for determination of the toxin in barley samples is 60 ng/kg. Minimal sample preparation is required before assay.

SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

1987 ◽  
Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø
Keyword(s):  
Plasminogen Activator ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mean Values ◽  
Amino Terminal ◽  
Type Plasminogen Activator ◽  
Healthy Donors ◽  
Urokinase Type Plasminogen Activator ◽  
The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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