Analysis of complex phthalic acid based polyesters by the combination of size exclusion chromatography and matrix-assisted laser desorption/ionization mass spectrometry

2014 ◽  
Vol 808 ◽  
pp. 94-103 ◽  
Author(s):  
Nadine O. Pretorius ◽  
Karsten Rode ◽  
Jaylin M. Simpson ◽  
Harald Pasch
Keyword(s):  
Mass Spectrometry ◽  
Phthalic Acid ◽  
Laser Desorption ◽  
Size Exclusion ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Exclusion Chromatography ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Evaluation of Matrix-Assisted Laser Desorption/Ionization Mass spectrometry for second-Generation Lignin Analysis

2012 ◽  
Vol 7 ◽  
pp. ACI.S10799 ◽  
Author(s):  
Aurore Richel ◽  
Caroline Vanderghem ◽  
Mathilde Simon ◽  
Bernard Wathelet ◽  
Michel Paquot
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Size Exclusion ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Molecular Weights ◽  
Desorption Ionization ◽  
Maldi Tof ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions ( m/z 100-600) in the positive ion mode offers a “fingerprint” of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus ( Miscanthus x giganteus) and Switchgrass ( Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.

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