A superhydrophobic chip integrated with an array of medium reservoirs for long-term hanging drop spheroid culture

Author(s):  
Bangyong Sun ◽  
Yi Zhao ◽  
Weimin Wu ◽  
Qiang Zhao ◽  
Gang Li
2011 ◽  
Vol 14 (2) ◽  
pp. 313-323 ◽  
Author(s):  
Amy Y. Hsiao ◽  
Yi-Chung Tung ◽  
Chuan-Hsien Kuo ◽  
Bobak Mosadegh ◽  
Rachel Bedenis ◽  
...  

Author(s):  
Weeks C Andrew ◽  
Aden Bethany ◽  
Zhang Junlin ◽  
Kilbey S Michael ◽  
Janorkar Amol

The Analyst ◽  
2020 ◽  
Vol 145 (21) ◽  
pp. 6974-6980
Author(s):  
Juhwan Park ◽  
Hwisoo Kim ◽  
Je-Kyun Park

We developed a simple method for liquid handling in a hanging drop array chip for spheroid culture and analysis by integrating microfluidic channels operated by pushbuttons.


2016 ◽  
Vol 105 (2) ◽  
pp. 377-388 ◽  
Author(s):  
C. Andrew Weeks ◽  
Bethany Aden ◽  
Junlin Zhang ◽  
Anisha Singh ◽  
Raymond D. Hickey ◽  
...  

2012 ◽  
Vol 173 ◽  
pp. 908-913 ◽  
Author(s):  
Karina Ziółkowska ◽  
Radosław Kwapiszewski ◽  
Agnieszka Stelmachowska ◽  
Michał Chudy ◽  
Artur Dybko ◽  
...  

2017 ◽  
Vol 312 (3) ◽  
pp. C277-C285 ◽  
Author(s):  
Amy J. Jorgenson ◽  
Kyoung Moo Choi ◽  
Delphine Sicard ◽  
Karry M. J. Smith ◽  
Samantha E. Hiemer ◽  
...  

Recent studies have implicated the Hippo pathway and its transcriptional effectors YAP and TAZ as necessary for fibroblast activation and tissue fibrosis. To test the specific and sufficient roles for TAZ in driving autonomous fibroblast activation, we cultured NIH3T3 fibroblasts expressing a doxycycline-inducible nuclear-localized mutant of TAZ (TAZ4SA) in scaffold-free 3D hanging drop spheroids, or on matrices of specified mechanical rigidity. Control NIH3T3 fibroblasts formed spheroids in hanging drop culture that remained stable and neither increased nor decreased in size significantly over 15 days. In contrast, TAZ4SA-transduced fibroblasts grew robustly in spheroid culture, and expressed enhanced levels of genes encoding profibrotic soluble factors connective tissue growth factor (CTGF), endothelin-1 (Et-1), and plasminogen activator inhibitor 1 (PAI-1). However, TAZ4SA expression was unable to enhance expression of extracellular matrix (ECM)-encoding genes Col1a1, Col1a2, Col3a1, or Fn1 in spheroid culture. Micromechanical testing indicated that spheroids composed of either control or TAZ4SA-expressing cells were highly compliant and indistinguishable in mechanical properties. In fibroblasts cultured on 2D matrices of compliance similar to spheroids, TAZ4SA expression was able to enhance contractile force generation, but was unable to enhance ECM gene expression. In contrast, culture on stiff hydrogels potentiated TAZ4SA enhancement of ECM expression. TAZ4SA enhancement of Col1a1 expression on soft matrices was potentiated by TGF-β1, while on stiff matrices it was abrogated by inhibition of myocardin-related transcription factor, demonstrating context-dependent crosstalk of TAZ with these pathways. These findings demonstrate sufficiency of TAZ activation for driving fibroblast proliferation, contraction, and soluble profibrotic factor expression, and mechanical context-dependent crosstalk of TAZ with other pathways in regulating Col1a1 expression.


2018 ◽  
Vol 2 (7) ◽  
pp. 1800054 ◽  
Author(s):  
Elise A. Aeby ◽  
Patrick M. Misun ◽  
Andreas Hierlemann ◽  
Olivier Frey

2017 ◽  
Vol 38 (8) ◽  
pp. 1206-1216 ◽  
Author(s):  
Agnieszka Zuchowska ◽  
Karina Kwapiszewska ◽  
Michal Chudy ◽  
Artur Dybko ◽  
Zbigniew Brzozka

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