Nitric oxide production during the osteogenic differentiation of human periodontal ligament mesenchymal stem cells

The present work reports the effect of the long-term exposure bone marrow mesenchymal stem cells from chronic ischemic heart disease patients with erythropoietin on morpho-functional properties. The mesenchymal stem cells were grown under 3rd passage in medium supplemented with 33.4 IU/mL of recormon to study expression of adhesion molecules, erythropoietin receptor, cytokine receptor to common β-chain on surface, and proliferative potential (MTT test, CFU, cell cycle), migratory ability, nitric oxide production under growth factor deprivation, oxidative stress and hyperglycemia conditions, and autophagy activity, and ultrastructure changes in cells. The results showed that the long-term exposure mesenchymal stem cells with erythropoietin significantly increased proliferation, nitric oxide production and some adhesion molecules and receptors expression on surface of those cells. In addition, the erythropoietin long-term exposure with mesenchymal stem cells increases autophagy activity. The finding of this work indicates that erythropoietin could be used to augment cell resistance to adverse microenvironment condition.

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Polyamines Modulate Nitric Oxide Production andCox-2Gene Expression in Response to Mechanical Loading in Human Adipose Tissue-Derived Mesenchymal Stem Cells

Stem Cells ◽

10.1634/stemcells.2005-0625 ◽

2006 ◽

Vol 24 (10) ◽

pp. 2262-2269 ◽

Cited By ~ 37

Author(s):

Geuranne S. Tjabringa ◽

Peter S. Vezeridis ◽

Behrouz Zandieh-Doulabi ◽

Marco N. Helder ◽

Paul I.J.M. Wuisman ◽

...

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Mechanical Loading ◽

Human Adipose Tissue ◽

Nitric Oxide Production ◽

Oxide Production

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Genetically Engineered Mesenchymal Stem Cells that Overexpress ACE2 or Angiotensin‐(1–7) Show Enhanced Nitric‐Oxide Production

The FASEB Journal ◽

10.1096/fasebj.27.1_supplement.lb689 ◽

2013 ◽

Vol 27 (S1) ◽

Author(s):

Vinayak Shenoy ◽

Meng Liu ◽

Anandharajan Rathinasabapathy ◽

Yanfei Qi ◽

Michael J Katovich ◽

...

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Genetically Engineered ◽

Nitric Oxide Production ◽

Oxide Production

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Paracrine Effects of Mesenchymal Stem Cells-Conditioned Medium on Microglial Cytokines Expression and Nitric Oxide Production

NeuroImmunoModulation ◽

10.1159/000365483 ◽

2014 ◽

Vol 22 (4) ◽

pp. 233-242 ◽

Cited By ~ 21

Author(s):

Yin Yin Ooi ◽

S. Thameem Dheen ◽

Samuel Sam Wah Tay

Keyword(s):

Nitric Oxide ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Conditioned Medium ◽

Nitric Oxide Production ◽

Paracrine Effects ◽

Oxide Production

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Basal nitric oxide production is enhanced by hydraulic pressure in cultured human periodontal ligament fibroblasts

American Journal of Orthodontics and Dentofacial Orthopedics ◽

10.1016/s0889-5406(00)70168-5 ◽

2000 ◽

Vol 117 (4) ◽

pp. 474-478 ◽

Cited By ~ 9

Author(s):

Chie Nakago-Matsuo ◽

Toshihiko Matsuo ◽

Tadao Nakago

Keyword(s):

Nitric Oxide ◽

Periodontal Ligament ◽

Hydraulic Pressure ◽

Nitric Oxide Production ◽

Periodontal Ligament Fibroblasts ◽

Oxide Production ◽

Human Periodontal Ligament Fibroblasts

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Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino-terminal kinases (JNK) pathway under inflammatory conditions

Cell Proliferation ◽

10.1111/cpr.12369 ◽

2017 ◽

Vol 50 (6) ◽

pp. e12369 ◽

Cited By ~ 36

Author(s):

Yi Tang ◽

Lin Liu ◽

Pei Wang ◽

Donglei Chen ◽

Ziqiang Wu ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Periodontal Ligament ◽

Jnk Pathway ◽

Amino Terminal ◽

Inflammatory Conditions

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Two Transcripts of FBXO5 Promote Migration and Osteogenic Differentiation of Human Periodontal Ligament Mesenchymal Stem Cells

BioMed Research International ◽

10.1155/2018/7849294 ◽

2018 ◽

Vol 2018 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Lin Liu ◽

Kun Liu ◽

Yanzhe Yan ◽

Zhuangzhuang Chu ◽

Yi Tang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Tissue Regeneration ◽

Periodontal Ligament ◽

Periodontal Tissue ◽

Alizarin Red ◽

Differentiation Potential ◽

Alp Activity ◽

Periodontal Tissue Regeneration

Objectives. Enhanced migration and osteogenic differentiation of mesenchymal stem cells (MSCs) are beneficial for MSC-mediated periodontal tissue regeneration, a promising method for periodontitis treatment. FBXO5, a member of the F-box protein family, is involved in the osteogenic differentiation of MSCs. Here, we investigated the effect of FBXO5 on human periodontal ligament stem cells (hPDLSCs). Materials and Methods. hPDLSCs were isolated from periodontal ligament tissue. Lentivirus FBXO5 shRNA was used to silence FBXO5 expression. Two transcripts of FBXO5 were overexpressed and transduced into hPDLSCs via retroviral infection. Migration and osteogenic differentiation of hPDLSCs were evaluated using the scratch migration assay, alkaline phosphatase (ALP) activity, ALP staining, alizarin red staining, western blotting, and real-time polymerase chain reaction. Results. The expression of FBXO5 was upregulated after osteogenic induction in hPDLSCs. FBXO5 knockdown attenuated migration, inhibited ALP activity and mineralization, and decreased RUNX2, OSX, and OCN expression, while the overexpression of two transcript isoforms significantly accelerated migration, enhanced ALP activity and mineralization, and increased RUNX2, OSX, and OCN expression in hPDLSCs. Conclusions. Both isoforms of FBXO5 promoted the migration and osteogenic differentiation potential of hPDLSCs, which identified a potential target for improving periodontal tissue regeneration.

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