Detection of bacteria in mid-trimester amniotic fluids by gene amplification of bacterial 16s ribosomal DNA sequences, cloning and sequence analysis

2006 ◽  
Vol 195 (6) ◽  
pp. S171 ◽  
Author(s):  
Claudel Jean-Pierre ◽  
Steven Witkin ◽  
Maria Schneider ◽  
Iara Linhares ◽  
Daniel Skupski ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Gene Amplification ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
16S Ribosomal Dna

scholarly journals Comparison of 16S Ribosomal DNA Sequences of All Xanthomonas Species

1997 ◽  
Vol 47 (2) ◽  
pp. 328-335 ◽  
Author(s):  
L. HAUBEN ◽  
L. VAUTERIN ◽  
J. SWINGS ◽  
E. R. B. MOORE
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
16S Ribosomal Dna ◽  
Xanthomonas Species

scholarly journals A Comparison of 16S Ribosomal DNA Sequences from Five Isolates of Helicobacter pylori

1994 ◽  
Vol 44 (2) ◽  
pp. 320-323 ◽  
Author(s):  
B. W. ECKLOFF ◽  
R. P. PODZORSKI ◽  
B. C. KLINE ◽  
F. R. COCKERILL
Keyword(s):  
Helicobacter Pylori ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
16S Ribosomal Dna

scholarly journals Reevaluation of Streptococcus bovis Endocarditis Cases from 1975 to 1985 by 16S Ribosomal DNA Sequence Analysis

2002 ◽  
Vol 40 (10) ◽  
pp. 3848-3850 ◽  
Author(s):  
I. A. Herrero ◽  
M. S. Rouse ◽  
K. E. Piper ◽  
S. A. Alyaseen ◽  
J. M. Steckelberg ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Ribosomal Dna ◽  
Dna Sequence Analysis ◽  
Streptococcus Bovis ◽  
16S Ribosomal Dna ◽  
Ribosomal Dna Sequence

scholarly journals 16S Ribosomal DNA Typing for Identification of Pathogens in Patients with Bacterial Keratitis

1998 ◽  
Vol 36 (12) ◽  
pp. 3492-3496 ◽  
Author(s):  
C. Michele Knox ◽  
Vickey Cevellos ◽  
Deborah Dean
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Dna ◽  
Diagnostic Tests ◽  
Dna Typing ◽  
Cross Sectional Study ◽  
Bacterial Keratitis ◽  
Sectional Study ◽  
Cross Sectional ◽  
16S Ribosomal Dna ◽  
Sequencing Result

The identification of pathogens in patients with bacterial keratitis remains problematic because standard diagnostic tests are negative for 40 to 60% of patients. A cross-sectional study was undertaken to determine if PCR and sequence analysis of 16S ribosomal DNA (rDNA) could be used to detect bacterial pathogens in patients with keratitis. Corneal specimens were collected for culture and rDNA typing. Variable segments of each rDNA specimen were amplified by PCR, sequenced, and aligned with the sequences in GenBank. Eleven patients had microbiologically documented bacterial keratitis, while 17 patients had keratitis due to other causes. Nine (82%) of 11 bacterial keratitis patients were PCR positive; each sequencing result matched the culture results. Seventeen (100%) patients with nonbacterial keratitis were PCR negative. Our data suggest that 16S rDNA typing holds promise as a rapid alternative to culture for identifying pathogens in patients with bacterial keratitis.

scholarly journals Phylogenetic Position of Taylorella equigenitalis Determined by Analysis of Amplified 16S Ribosomal DNA Sequences

1993 ◽  
Vol 43 (3) ◽  
pp. 618-621 ◽  
Author(s):  
N. M. C. Bluemink-Pluym ◽  
L. Van Dijk ◽  
A. H. M. Van Vliet ◽  
J. W. B. Van Der Giessen ◽  
B. A. M. Van Der Zeijst
Keyword(s):  
Ribosomal Dna ◽  
Dna Sequences ◽  
Phylogenetic Position ◽  
Taylorella Equigenitalis ◽  
16S Ribosomal Dna
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