Optimization of mixotrophic cultivation of microalgae Chlorella sp. for biofuel production using response surface methodology

2015 ◽  
Vol 7 ◽  
pp. 45-50 ◽  
Author(s):  
Virginija Skorupskaite ◽  
Violeta Makareviciene ◽  
Donatas Levisauskas
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biofuel Production ◽  
Mixotrophic Cultivation ◽  
Chlorella Sp

scholarly journals Development of Sustainable Phycoremediation With the Help of Chlorella Pyrenoidosa for Textile Wastewater Analysis Using Response Surface Methodology

2020 ◽  
Author(s):  
Shubhangi Mishra ◽  
Pradeep Kumar Srivast ◽  
Virendra Singh ◽  
Monika Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Productivity ◽  
Textile Wastewater ◽  
Optimal Growth ◽  
Chlorella Pyrenoidosa ◽  
Biofuel Production ◽  
Aquatic Life ◽  
Chlorella Sp ◽  
Textile Wastewaters

Abstract The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be used for biofuel production by in-situ transesterification.

scholarly journals Development of Sustainable Phycoremediation with the help of Chlorella Pyrenoidosa for Textile Wastewater Analysis using Response Surface Methodology

2020 ◽  
Author(s):  
Shubhangi Mishra ◽  
Pradeep Srivast ◽  
Virendra Singh ◽  
Monika Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Productivity ◽  
Textile Wastewater ◽  
Optimal Growth ◽  
Chlorella Pyrenoidosa ◽  
Biofuel Production ◽  
Aquatic Life ◽  
Chlorella Sp ◽  
Textile Wastewaters

Abstract The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be further used for biofuel production by in-situ transesterification.

Application of response surface methodology for optimization of growth and lipids in Scenedesmus abundans using batch culture system

RSC Advances ◽  
2014 ◽  
Vol 4 (42) ◽  
pp. 22129-22140 ◽  
Author(s):  
Chelladurai Chellamboli ◽  
Muthiah Perumalsamy
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Batch Culture ◽  
Culture System ◽  
Fossil Fuels ◽  
Biofuel Production ◽  
Alternative Source ◽  
System P

Owing to an increased demand for fuel and the depletion of fossil fuels, an alternative source such as algae is currently being exploited for biofuel production.

scholarly journals Subcritical Water Extraction of Chlorella pyrenoidosa: Optimization through Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Selvakumar Thiruvenkadam ◽  
Shamsul Izhar ◽  
Yoshida Hiroyuki ◽  
Razif Harun
Keyword(s):  
Response Surface Methodology ◽  
Reaction Time ◽  
Response Surface ◽  
Reaction Temperature ◽  
Subcritical Water ◽  
Chlorella Pyrenoidosa ◽  
Water Extraction ◽  
Biofuel Production ◽  
Oil Yield ◽  
Subcritical Water Extraction

Subcritical water extraction (SCW) was used to extract oil from Chlorella pyrenoidosa. The operational factors such as reaction temperature, reaction time, and biomass loading influence the oil yield during the extraction process. In this study, response surface methodology was employed to identify the desired extraction conditions for maximum oil yield. Experiments were carried out in batch reactors as per central composite design with three independent factors including reaction temperature (170, 220, 270, 320, and 370°C), reaction time (1, 5, 10, 15, and 20 min), and biomass loading (1, 3, 5, 10, and 15%). A maximum oil yield of 12.89 wt.% was obtained at 320°C and 15 min, with 3% biomass loading. Sequential model tests showed the good fit of experimental data to the second-order quadratic model. This study opens the great potential of SCW to extract algal oil for use in algal biofuel production.

Optimization of light and temperature for growing Chlorella sp. using response surface methodology

1996 ◽  
Vol 10 (5) ◽  
Author(s):  
J.L. Garc�a S�nchez ◽  
J.A. S�nchez P�rez ◽  
F. Garc�a Camacho ◽  
J.M. Fern�ndez Sevilla ◽  
E. Molina Grima
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Chlorella Sp

scholarly journals Optimization of Chlorella Culture Conditions with Response Surface Methodology to Increase Biomass

2021 ◽  
Vol 20 (5) ◽  
Author(s):  
R. Kanimozhi ◽  
D. Arvind Prasath ◽  
R. Dhandapani ◽  
Santhosh Sigamani
Keyword(s):  
Response Surface Methodology ◽  
Light Intensity ◽  
Response Surface ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Optimal Conditions ◽  
Optimum Conditions ◽  
Accession Number ◽  
Microalgal Biomass ◽  
Chlorella Sp

Microalgae is gaining popularity as a major ingredient in nutrition supplements. To mass cultivate, it is imperative to improve the biomass yield hence optimization of cultures conditions becomes paramount. In this work, an attempt has been made to optimize the microalgal production using response surface methodology (RSM) and validate further the optimized parameters. The optimum conditions for the cultivation of Chlorella sp. KPU016 under optimized nutrient conditions were pH 8.2, the light intensity of 3100 lx, glycerol 1.44 g.L-1 (under pre-set conditions of 12 h lighting, the temperature at 27±1°C. With these RSM-driven optimum conditions, the yield of microalgal biomass achieved was 282.50 mg.L-1. For larger-scale microalgal harvesting, the validated optimal conditions can be inferred as the best for enhanced microalgal production. The isolate was partially sequenced and submitted to the NCBI database and the GenBank accession number is MZ348364.

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