Optimization of light and temperature for growing Chlorella sp. using response surface methodology

1996 ◽  
Vol 10 (5) ◽  
Author(s):  
J.L. Garc�a S�nchez ◽  
J.A. S�nchez P�rez ◽  
F. Garc�a Camacho ◽  
J.M. Fern�ndez Sevilla ◽  
E. Molina Grima
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Chlorella Sp

scholarly journals Development of Sustainable Phycoremediation With the Help of Chlorella Pyrenoidosa for Textile Wastewater Analysis Using Response Surface Methodology

2020 ◽  
Author(s):  
Shubhangi Mishra ◽  
Pradeep Kumar Srivast ◽  
Virendra Singh ◽  
Monika Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Productivity ◽  
Textile Wastewater ◽  
Optimal Growth ◽  
Chlorella Pyrenoidosa ◽  
Biofuel Production ◽  
Aquatic Life ◽  
Chlorella Sp ◽  
Textile Wastewaters

Abstract The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be used for biofuel production by in-situ transesterification.

scholarly journals Optimization of Chlorella Culture Conditions with Response Surface Methodology to Increase Biomass

2021 ◽  
Vol 20 (5) ◽  
Author(s):  
R. Kanimozhi ◽  
D. Arvind Prasath ◽  
R. Dhandapani ◽  
Santhosh Sigamani
Keyword(s):  
Response Surface Methodology ◽  
Light Intensity ◽  
Response Surface ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Optimal Conditions ◽  
Optimum Conditions ◽  
Accession Number ◽  
Microalgal Biomass ◽  
Chlorella Sp

Microalgae is gaining popularity as a major ingredient in nutrition supplements. To mass cultivate, it is imperative to improve the biomass yield hence optimization of cultures conditions becomes paramount. In this work, an attempt has been made to optimize the microalgal production using response surface methodology (RSM) and validate further the optimized parameters. The optimum conditions for the cultivation of Chlorella sp. KPU016 under optimized nutrient conditions were pH 8.2, the light intensity of 3100 lx, glycerol 1.44 g.L-1 (under pre-set conditions of 12 h lighting, the temperature at 27±1°C. With these RSM-driven optimum conditions, the yield of microalgal biomass achieved was 282.50 mg.L-1. For larger-scale microalgal harvesting, the validated optimal conditions can be inferred as the best for enhanced microalgal production. The isolate was partially sequenced and submitted to the NCBI database and the GenBank accession number is MZ348364.

scholarly journals Development of Sustainable Phycoremediation with the help of Chlorella Pyrenoidosa for Textile Wastewater Analysis using Response Surface Methodology

2020 ◽  
Author(s):  
Shubhangi Mishra ◽  
Pradeep Srivast ◽  
Virendra Singh ◽  
Monika Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Productivity ◽  
Textile Wastewater ◽  
Optimal Growth ◽  
Chlorella Pyrenoidosa ◽  
Biofuel Production ◽  
Aquatic Life ◽  
Chlorella Sp ◽  
Textile Wastewaters

Abstract The uncontrolled utilization for the textile products is increasing year by year resulting with the elevating wastewater generated from the textile industries, which makes it among the prevalent sources of critical environmental deteoration issue globally. Products obtained from the dyes used are the primary toxic product for aquatic life, they cause aesthetic pollution, eutrophication, perturbation and increase in BOD and COD in aquatic life. Three types of textile wastewaters (Acid Yellow dye, Acid orange dye and Basic pink dye) has been used for wastewater treatment and microalgal (Chlorella pyrenoidosa) biomass production. Nitrogen content in textile wastewaters is very less, hence urea is used as nitrogen source in wastewater. Optimal growth condition (Urea-0.4g/L, wastewater- 40%(v/v)) is developed through Response surface methodology (RSM). The biomass productivity for chlorella sp. is 1.2-1.5 g/L/day in textile wastewaters. The reduction efficiency of COD, Nitrate-N Ammonia-N, Phosphate-P, and Dye(color) removal for Chlorella is 90-95%, 75-85%, 90-98%, 65-74% and 40-65%.After harvesting the Biomass by flocculation method it can be further used for biofuel production by in-situ transesterification.

scholarly journals Formulation of a minimal nutritional medium for enhanced lipid productivity in Chlorella sp. and Botryococcus sp. using response surface methodology

2018 ◽  
Vol 77 (6) ◽  
pp. 1660-1672 ◽  
Author(s):  
Rashi Vishwakarma ◽  
Dolly Wattal Dhar ◽  
Sunil Pabbi
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Large Scale ◽  
Biomass Productivity ◽  
Biodiesel Production ◽  
Lipid Yield ◽  
Chlorella Sp ◽  
Nutritional Medium ◽  
Single Super Phosphate ◽  
Muriate Of Potash

Abstract Chlorella sp. MCC 7 and Botryococcus sp. MCC 31 were investigated to enable large-scale biodiesel production from minimal constituents in the growth medium. Response surface methodology (RSM) was used to maximise the biomass productivity and lipid yield using only nitrogen (N), phosphorus (P) and potassium (K) as urea, single super phosphate and muriate of potash. The optimum values were 0.42 g/L nitrogen; 0.14 g/L phosphorus and 0.22 g/L potassium for Chlorella sp.; and 0.46 g/L; 0.14 g/L and 0.25 g/L for Botryococcus sp. Lipid yield of 42% for Chlorella sp. and 52% in Botryococcus sp. was observed. An enhancement in lipid yield by approximately 55% for Chlorella sp. and 73% for Botryococcus sp. was registered as compared to original nutrient medium. Fourier transform infrared (FTIR) analysis of extracted lipids revealed characteristic bands for triglycerides. This study provided utilisation of a practicable nutrient recipe in the form of N, P, K input for enhanced lipid yield from the selected microalgal strains.

scholarly journals Optimization of lipids’ ultrasonic extraction and production from Chlorella sp. using response-surface methodology

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Bilel Hadrich ◽  
Ismahen Akremi ◽  
Mouna Dammak ◽  
Mohamed Barkallah ◽  
Imen Fendri ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ultrasonic Extraction ◽  
Chlorella Sp

scholarly journals Optimization of Batch Cultivation of Chlorella sp. Using Response Surface Methodology

2019 ◽  
pp. 1-14
Author(s):  
Sandra E. Ezeani ◽  
Gideon O. Abu
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Biomass Production ◽  
Nitrogen Concentration ◽  
Inoculum Size ◽  
Limiting Factors ◽  
Minimal Effect ◽  
Maximum Biomass ◽  
Chlorella Sp ◽  
Chlorophyll Production

Optimal biomass production from microalgae using the NPK 20:20:20 medium; a relatively cheaper and locally available medium has been identified as an important factor in the large-scale algal biomass production. In this study, various concentrations (0.3-0.7 g/l) of NPK 20:20:20 were considered as the source of nitrogen in the growth medium for Chlorella sp. Four independent parameters in algae culture (nitrogen concentration, pH, inoculum size and duration of the experiment at varying ranges were studied for maximum biomass and chlorophyll production. Response Surface Methodology (RSM) procedure result that nitrogen concentration and pH level are the dominant factors affecting biomass and chlorophyll production. Maximum biomass was achieved at 0.5 g/l N and 8.5 pH value. Higher N (0.8 g/l) and lower N (0.3 g/l) had minimal effect on biomass and chlorophyll production. There was a linear relationship between chlorophyll and biomass production while the residual nitrogen had an inverse relationship with biomass production. Nitrogen concentration and pH were shown to be limiting factors under the conditions of the study. The inoculum size and duration of the experiment had a minimal effect on biomass production. 

Sign in / Sign up

Export Citation Format

Share Document