scholarly journals Selective production of the acyclic monoterpene β-myrcene by microplantlet suspension cultures of the macrophytic marine red alga Ochtodes secundiramea under nutrient perfusion cultivation with bromide-free medium

2018 ◽  
Vol 36 ◽  
pp. 159-166 ◽  
Author(s):  
Jason Polzin ◽  
Gregory L. Rorrer
Keyword(s):  
Red Alga ◽  
Suspension Cultures ◽  
Free Medium ◽  
Perfusion Cultivation

Effects of Culture Conditions on Isoflavonoid Levels of Transformed and Non-Transformed Cultures of Lupinus -a Comparison of Suspension and Hairy Root Cultures

1991 ◽  
Vol 46 (9-10) ◽  
pp. 735-742 ◽  
Author(s):  
J. Berlin ◽  
C. Rügenhagen ◽  
M. Rippert ◽  
S. Erdogan
Keyword(s):  
Suspension Culture ◽  
Hairy Root ◽  
Root Culture ◽  
Culture Conditions ◽  
Suspension Cultures ◽  
Stress Factors ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Free Medium ◽  
Lupinus Polyphyllus

Abstract Some highly productive suspension and hairy root cultures were found among several trans­formed cultures of Lupinus polyphyllus and L. hartwegii. A transformed suspension culture Lupo 30150 and a root culture Luha 15834 containing the highest specific isoflavone glucoside content were characterized and compared with normal phytohormone-dependent lines with respect to product stability as well as to their responsiveness to external triggers, e.g. response to changes in the medium. While phytohormone-dependent suspension cultures lost their ini­tial ability to form increased levels of isoflavonoids on phytohormone-free medium, the trans­formed phytohorm one-independent suspension Lupo 30150 remained a highly productive line, despite the fact that its specific levels decreased to 60% of the initial values during several years in liquid medium. Highest stability of product patterns and levels were noted for the transformed root culture. Phytohorm ones had little effect on growth and isoflavonoid levels in suspension cultures, while they reduced both strongly in root cultures. In the presence of 2,4-D the root culture changed into an aggregated low producing suspension culture from which the root state was recovered on phytohorm one-free medium. As long as the root state was main­ tained, isoflavonoid levels could not be distinctly improved by media variation while specific isoflavonoid levels of suspensions were increased by stress factors such as phosphate depletion. When suspensions were transferred to fresh medium phenylalanine ammonia-lyase was greatly induced within 24 h, while the activity remained nearly unchanged in root cultures.

Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

1978 ◽  
Vol 36 (2) ◽  
pp. 310-311
Author(s):  
W. Liebrich
Keyword(s):  
Hela Cells ◽  
Calf Serum ◽  
Glass Tube ◽  
Serum Free Medium ◽  
Nacl Solution ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Serum Free ◽  
All Solutions ◽  
Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

Sign in / Sign up

Export Citation Format

Share Document