Serological status for N. caninum, bovine viral diarrhea virus, and infectious bovine rhinotracheitis virus at pregnancy testing and reproductive performance in beef herds

2005 ◽  
Vol 90 (3-4) ◽  
pp. 219-242 ◽  
Author(s):  
C.L. Waldner
Keyword(s):  
Reproductive Performance ◽  
Bovine Viral Diarrhea ◽  
Infectious Bovine Rhinotracheitis ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Serological Status ◽  
Pregnancy Testing ◽  
Beef Herds ◽  
Viral Diarrhea Virus ◽  
Infectious Bovine Rhinotracheitis Virus

INVESTIGATION ON BOVINE VIRAL DIARRHEA VIRUS AND INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS IN MONGOLIAN DAIRY FARMS

2017 ◽  
Vol 19 (3) ◽  
pp. 10-15
Author(s):  
Dulam Purevtseren ◽  
Erdenechimeg Dashzevge ◽  
Zhou Wei Guan Guan
Keyword(s):  
Dairy Cows ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Infectious Bovine Rhinotracheitis ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
One Step ◽  
Viral Diarrhea Virus ◽  
Infectious Bovine Rhinotracheitis Virus

In this study, 168 blood sera were collected from dairy cows in Selenge and Tuv aimags during 2013 - 2014. The ELISA was carried out for serological detection of antibodies and antigens to Bovine viral diarrhea virus (BVDV), antibodies to Infectious bovine rhinotracheitis virus (IBRV) in dairy cows in Mongolia. The ELISA results of antibodies of BVDV and antigen of BVDV showed that 86.9% and 3.57%, respectively. The seroprevalence of antibodies against IBRV was found to be 60.7%. In order to confirm of BVDV, One Step RT-PCR was performed in ELISA positive cattle serum samples using specific primer for BVDV. The results showed that 294 bp fragment was successfully amplified. Phylogenetic analysis revealed that the nucleotide sequences 5'UTR gene of the isolates belonged to the BVDV1 subtype. Four isolated virus samples were closely related to China, the another isolate was closely related to the Slovenia BVDV1 isolate.

scholarly journals Evaluation of a Single Dilution ELISA System for Detection of Seroconversion to Bovine Viral Diarrhea Virus, Bovine Respiratory Syncytial Virus, Parainfluenza-3 Virus, and Infectious Bovine Rhinotracheitis Virus: Comparison with Testing by Virus Neutralization and Hemagglutination Inhibition

1998 ◽  
Vol 10 (1) ◽  
pp. 43-48 ◽  
Author(s):  
D. A. Graham ◽  
J. McShane ◽  
K. A. Mawhinney ◽  
I. E. McLaren ◽  
B. M. Adair ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Infectious Bovine Rhinotracheitis ◽  
Elisa System ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Syncytial Virus ◽  
Viral Diarrhea Virus ◽  
Infectious Bovine Rhinotracheitis Virus

A single-dilution quantitative enzyme-linked immunosorbent assay (ELISA) system, based on commercial ELISA kits, for the simultaneous detection of seroconversion to bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus (BRSV), parainfluenza-3 virus (PI3V), and infectious bovine rhinotracheitis virus (IBRV) was evaluated by testing acute and convalescent serum pairs from 564 cattle in 145 outbreaks of respiratory disease. Seroconversion to BVDV, BRSV, PI3V and IBRV was detected in 8.0%, 19.0%, 13.7%, and 7.4%, respectively, of serum pairs tested. Seroconversion was detected in 60.7% of herds and 34.6% of animals tested. Infection with 2 or more viruses was found in 46.6% of these herds and in 27.2% of these animals. The majority of BVDV infections (62%) were associated with other virus infections, suggesting that BVDV may potentiate infection with other agents rather than being a primary pathogen of the respiratory tract. The results were compared with those obtained by virus neutralization and hemagglutination inhibition testing, and the sensitivity, specificity, and overall correlation were calculated. Sensitivities of 92%, 95%, 100%, and 100% were obtained for BVDV, BRSV, PI3V, and IBRV, respectively. The corresponding specificity values were 89%, 92%, 86%, and 91%. The overall correlation for each virus was 90%, 93%, 90%, and 93%, respectively. These results demonstrate that this ELISA system may be used successfully to detect seroconversion in serum pairs, highlight the frequency of multiple viral infections in outbreaks of respiratory disease, and provide further evidence of an immunosuppressive role for BVDV infections.

scholarly journals Standardization of Enzyme-Linked Immunosorbent Assays (ELISAs) for Quantitative Estimation of Antibodies Specific for Infectious Bovine Rhinotracheitis Virus, Respiratory Syncytial Virus, Parainfluenza-3 Virus, and Bovine Viral Diarrhea Virus

1997 ◽  
Vol 9 (1) ◽  
pp. 24-31 ◽  
Author(s):  
D. A. Graham ◽  
K. A. Mawhinney ◽  
J. McShane ◽  
T. J. Connor ◽  
B. M. Adair ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Bovine Viral Diarrhea Virus ◽  
Bovine Viral Diarrhea ◽  
Infectious Bovine Rhinotracheitis ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Syncytial Virus ◽  
Viral Diarrhea Virus ◽  
Infectious Bovine Rhinotracheitis Virus

Commercial enzyme-linked immunosorbent assays (ELISAs) for detection of serum antibodies to bovine viral diarrhea virus (BVDV), parainfluenza-3 virus (PI3V), respiratory syncytial virus (RSV), and infectious bovine rhinotracheitis virus (IBRV) were standardized to give a quantitative result when testing was performed at a single optimum dilution. For each test, serum samples were titrated and their end point titers calculated by an algebraic method directly from a plot of each titration series and also from a regression line fitted to this plot. The corrected optical density (COD) of each sample when tested at dilutions of 1/25, 1/50, and 1/100 was expressed as a percentage of the COD of a positive reference serum included on each plate, this value was the sample/positive (S/P) ratio. For each test, the linear relationship between the S/P ratio obtained at a dilution of 1/25, 1/50, and 1/100 and the end point titer calculated by each method was determined. In each case, the best linear relationship existed when samples were tested at a dilution of 1/100 ( r = 0.973 for BVDV, 0.962 for PI3V, 0.961 for RSV, 0.947 for IBRV). From the equation of these lines, an increase in the S/P ratio between acute and convalescent serum samples of 31%, 23%, 21%, and 35% would correspond to a 4-fold rise in ELISA titer to BVDV, PI3V, RSV, and IBRV, respectively. ELISA titers calculated from S/P ratios at 1/100 were significantly related to virus neutralization titers to BVDV, RSV, and IBRV and to hemagglutination inhibition titers to PI3V ( P ≪ 0.001 in all cases). Samples with low S/P ratios had the greatest intraassay and interassay variation. Intraassay reproducibility ranged from 3.5% to 22.3% (coefficient of variation), with a median value of 9.5%. Interassay reproducibility was lower, ranging from 6.0% to 50.6%, with a median of 17.4%.

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