Inhibitory effect of mycophenolic acid on the replication of infectious pancreatic necrosis virus and viral hemorrhagic septicemia virus

2008 ◽  
Vol 80 (3) ◽  
pp. 332-338 ◽  
Author(s):  
L MARROQUI ◽  
A ESTEPA ◽  
L PEREZ
Keyword(s):  
Mycophenolic Acid ◽  
Pancreatic Necrosis ◽  
Inhibitory Effect ◽  
Infectious Pancreatic Necrosis Virus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Necrosis Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

scholarly journals Detections of Rainbow Trout Antibodies to Infectious Pancreatic Necrosis Virus and Viral Hemorrhagic Septicemia Virus via a Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Cuneyt TAMER ◽  
Semra GUMUSOVA ◽  
Harun ALBAYRAK
Keyword(s):  
Rainbow Trout ◽  
Recombinant Protein ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Infectious Pancreatic Necrosis Virus (IPNV) and Viral hemorrhagic septicemia virus (VHSV) cause significant losses in the aquaculture industry. There have been few reports of the use of screening rainbow trout for antibodies against IPNV and VHSV as an epidemiological tool. Several ELISAs using a whole virus or recombinant IPNV and VHSV proteins have been described. In this study, a recombinant protein-based enzyme-linked immunosorbent assay (ELISA) for the detection of IPNV and VHSV antibodies in rainbow trout (Oncorhynchus mykiss) was evaluated. To develop recombinant protein-based enzyme-linked immunosorbent assays, a fragment containing the entire length of the gG gene of VHSV and VP2 of IPNV was amplified by PCR using the viruses' genomic RNA and cloned in pET-28a(+) plasmid. Recombinant structural viral proteins (rVP2 and rgG) were expressed in the Escherichia coli BL21 (DE3). The rgG was extracted and purified. 96-well plates were coated with VP2 and gG separately. For VHSV, Assay could detect until 1/15625 dilution in VHSV positive fish serum. For IPNV, Assays could detect until 1/3125 dilution in IPNV positive fish serum. These results show rgG and rVP2, used in ELISA, are more sensitive than virus neutralization tests.

Infectious Pancreatic Necrosis Virus in Young Salmonids of the Canadian Maritime Provinces

1969 ◽  
Vol 26 (12) ◽  
pp. 3259-3262 ◽  
Author(s):  
R. M. MacKelvie ◽  
H. Artsob
Keyword(s):  
Tissue Culture ◽  
Atlantic Salmon ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Fish Culture ◽  
Necrosis Virus ◽  
Maritime Provinces ◽  
Viral Hemorrhagic Septicemia ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Infectious pancreatic necrosis virus was isolated from young salmonids, including Atlantic salmon (Salmo solar), in 9 of the 13 fish culture stations of the Canadian Maritime Provinces. The virus was isolated in tissue culture and identified serologically. There was no evidence of Viral Hemorrhagic Septicemia (Egtved disease).

Role of infectious pancreatic necrosis virus (IPNV) infection in co-infections with other viruses

2018 ◽  
Vol 74 (1) ◽  
pp. 6050-2018
Author(s):  
JOANNA MAJ-PALUCH ◽  
MICHAŁ REICHERT
Keyword(s):  
Defense Mechanisms ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Infectious Salmon Anaemia Virus ◽  
Necrosis Virus ◽  
Infectious Hematopoietic Necrosis ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Co-infection is an infection of more than one pathogen. In aquatic environment, the most common occurrence is appearance of infectious pancreatic necrosis virus (IPNV) in the presence of other viruses such as infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious salmon anaemia virus (ISAV), or salmonid alphavirus (SAV). In most cases, the IPN virus reduces the proliferation of other viruses in cell cultures or in the internal organs of salmonids, for example in IHNV or ISAV co-infections. However, it happens that there is no significant effect on the multiplication of the virus with which it coexists, e.g. IPNV-VHSV. Body's defense mechanisms, interferon and other interferon-like factors or mutations in the genome play an important role in co-infection..

scholarly journals Role of infectious pancreatic necrosis virus (IPNV) infection in co-infections with other viruses

2018 ◽  
Vol 74 (4) ◽  
pp. 243-246
Author(s):  
JOANNA MAJ-PALUCH ◽  
MICHAŁ REICHERT
Keyword(s):  
Defense Mechanisms ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Infectious Salmon Anaemia Virus ◽  
Necrosis Virus ◽  
Infectious Hematopoietic Necrosis ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Co-infection is an infection of more than one pathogen. In an aquatic environment, the most common occurrence is the appearance of infectious pancreatic necrosis virus (IPNV) in the presence of other viruses such as infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious salmon anaemia virus (ISAV), or salmonid alphavirus (SAV). In most cases, the IPN virus reduces the proliferation of other viruses in cell cultures or in the internal organs of salmonids; for example, in IHNV or ISAV co-infections. However, it also happens that there is no significant effect on the multiplication of the virus with which it coexists, e.g. IPNV-VHSV. A body's defense mechanisms, interferon and other interferon-like factors or mutations in the genome play an important role in co-infection..

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