Rapid determination of antiviral drug susceptibility of human cytomegalovirus by real-time PCR

2009 ◽  
Vol 81 (1) ◽  
pp. 64-67 ◽  
Author(s):  
Nathalie Schnepf ◽  
Nicolas Boiteau ◽  
Françoise Petit ◽  
Sophie Alain ◽  
Marie-José Sanson-Le Pors ◽  
...  
Keyword(s):  
Real Time ◽  
Human Cytomegalovirus ◽  
Real Time Pcr ◽  
Rapid Determination ◽  
Antiviral Drug ◽  
Drug Susceptibility ◽  
Antiviral Drug Susceptibility

scholarly journals Application of Real-Time PCR for Determination of Antiviral Drug Susceptibility of Herpes Simplex Virus

2002 ◽  
Vol 46 (9) ◽  
pp. 2943-2947 ◽  
Author(s):  
Růžena Stránská ◽  
Anton M. van Loon ◽  
Merjo Polman ◽  
Rob Schuurman
Keyword(s):  
Herpes Simplex ◽  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Drug ◽  
Drug Susceptibility ◽  
Pcr Assay ◽  
Simplex Virus ◽  
Antiviral Drug Susceptibility ◽  
Hsv 1

ABSTRACT A quantitative real-time PCR (TaqMan) assay was developed for determination of antiviral drug susceptibility of herpes simplex virus (HSV). After short-time culture of the virus, the antiviral drug susceptibility of HSV isolates for acyclovir (ACV) was determined by measuring the reduction of the HSV type 1 (HSV-1) DNA levels in culture supernatants using real-time PCR. The 50% inhibitory concentration was reported as the concentration of antiviral drug that reduced the number of HSV-1 DNA copies by 50%. A total of 15 well-characterized ACV-sensitive or -resistant strains and clinical isolates were used for assay evaluation. The new assay with real-time PCR readout permitted rapid (3 days), objective, and reproducible determination of HSV-1 drug susceptibilities with no need for stringent control of initial multiplicity of infection. Furthermore, the real-time PCR assay results showed good correlation (r = 0.86) with those for the plaque reduction assay. In conclusion, the real-time PCR assay described here is a suitable quantitative method for determination of antiviral susceptibility of HSV-1, amenable for use in the routine diagnostic virology laboratory.

Real-time PCR determination of human herpesvirus 6 antiviral drug susceptibility

2007 ◽  
Vol 140 (1-2) ◽  
pp. 25-31 ◽  
Author(s):  
Yuji Isegawa ◽  
Masaya Takemoto ◽  
Koichi Yamanishi ◽  
Atsushi Ohshima ◽  
Nakaba Sugimoto
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Drug ◽  
Human Herpesvirus ◽  
Drug Susceptibility ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6 ◽  
Antiviral Drug Susceptibility

scholarly journals Real-Time PCR as a Versatile Tool for Investigating the Susceptibility of Human Herpesvirus 6 to Antiviral Agents

2003 ◽  
Vol 47 (9) ◽  
pp. 3021-3024 ◽  
Author(s):  
Muriel Macé ◽  
Chaysavanh Manichanh ◽  
Pascale Bonnafous ◽  
Stéphanie Précigout ◽  
David Boutolleau ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
Human Herpesvirus ◽  
Drug Susceptibility ◽  
Human Herpesvirus 6 ◽  
Versatile Tool ◽  
Herpesvirus 6

ABSTRACT A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.

Real time PCR for rapid determination of susceptibility of adenovirus to antiviral drugs

2010 ◽  
Vol 164 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Rosana Gainotti ◽  
Carmen Ricarte ◽  
Beatriz Ebekian ◽  
Cristina Videla ◽  
Guadalupe Carballal ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Determination ◽  
Antiviral Drugs

Rapid determination of adenoviral vector titers by quantitative real-time PCR

2001 ◽  
Vol 93 (1-2) ◽  
pp. 181-188 ◽  
Author(s):  
Libin Ma ◽  
Hans A.R. Bluyssen ◽  
Marc De Raeymaeker ◽  
Veerle Laurysens ◽  
Niels van der Beek ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Adenoviral Vector ◽  
Rapid Determination ◽  
Quantitative Real Time Pcr

Rapid determination of HHV-6 viral load by real-time PCR

2002 ◽  
Vol 44 (2) ◽  
pp. 116
Author(s):  
G.J.A. Eltringham ◽  
A.M. Kearns ◽  
A. Turner ◽  
C. Taylor ◽  
R. Freeman ◽  
...  
Keyword(s):  
Viral Load ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Determination

A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus

2007 ◽  
Vol 68 (2) ◽  
pp. 376-384 ◽  
Author(s):  
Michael C.L. Vickery ◽  
William B. Nilsson ◽  
Mark S. Strom ◽  
Jessica L. Nordstrom ◽  
Angelo DePaola
Keyword(s):  
16S Rrna ◽  
Real Time ◽  
Real Time Pcr ◽  
Vibrio Vulnificus ◽  
Rapid Determination ◽  
Pcr Assay
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