scholarly journals Real-Time PCR as a Versatile Tool for Investigating the Susceptibility of Human Herpesvirus 6 to Antiviral Agents

2003 ◽  
Vol 47 (9) ◽  
pp. 3021-3024 ◽  
Author(s):  
Muriel Macé ◽  
Chaysavanh Manichanh ◽  
Pascale Bonnafous ◽  
Stéphanie Précigout ◽  
David Boutolleau ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Agents ◽  
Antiviral Drug ◽  
Human Herpesvirus ◽  
Drug Susceptibility ◽  
Human Herpesvirus 6 ◽  
Versatile Tool ◽  
Herpesvirus 6

ABSTRACT A quantitative real-time PCR assay was developed for the determination of antiviral drug susceptibility and growth kinetics of human herpesvirus 6. The susceptibility and fitness of a sensitive strain, HST, and its ganciclovir-resistant derivative, GCVR1, were then characterized, leading us to conclude that the mutations of this latter virus did not alter its fitness significantly.

Real-time PCR determination of human herpesvirus 6 antiviral drug susceptibility

2007 ◽  
Vol 140 (1-2) ◽  
pp. 25-31 ◽  
Author(s):  
Yuji Isegawa ◽  
Masaya Takemoto ◽  
Koichi Yamanishi ◽  
Atsushi Ohshima ◽  
Nakaba Sugimoto
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Drug ◽  
Human Herpesvirus ◽  
Drug Susceptibility ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6 ◽  
Antiviral Drug Susceptibility

Identification of human herpesvirus 6 variants A and B by primer-specific real-time PCR may help to revisit their respective role in pathology

2006 ◽  
Vol 35 (3) ◽  
pp. 257-263 ◽  
Author(s):  
David Boutolleau ◽  
Caroline Duros ◽  
Pascale Bonnafous ◽  
Delphine Caïola ◽  
Alexandre Karras ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Respective Role ◽  
Herpesvirus 6

Persistence of DNA in cell cultures may jeopardize the analysis of human herpesvirus 6 dynamics by means of real-time PCR

2005 ◽  
Vol 125 (1) ◽  
pp. 95-98 ◽  
Author(s):  
Pascale Bonnafous ◽  
Agnès Gautheret-Dejean ◽  
David Boutolleau ◽  
Delphine Caïola ◽  
Henri Agut
Keyword(s):  
Real Time ◽  
Cell Cultures ◽  
Real Time Pcr ◽  
Human Herpesvirus ◽  
Human Herpesvirus 6 ◽  
Herpesvirus 6 ◽  
Persistence Of Dna

scholarly journals Application of Real-Time PCR for Determination of Antiviral Drug Susceptibility of Herpes Simplex Virus

2002 ◽  
Vol 46 (9) ◽  
pp. 2943-2947 ◽  
Author(s):  
Růžena Stránská ◽  
Anton M. van Loon ◽  
Merjo Polman ◽  
Rob Schuurman
Keyword(s):  
Herpes Simplex ◽  
Real Time ◽  
Real Time Pcr ◽  
Antiviral Drug ◽  
Drug Susceptibility ◽  
Pcr Assay ◽  
Simplex Virus ◽  
Antiviral Drug Susceptibility ◽  
Hsv 1

ABSTRACT A quantitative real-time PCR (TaqMan) assay was developed for determination of antiviral drug susceptibility of herpes simplex virus (HSV). After short-time culture of the virus, the antiviral drug susceptibility of HSV isolates for acyclovir (ACV) was determined by measuring the reduction of the HSV type 1 (HSV-1) DNA levels in culture supernatants using real-time PCR. The 50% inhibitory concentration was reported as the concentration of antiviral drug that reduced the number of HSV-1 DNA copies by 50%. A total of 15 well-characterized ACV-sensitive or -resistant strains and clinical isolates were used for assay evaluation. The new assay with real-time PCR readout permitted rapid (3 days), objective, and reproducible determination of HSV-1 drug susceptibilities with no need for stringent control of initial multiplicity of infection. Furthermore, the real-time PCR assay results showed good correlation (r = 0.86) with those for the plaque reduction assay. In conclusion, the real-time PCR assay described here is a suitable quantitative method for determination of antiviral susceptibility of HSV-1, amenable for use in the routine diagnostic virology laboratory.

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