scholarly journals Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity

2016 ◽  
Vol 61 (1) ◽  
pp. 65-75 ◽  
Author(s):  
M.G. Shehata ◽  
S.A. El Sohaimy ◽  
Malak A. El-Sahn ◽  
M.M. Youssef
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Cholesterol Lowering ◽  
Probiotic Lactic Acid Bacteria ◽  
Bile Salt Hydrolase Activity

Relationship between bile salt hydrolase activity, changes in the internal pH and tolerance to bile acids in lactic acid bacteria

2012 ◽  
Vol 34 (8) ◽  
pp. 1511-1518 ◽  
Author(s):  
Ana Yanina Bustos ◽  
Lucila Saavedra ◽  
Graciela Font de Valdez ◽  
Raúl Ricardo Raya ◽  
María Pía Taranto
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Bile Acids ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Internal Ph ◽  
Bile Salt Hydrolase Activity

scholarly journals Testing of selected probiotic properties of lactic acid bacteria isolated from vegetarians and meat-eaters faeces

2014 ◽  
Vol 7 (1) ◽  
pp. 38-43
Author(s):  
Eva Hybenová ◽  
Lucia Birošová ◽  
Kristína Nagyová ◽  
Júlia Štofirová ◽  
Nikoleta Šaková ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Ph Value ◽  
Bacterial Species ◽  
Low Ph ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Probiotic Properties ◽  
Bile Salt Hydrolase Activity

Abstract The aim of this work was to evaluate presence and properties of lactic acid bacteria in the faeces of 240 volunteers with various nutrition habits (vegetarians versus meat-eaters). Lactic acid bacteria counts in all age groups were nearly 5 or 6 logarithmic orders. Significantly higher amounts were found in women. Subsequently, based on the age and dietary pattern of probands, four samples were selected for isolation of lactic acid bacteria and identification of isolates in order to assign them to bacterial species. About 80 lactic acid bacteria were isolated from the faeces of young (21-30 years) and older (51-60 years) vegetarians and meateaters. The identification of the isolates was based on their morphological and biochemical characteristics. Isolates belong to lactobacilli, bifidobacteria, enterococci and propionibacteria. Surprisingly, bifidobacteria were predominated in older age group. The following probiotic properties were determined: survival at low pH value, and bile salt hydrolase activity. All strains were negative in bile salt hydrolase activity, but their growth was not inhibited in the presence of bile. The results from the study of survival at low pH value showed considerable variability in both dietary groups regardless the age of probands. However, it can be concluded, that bacteria isolated from the samples of older probands were more sensitive to acid pH.

scholarly journals Screening of Lactic Acid Bacteria for Bile Salt Hydrolase Activity

1999 ◽  
Vol 82 (12) ◽  
pp. 2530-2535 ◽  
Author(s):  
H. Tanaka ◽  
K. Doesburg ◽  
T. Iwasaki ◽  
I. Mierau
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Bile Salt Hydrolase Activity

scholarly journals Probiotic Properties and Bile Salt Hydrolase Activity of Some Isolated Lactic Acid Bacteria

2017 ◽  
Vol 0 (0) ◽  
pp. 87-100
Author(s):  
Asmaa Negm El-Dein ◽  
Azza Noor El-Deen ◽  
Sahar Tolba ◽  
Einas El-Shatoury ◽  
Ghada Awad ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Hydrolase Activity ◽  
Bile Salt Hydrolase ◽  
Probiotic Properties ◽  
Bile Salt Hydrolase Activity

scholarly journals Cholesterol-Lowering Potentials of Lactic Acid Bacteria Based on Bile-Salt Hydrolase Activity and Effect of Potent Strains on Cholesterol MetabolismIn VitroandIn Vivo

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Cheng-Chih Tsai ◽  
Pei-Pei Lin ◽  
You-Miin Hsieh ◽  
Zi-yi Zhang ◽  
Hui-Ching Wu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Bile Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Hepg2 Cells ◽  
Bile Salt Hydrolase ◽  
Apo B ◽  
Probiotic Strains ◽  
Bile Salt Hydrolase Activity ◽  
Bsh Activity

This study collected different probiotic isolates from animal and plant sources to evaluate the bile-salt hydrolase activity of probioticsin vitro. The deconjugation potential of bile acid was determined using high-performance liquid chromatography. HepG2 cells were cultured with probiotic strains with high BSH activity. The triglyceride (TG) and apolipoprotein B (apo B) secretion by HepG2 cells were evaluated. Our results show that the BSH activity and bile-acid deconjugation abilities ofPediococcus acidilacticiNBHK002,Bifidobacterium adolescentisNBHK006,Lactobacillus rhamnosusNBHK007, andLactobacillus acidophilusNBHK008 were higher than those of the other probiotic strains. The cholesterol concentration in cholesterol micelles was reduced within 24 h. NBHK007 reduced the TG secretion by 100% after 48 h of incubation. NBHK002, NBHK006, and NBHK007 could reduce apo B secretion by 33%, 38%, and 39%, respectively, after 24 h of incubation. The product PROBIO S-23 produced a greater decrease in the total concentration of cholesterol, low-density lipoprotein, TG, and thiobarbituric acid reactive substance in the serum or livers of hamsters with hypercholesterolemia compared with that of hamsters fed with a high-fat and high-cholesterol diet. These results show that the three probiotic strains of lactic acid bacteria are better candidates for reducing the risk of cardiovascular disease.

Screening of Bile Salt Hydrolase-Active Lactic Acid Bacteria for Potential Cholesterol-Lowering Probiotic Use

2011 ◽  
Vol 345 ◽  
pp. 139-146 ◽  
Author(s):  
Chun Feng Guo ◽  
Lan Wei Zhang ◽  
Jing Yan Li ◽  
Ying Chun Zhang ◽  
Chao Hui Xue ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Enterococcus Faecium ◽  
Lactobacillus Casei ◽  
Acid Resistance ◽  
Bile Salt Hydrolase ◽  
Cholesterol Lowering ◽  
Reduce Serum Cholesterol ◽  
Bile Salt Deconjugation

.Cholesterol-lowering effect of lactic acid bacteria (LAB) with bile salt hydrolase activity is well known. In this study, 150 LAB were screened for bile salt deconjugation ability and probiotic characters. Fourteen isolates with higher bile salt deconjugation ability were initially screened out using deconjugation rate above 50% as standard. These isolates were further screened for adhesion to HT-29 cells, bile tolerance and acid resistance. Four isolates, namely Lactobacillus casei F0822, Lactobacillus casei F0422, Enterococcus faecium F0511 and Enterococcus faecium IN7.12, was finally screened out. The 4 isolates may be able to reduce serum cholesterol levels in human and thus have a potential to apply in the biomedicine field.

scholarly journals STUDI VIABILITAS ISOLAT BAKTERI ASAM LAKTAT YANG DIISOLASI DARI ASINAN REBUNG BAMBU TABAH TERHADAP pH RENDAH DAN GARAM EMPEDU

2019 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Nurul Octavia Wasis ◽  
Nyoman Semadi Antara ◽  
Ida Bagus Wayan Gunam
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Bile Salts ◽  
Salt Resistance ◽  
Low Ph ◽  
Fermented Food ◽  
Bamboo Shoot ◽  
Probiotic Lactic Acid Bacteria ◽  
Mrs Broth

Tabah bamboo shoot pickle is one of the fermented food which is the source of lactic acid bacteria.  Lactic acid bacteria (LAB) is beneficial to health because it has the ability as a probiotic. Lactic acid bacteria that have probiotic criteria should have resistance to low pH and bile salts. This study aims to determine isolates of lactic acid bacteria isolated from tabah bamboo shoot pickle resistant to low pH and bile salts (NaDC). Lactic acid bacteria were tested to low pH by using MRS broth that have different pH (pH 2, pH 3, pH 4 and pH 6.2 as a control) incubated at 37ºC for 3 hours. isolates were survive in low pH then continued in bile salt resistance test with 0.3% bile salt concentration for 15 minutes, 30 minutes, 45 minutes, 60 minutes and 24 hours. The results showed that three isolates out of 88 isolates had ability to grow in low pH and in medium supplemented by NaDC 0,3%. The isolates are AR 3057, AR 3101 and AR 6152 which can be used as candidat of  probiotic. Keywords : Tabah bamboo shoot pickle, lactic acid bacteria, probiotic, low pH, bile salt

scholarly journals Isolation, Identification, and Evaluation of New Lactic Acid Bacteria Strains with Both Cellular Antioxidant and Bile Salt Hydrolase Activities In Vitro

2016 ◽  
Vol 79 (11) ◽  
pp. 1919-1928 ◽  
Author(s):  
SHUANG XU ◽  
TAIGANG LIU ◽  
CHIRAZ AKOREDE IBINKE RADJI ◽  
JING YANG ◽  
LANMING CHEN
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Genomic Diversity ◽  
Bile Salt Hydrolase ◽  
Fermented Foods ◽  
Intact Cells

ABSTRACT In this study, we analyzed Chinese traditional fermented food to isolate and identify new lactic acid bacteria (LAB) strains with novel functional properties and to evaluate their cellular antioxidant and bile salt hydrolase (BSH) activities in vitro. A sequential screening strategy was developed to efficiently isolate and obtain 261 LAB strains tolerant of bile salt, acid, and H2O2 from nine Chinese traditional fermented foods. Among these strains, 70 were identified as having 2,2-diphenyl-1-picrylhydrazyl radical scavenging and/or BSH activity. These strains belonged to eight species: Enterococcus faecium (33% of the strains), Lactobacillus plantarum (26%), Leuconostoc mesenteroides (14%), Pediococcus pentosaceus (6%), Enterococcus durans (9%), Lactobacillus brevis (9%), Pediococcus ethanolidurans (3%), and Lactobacillus casei (1%). The pulsed-field gel electrophoresis genome fingerprinting profiles of these strains revealed 38 distinct pulsotypes, indicating a high level of genomic diversity among the tested strains. Twenty strains were further evaluated for hydroxyl radical scavenging activity, reducing power, and ferrous ion chelating activity exerted by both viable intact cells and/or intracellular cell-free extracts. Some strains, such as L. plantarum D28 and E. faecium B28, had high levels of both cellular antioxidant and BSH activities in vitro. These strains are promising probiotic components for health-promoting functional foods.

Sign in / Sign up

Export Citation Format

Share Document