Isolation and characterization of two cytochrome P450 aromatase forms in killifish (Fundulus heteroclitus): Differential expression in fish from polluted and unpolluted environments

2005 ◽  
Vol 71 (4) ◽  
pp. 371-389 ◽  
Author(s):  
Sarah R. Greytak ◽  
Denise Champlin ◽  
Gloria V. Callard
Keyword(s):  
Cytochrome P450 ◽  
Differential Expression ◽  
Fundulus Heteroclitus ◽  
P450 Aromatase ◽  
Isolation And Characterization ◽  
Cytochrome P450 Aromatase

scholarly journals Characterization of Two Types of Cytochrome P450 Aromatase in the Serial-sex Changing Gobiid Fish, Trimma okinawae

2004 ◽  
Vol 21 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Yasuhisa Kobayashi ◽  
Tohru Kobayashi ◽  
Masaru Nakamura ◽  
Tomoki Sunobe ◽  
Craig E. Morrey ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Gobiid Fish ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase

scholarly journals Biophysical characterization of Aptenodytes forsteri cytochrome P450 aromatase

2018 ◽  
Vol 184 ◽  
pp. 79-87
Author(s):  
Francisco Zarate-Perez ◽  
Jesús B. Velázquez-Fernández ◽  
Gareth K. Jennings ◽  
Lisa S. Shock ◽  
Charles E. Lyons ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Biophysical Characterization ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase ◽  
Aptenodytes Forsteri

Isolation and characterization of the cDNA encoding the tilapia (Oreochromis niloticus) cytochrome P450 aromatase (P450arom): changes in P450arom mRNA, protein and enzyme activity in ovarian follicles during oogenesis

1997 ◽  
Vol 18 (1) ◽  
pp. 57-66 ◽  
Author(s):  
X T Chang ◽  
T Kobayashi ◽  
H Kajiura ◽  
M Nakamura ◽  
Y Nagahama
Keyword(s):  
Cytochrome P450 ◽  
Amino Acid ◽  
Oreochromis Niloticus ◽  
Ovarian Follicle ◽  
Ovarian Follicles ◽  
Aromatase Activity ◽  
Western Blots ◽  
P450 Aromatase ◽  
Isolation And Characterization ◽  
Cytochrome P450 Aromatase

ABSTRACT A cDNA clone encoding the complete tilapia (a teleost fish, Oreochromis niloticus) cytochrome P450 aromatase (P450arom) was isolated from an ovarian follicle cDNA library. The deduced amino acid sequence (522 amino acid residues) had 72·2% and 59·5% homology with rainbow trout and catfish P450arom respectively, and about 50% homology with mammalian and avian P450arom. Expression of this cDNA in COS-7 cells produced a protein that converted exogenous testosterone to estrogens. Northern blots using a tilapia P450arom cDNA fragment and Western blots using an antiserum against a tilapia P450arom polypeptide fragment revealed a single P450arom mRNA (2·6 kb) and a single protein (59 kDa) in tilapia ovarian tissue respectively. These analyses also revealed that the levels of both P450arom mRNA and protein were low in early vitellogenic follicles, increased in midvitellogenic follicles, and declined to non-detectable levels in post-vitellogenic follicles. Changes in the ability of follicles to convert exogenous testosterone to estrogens (aromatase activity) were similar to those of P450arom mRNA and protein. These observations indicated that the capacity of tilapia ovarian follicles to synthesize estradiol-17β is closely related to the contents of P450arom mRNA and protein within them.

Closely Related Genes Encode Developmental and Tissue Isoforms of Porcine Cytochrome P450 Aromatase

1997 ◽  
Vol 16 (6) ◽  
pp. 769-777 ◽  
Author(s):  
INHO CHOI ◽  
DERYL L. TROYER ◽  
DEAN L. CORNWELL ◽  
KATHY R. KIRBY-DOBBELS ◽  
WERNER R. COLLANTE ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
P450 Aromatase ◽  
Cytochrome P450 Aromatase
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