The use of multiple endpoints to assess cellular responses to environmental contaminants in the interstitial marine ciliate Euplotes crassus

2012 ◽  
Vol 114-115 ◽  
pp. 206-216 ◽  
Author(s):  
A. Gomiero ◽  
S. Sforzini ◽  
A. Dagnino ◽  
C. Nasci ◽  
A. Viarengo
Keyword(s):  
Environmental Contaminants ◽  
Cellular Responses ◽  
Marine Ciliate ◽  
Multiple Endpoints ◽  
Euplotes Crassus

scholarly journals Antimicrobial Activity of Euplotin C, the Sesquiterpene Taxonomic Marker from the Marine Ciliate Euplotes crassus

2004 ◽  
Vol 48 (10) ◽  
pp. 3828-3833 ◽  
Author(s):  
Dianella Savoia ◽  
Claudio Avanzini ◽  
Tiziano Allice ◽  
Emanuela Callone ◽  
Graziano Guella ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Leishmania Major ◽  
Lethal Dose ◽  
J774 Cell ◽  
Marine Ciliate ◽  
Euplotes Crassus ◽  
Taxonomic Marker ◽  
Solubility Enhancers ◽  
J774 Cell Line ◽  
Lethal Doses

ABSTRACT Strains of the marine ciliate protist Euplotes crassus produce exclusive terpenoids called euplotins that play an ecological role. Among these derivatives, euplotin C is the main of four secondary metabolites isolated from cultures of this protozoon and represents the sesquiterpene taxonomic marker from E. crassus. Because different terpenoid metabolites of plant origin showed a certain antimicrobial activity, we assessed the compound euplotin C, purified by high-pressure liquid chromatography and solubilized in two solubility enhancers, against the protozoa Leishmania major and Leishmani infantum, the fungus Candida albicans, and nine strains of gram-positive and gram-negative microorganisms. An activity of euplotin C against Leishmania promastigotes was demonstrated (50% lethal doses were 4.6 or 8.1 μg/ml depending on the agent used to solubilize the compound), while the effect was less evident on Candida and nearly absent on bacteria. A nonsignificant cytotoxicity (50% lethal dose, >200 μg/ml) against the J774 cell line was observed. A leishmanicidal activity was also shown by the living, euplotin-producing cells of E. crassus cultured together with promastigotes; this activity increased with time from 10 min to 6 h of incubation. This study provides an initial rationale for the evaluation of euplotin C and other similar natural products as alternative or possibly synergistic compounds for current antiprotozoon chemotherapeutics.

scholarly journals Anticancer Activity of Euplotin C, Isolated from the Marine Ciliate Euplotes crassus, Against Human Melanoma Cells

Marine Drugs ◽  
2018 ◽  
Vol 16 (5) ◽  
pp. 166 ◽  
Author(s):  
Sara Carpi ◽  
Beatrice Polini ◽  
Giulio Poli ◽  
Gabriela Alcantara Barata ◽  
Stefano Fogli ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Marine Ciliate ◽  
Euplotes Crassus ◽  
Human Melanoma Cells

Cellular responses to environmental contaminants in amoebic cells of the slime mould Dictyostelium discoideum

2006 ◽  
Vol 143 (2) ◽  
pp. 150-157 ◽  
Author(s):  
Francesco Dondero ◽  
Henrik Jonsson ◽  
Mauro Rebelo ◽  
Gabriella Pesce ◽  
Elena Berti ◽  
...  
Keyword(s):  
Dictyostelium Discoideum ◽  
Environmental Contaminants ◽  
Cellular Responses ◽  
Slime Mould
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