Abstract:
Introduction: Research has made progress in organ fabrication
using an extracellular matrix, cell sheets, or organoids. Human liver tissue has
been constructed using a 3-dimensional (3D) bioprinter and showed evidence that
an in vitro generated liver bud was reformed in a rodent liver
model. This study describes the stages of development of rat fetal organs and
liver structure and reviews recent progress in liver organoid transplantation.
Methods: The authors developed the procedures for creating
a transected plane for use in experimental microsurgery in rats. A liver lobe
was fixed vertically with gauze and it was ligated with 6-0 silk suture in the
cut line; the parenchyma was cut, and major vessels were ligated to create the
transected plane. The ligated tissue was carefully resected. Hemostasis was not
required and hepatic components remained on the transected plane. The plane was
covered by omentum. Results: Using this model, we transplanted
fetal liver or a 3D bioprinted liver organoid. This microsurgical method enabled
creation of an intact liver parenchyma plane. No bleeding was observed. The
transplanted liver components successfully engrafted on the liver.
Conclusion: This method may provide an essential
environment for growing liver using portal and arterial blood flow.
Maxillofacial bone regeneration with osteogenic matrix cell sheets: An experimental study in rats
